Propranolol

Name: Propranolol
Brand: MedChemExpress
SKU: HY-B0573B
Rating: 5.0 (23 reviews)

Cat. No.: HY-B0573B Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

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Propranolol is a nonselective β-adrenergic receptor (βAR) antagonist, has high affinity for the β1AR and β2AR with K_i values of 1.8 nM and 0.8 nM, respectively. Propranolol inhibits [³H]-DHA binding to rat brain membrane preparation with an IC₅₀ of 12 nM. Propranolol is used for the study of hypertension, pheochromocytoma, myocardial infarction, cardiac arrhythmias, angina pectoris, and hypertrophic cardiomyopathy.

For research use only. We do not sell to patients.

Propranolol Chemical Structure

CAS No. : 525-66-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 165	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 165	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
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Based on 23 publication(s) in Google Scholar

Other Forms of Propranolol:

Propranolol hydrochloride In-stock
Propranolol-d₇ Get quote

23 Publications Citing Use of MCE Propranolol

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α adrenergic receptor β adrenergic receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Propranolol is a nonselective β-adrenergic receptor (βAR) antagonist, has high affinity for the β1AR and β2AR with K_i values of 1.8 nM and 0.8 nM, respectively^[1]. Propranolol inhibits [³H]-DHA binding to rat brain membrane preparation with an IC₅₀ of 12 nM^[2]. Propranolol is used for the study of hypertension, pheochromocytoma, myocardial infarction, cardiac arrhythmias, angina pectoris, and hypertrophic cardiomyopathy^[3].

IC₅₀ & Target

β adrenergic receptor

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	59.5 μM Compound: Propranolol	Antiproliferative activity against human A375 cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay Antiproliferative activity against human A375 cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay	[PMID: 32216987]
CHO	IC₅₀	> 300 μM Compound: 14d	Cytotoxicity against CHO cells after 48 hrs by MTT assay Cytotoxicity against CHO cells after 48 hrs by MTT assay	[PMID: 22196513]
HEK293	IC₅₀	0.48 nM Compound: Prop	Antagonist activity at ADRB2 endogenously expressed in HEK293 cells transfected with cAMP FRET biosensor assessed as inhibition of cimaterol-induced response pre-incubated for 45 mins under constant violet light conditions by FRET assay Antagonist activity at ADRB2 endogenously expressed in HEK293 cells transfected with cAMP FRET biosensor assessed as inhibition of cimaterol-induced response pre-incubated for 45 mins under constant violet light conditions by FRET assay	[PMID: 32686936]
HEK293	IC₅₀	0.55 nM Compound: Prop	Antagonist activity at ADRB2 endogenously expressed in HEK293 cells transfected with cAMP FRET biosensor assessed as inhibition of cimaterol-induced response pre-incubated for 45 mins under dark conditions by FRET assay Antagonist activity at ADRB2 endogenously expressed in HEK293 cells transfected with cAMP FRET biosensor assessed as inhibition of cimaterol-induced response pre-incubated for 45 mins under dark conditions by FRET assay	[PMID: 32686936]
HEK293	IC₅₀	21 μM Compound: Propranolol	Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit	[PMID: 22761000]
HEK293	IC₅₀	2100 nM Compound: Propranolol	Inhibition of sodium current measured using whole-cell patch clamp experiments in HEK-293 cells stably transfected with hNaV1.5 cDNA Inhibition of sodium current measured using whole-cell patch clamp experiments in HEK-293 cells stably transfected with hNaV1.5 cDNA	[PMID: 21300721]
HEK293	IC₅₀	63.2 μM Compound: propranolol	Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy	[PMID: 18788725]
HMEC-1	GI₅₀	149 μM Compound: Propranolol	Antiproliferative activity against human HMEC1 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human HMEC1 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
MDA-MB-231	GI₅₀	205 μM Compound: Propranolol	Antiproliferative activity against human MDA-MB-231 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
MRC5	GI₅₀	150 μM Compound: Propranolol	Antiproliferative activity against human MRC5 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human MRC5 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
SK-MEL-28	IC₅₀	75.82 μM Compound: Propranolol	Antiproliferative activity against human SK-MEL-28 cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay Antiproliferative activity against human SK-MEL-28 cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay	[PMID: 32216987]
SK-MEL-5	IC₅₀	62.21 μM Compound: Propranolol	Antiproliferative activity against human SK-MEL-5 cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay Antiproliferative activity against human SK-MEL-5 cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay	[PMID: 32216987]
T98G	IC₅₀	75 μM Compound: Rac-22	Antiproliferation activity against human T98G cells measured after 72 hrs by crystal violet staining based assay Antiproliferation activity against human T98G cells measured after 72 hrs by crystal violet staining based assay	[PMID: 34115934]
Ventricular myocyte	IC₅₀	18 μM Compound: Propranolol	Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes	[PMID: 22761000]
Ventricular myocyte	IC₅₀	18000 nM Compound: Propranolol	Inhibition of calcium current (ICaL) measured using whole-cell patch clamp experiments in isolated guinea pig ventricular myocytes Inhibition of calcium current (ICaL) measured using whole-cell patch clamp experiments in isolated guinea pig ventricular myocytes	[PMID: 21300721]

