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  4. BODIPY-581/591 NHS ester

BODIPY-581/591 NHS ester is a bright, red fluorescent dye (excitation: 581 nm; emission: 591 nm). BODIPY-581/591 NHS ester shows unique hydrophobic properties for staining lipids, membranes, and other lipophilic compounds.

For research use only. We do not sell to patients.

BODIPY-581/591 NHS ester Chemical Structure

BODIPY-581/591 NHS ester Chemical Structure

CAS No. : 150152-70-8

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1 mg USD 110 In-stock
5 mg USD 280 In-stock
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Based on 1 publication(s) in Google Scholar

BODIPY-581/591 NHS ester Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE BODIPY-581/591 NHS ester

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BODIPY-581/591 NHS ester is a bright, red fluorescent dye (excitation: 581 nm; emission: 591 nm). BODIPY-581/591 NHS ester shows unique hydrophobic properties for staining lipids, membranes, and other lipophilic compounds[1].

Molecular Weight

489.28

Formula

C26H22BF2N3O4
CAS No.
Appearance

Solid

Color

Pale purple to purple

Emission (Em)

591
Excitation (Ex)

581
SMILES

O=C(ON(C1=O)C(CC1)=O)CCC2=[N-]([B+3]([F-])([N]34)[F-])C(C=C2)=CC3=CC=C4/C=C/C=C/C5=CC=CC=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (51.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0438 mL 10.2191 mL 20.4382 mL
5 mM 0.4088 mL 2.0438 mL 4.0876 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1.25 mg/mL (2.55 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.67%

COA (253 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0438 mL 10.2191 mL 20.4382 mL 51.0955 mL
5 mM 0.4088 mL 2.0438 mL 4.0876 mL 10.2191 mL
10 mM 0.2044 mL 1.0219 mL 2.0438 mL 5.1095 mL
15 mM 0.1363 mL 0.6813 mL 1.3625 mL 3.4064 mL
20 mM 0.1022 mL 0.5110 mL 1.0219 mL 2.5548 mL
25 mM 0.0818 mL 0.4088 mL 0.8175 mL 2.0438 mL
30 mM 0.0681 mL 0.3406 mL 0.6813 mL 1.7032 mL
40 mM 0.0511 mL 0.2555 mL 0.5110 mL 1.2774 mL
50 mM 0.0409 mL 0.2044 mL 0.4088 mL 1.0219 mL
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BODIPY-581/591 NHS ester Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY-581/591 NHS ester150152-70-8Fluorescent Dyeexcitation: 581 nmemission: 591 nmhydrophobic propertystaining lipidsInhibitorinhibitorinhibit

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