Phenosafranine

Cat. No.: HY-W040198 Purity: 80.12%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Phenosafranine is a phenazine dye. Phenosafranine has high binding affinity to triplex RNA compared to the parent duplex form, binds through intercalation to both forms of RNA. Phenosafranine can be used for staining plant cells, determination of hemoglobin, dopamine, serotonin and so on.

For research use only. We do not sell to patients.

Phenosafranine Chemical Structure

CAS No. : 81-93-6

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Description

Molecular Weight

322.79

Formula

C₁₈H₁₅ClN₄

CAS No.

81-93-6

Appearance

Solid

Color

Green to dark green

SMILES

NC1=CC2=[N+](C3=C(N=C2C=C1)C=CC(N)=C3)C4=CC=CC=C4.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (387.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0980 mL	15.4899 mL	30.9799 mL
5 mM	0.6196 mL	3.0980 mL	6.1960 mL
10 mM	0.3098 mL	1.5490 mL	3.0980 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 80.12%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (249 KB) RP-HPLC (249 KB) LCMS (103 KB)

Handling Instructions (2659 KB)

References

[1]. Widholm JM. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 1972 Jul;47(4):189-94. [Content Brief]

[2]. Liu, W., et al. Characterization of Phenosafranine–Hemoglobin Interactions in Aqueous Solution. J Solution Chem 40, 231–246 (2011).

[3]. Pradhan AB, et al. An overview on the interaction of phenazinium dye phenosafranine to RNA triple and double helices. Int J Biol Macromol. 2016 May;86:345-51. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0980 mL	15.4899 mL	30.9799 mL	77.4497 mL
	5 mM	0.6196 mL	3.0980 mL	6.1960 mL	15.4899 mL
	10 mM	0.3098 mL	1.5490 mL	3.0980 mL	7.7450 mL
	15 mM	0.2065 mL	1.0327 mL	2.0653 mL	5.1633 mL
	20 mM	0.1549 mL	0.7745 mL	1.5490 mL	3.8725 mL
	25 mM	0.1239 mL	0.6196 mL	1.2392 mL	3.0980 mL
	30 mM	0.1033 mL	0.5163 mL	1.0327 mL	2.5817 mL
	40 mM	0.0774 mL	0.3872 mL	0.7745 mL	1.9362 mL
	50 mM	0.0620 mL	0.3098 mL	0.6196 mL	1.5490 mL
	60 mM	0.0516 mL	0.2582 mL	0.5163 mL	1.2908 mL
	80 mM	0.0387 mL	0.1936 mL	0.3872 mL	0.9681 mL
	100 mM	0.0310 mL	0.1549 mL	0.3098 mL	0.7745 mL

Phenosafranine Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenosafranine81-93-6Fluorescent DyephenazineRNA bindingstainingInhibitorinhibitorinhibit

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