In Vitro

Propranolol (10^-7 M-10^-3 M; 24 and 48 hours) increases the total ERK1/2 levels in a dose-dependent manner, and ERK1/2 activation is observed specifically at 10^-5 M in HemSCs^[4].
? Propranolol (10^-9 M-10^-3 M; 24 and 48 hours) significant decreases cell proliferation at 10^-4 M propranolol after 24 hours and 10^-9 M propranolol after 48 hours in HemSCs^[4].
? Propranolol (50 μM-200 μM；24 hours) increases Annexin V positivity and caspase-3 activation, rapidly induces HemSC apoptosis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:	HemSC cells
Concentration:	10^-7 M-10^-3 M
Incubation Time:	24 and 48 hours
Result:	Increased the total ERK1/2 levels in a dose-dependent manner.

Cell Proliferation Assay^[4]

Cell Line:	HemSC cells
Concentration:	10^-9 M-10^-3 M
Incubation Time:	24 and 48 hours
Result:	Suppressed HemSC proliferation.

Apoptosis Analysis^[4]

Cell Line:	HemSC cells
Concentration:	50 μM, 100 μM, or 200 μM
Incubation Time:	24 hours
Result:	Induced HemSC cell death occurred via an apoptotic pathway.

In Vivo

Propranolol (orally administration; 40 mg/kg; daily) significantly reduces the vessel diameter relative to the vehicle-treated implants, and increases the number of cells that expressed phosphorylated ERK1/2 within the IH Matrigel implant^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	A xenograft mouse model of IH (infantile hemangiomas ) with HemSC cells^[4]
Dosage:	40 mg/kg
Administration:	Orally administration; 40 mg/kg; daily
Result:	Improved vessel development in the IH mouse model that correlated with MAPK pathway activation.

Clinical Trial

Molecular Weight

259.34

Formula

C₁₆H₂₁NO₂

CAS No.

525-66-6

Appearance

Solid

Color

White to off-white

SMILES

OC(COC1=C2C=CC=CC2=CC=C1)CNC(C)C

Structure Classification

Alkaloids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (385.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8559 mL	19.2797 mL	38.5594 mL
5 mM	0.7712 mL	3.8559 mL	7.7119 mL
10 mM	0.3856 mL	1.9280 mL	3.8559 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (245 KB) HNMR (247 KB) LCMS (251 KB)

Handling Instructions (2659 KB)

References

[1]. Galandrin S, et al. Distinct signaling profiles of beta1 and beta2 adrenergic receptor ligands toward adenylyl cyclase and mitogen-activated protein kinase reveals the pluridimensionality of efficacy. Mol Pharmacol. 2006 Nov;70(5):1575-84. Epub 2006 Aug 1 [Content Brief]

[2]. Briley M, et al. Evidence against beta-adrenoceptor blocking activity of diltiazem, a drug with calcium antagonist properties. Br J Pharmacol. 1980 Aug;69(4):669-73. [Content Brief]

[3]. Al-Majed AA, et al. Propranolol. Profiles Drug Subst Excip Relat Methodol. 2017;42:287-338. [Content Brief]

[4]. Munabi NC, et al. Propranolol Targets Hemangioma Stem Cells via cAMP and Mitogen-Activated Protein Kinase Regulation. Stem Cells Transl Med. 2016 Jan;5(1):45-55. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8559 mL	19.2797 mL	38.5594 mL	96.3986 mL
	5 mM	0.7712 mL	3.8559 mL	7.7119 mL	19.2797 mL
	10 mM	0.3856 mL	1.9280 mL	3.8559 mL	9.6399 mL
	15 mM	0.2571 mL	1.2853 mL	2.5706 mL	6.4266 mL
	20 mM	0.1928 mL	0.9640 mL	1.9280 mL	4.8199 mL
	25 mM	0.1542 mL	0.7712 mL	1.5424 mL	3.8559 mL
	30 mM	0.1285 mL	0.6427 mL	1.2853 mL	3.2133 mL
	40 mM	0.0964 mL	0.4820 mL	0.9640 mL	2.4100 mL
	50 mM	0.0771 mL	0.3856 mL	0.7712 mL	1.9280 mL
	60 mM	0.0643 mL	0.3213 mL	0.6427 mL	1.6066 mL
	80 mM	0.0482 mL	0.2410 mL	0.4820 mL	1.2050 mL
	100 mM	0.0386 mL	0.1928 mL	0.3856 mL	0.9640 mL

Propranolol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Propranolol525-66-6Adrenergic ReceptorBacterialBeta ReceptorInhibitorinhibitorinhibit

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Propranolol

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23 Publications Citing Use of MCE Propranolol

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References

Customer Review

Propranolol Related Antibodies

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Complete Stock Solution Preparation Table

Propranolol Related Classifications

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