PHPS1

Name: PHPS1
Brand: MedChemExpress
SKU: HY-112368
Rating: 5.0 (2 reviews)

Cat. No.: HY-112368 Purity: 99.77%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

PHPS1 is a potent and selective Shp2 inhibitor with K_is of 0.73, 5.8, 10.7, 5.8, and 0.47 μM for Shp2, Shp2-R362K, Shp1, PTP1B, and PTP1B-Q, respectively.

For research use only. We do not sell to patients.

PHPS1 Chemical Structure

CAS No. : 314291-83-3

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 570	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 860	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of PHPS1:

PHPS1 sodium Get quote

2 Publications Citing Use of MCE PHPS1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

PHPS1 is a potent and selective Shp2 inhibitor with K_is of 0.73, 5.8, 10.7, 5.8, and 0.47 μM for Shp2, Shp2-R362K, Shp1, PTP1B, and PTP1B-Q, respectively^[1].

IC₅₀ & Target

Ki: 0.73 μM (Shp2), 5.8 μM (Shp2-R362K), 10.7 μM (Shp1), 5.8 μM (PTP1B), 0.47 μM (PTP1B-Q)^[1]

In Vitro

PHPS1 (30 μM; 6 days) inhibits proliferation of human tumor cells^[1].
PHPS1 (5-20 μM; 5-360 minutes) inhibits Erk1/2 but not Akt and Stat3 phosphorylation in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Human cancer cell lines MDA-MB-435, HCT-116 (colon carcinoma), HCT-15 (colon carcinoma), PC-3 (prostate carcinoma) HT-29 (colon carcinoma), NCI-H661 (lung carcinoma), and Caki-1 (kidney carcinoma)
Concentration:	30 μM
Incubation Time:	6 days
Result:	Resulted in a reduction in cell number of between 0% (Caki-1) to 74% (HT-29).

Western Blot Analysis^[1]

Cell Line:	Madin-Darby canine kidney (MDCK) cells
Concentration:	5, 10, 20 μM
Incubation Time:	5, 15, 60, 120, 360 minutes
Result:	Inhibited HGF/SF (1 unit/mL)-induced phosphorylation and thus activation of Erk1/2 over a time period of 15 min to 6 h. In contrast, transient phosphorylation of Erk1/2 after 5 min was not affected. Exhibited no effect on HGF/SF-induced activation of PI3K/Akt or Stat3.

In Vivo

PHPS1 (3 mg/kg; i.p. injection; every day during the last week on the high-fat diet) renders Ldlr^-/- mice less susceptible to atherosclerosis development^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Ldlr^-/- (005061) mice^[2]
Dosage:	3 mg/kg
Administration:	Intraperitoneal (i.p.) injection; every day during the last week on the high-fat diet.
Result:	Revealed a significant decrease in atherosclerotic plaque size in the aorta compared with the other two groups.

Molecular Weight

465.44

Formula

C₂₁H₁₅N₅O₆S

CAS No.

314291-83-3

Appearance

Solid

Color

Red to reddish brown

SMILES

O=S(C1=CC=C(N/N=C2C(C3=CC=C([N+]([O-])=O)C=C3)=NN(C4=CC=CC=C4)C\2=O)C=C1)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (53.71 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1485 mL	10.7425 mL	21.4850 mL
5 mM	0.4297 mL	2.1485 mL	4.2970 mL
10 mM	0.2149 mL	1.0743 mL	2.1485 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (257 KB) RP-HPLC (260 KB)

Handling Instructions (2659 KB)

References

[1]. Klaus Hellmuth, et al. Specific Inhibitors of the Protein Tyrosine Phosphatase Shp2 Identified by High-Throughput Docking. Proc Natl Acad Sci U S A. 2008 May 20;105(20):7275-80. [Content Brief]

[2]. Jia Chen, et al. SHP2 Inhibitor PHPS1 Protects Against Atherosclerosis by Inhibiting Smooth Muscle Cell Proliferation. BMC Cardiovasc Disord. 2018 Apr 27;18(1):72. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1485 mL	10.7425 mL	21.4850 mL	53.7126 mL
	5 mM	0.4297 mL	2.1485 mL	4.2970 mL	10.7425 mL
	10 mM	0.2149 mL	1.0743 mL	2.1485 mL	5.3713 mL
	15 mM	0.1432 mL	0.7162 mL	1.4323 mL	3.5808 mL
	20 mM	0.1074 mL	0.5371 mL	1.0743 mL	2.6856 mL
	25 mM	0.0859 mL	0.4297 mL	0.8594 mL	2.1485 mL
	30 mM	0.0716 mL	0.3581 mL	0.7162 mL	1.7904 mL
	40 mM	0.0537 mL	0.2686 mL	0.5371 mL	1.3428 mL
	50 mM	0.0430 mL	0.2149 mL	0.4297 mL	1.0743 mL

PHPS1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

PHPS1

Customer Review

Other Forms of PHPS1:

PHPS1 Related Antibodies

2 Publications Citing Use of MCE PHPS1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

PHPS1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

PHPS1 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report