Rifampicin (Synonyms: Rifampin; Rifamycin AMP)

Name: Rifampicin
Brand: MedChemExpress
SKU: HY-B0272
Rating: 5.0 (25 reviews)

Cat. No.: HY-B0272 Purity: 98.15%: FAQs
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Rifampicin is a potent and broad spectrum antibiotic against bacterial pathogens. Rifampicin has anti-influenza virus activities. Rifampicin shows anti-orthopoxvirus activity.

For research use only. We do not sell to patients.

Rifampicin Chemical Structure

CAS No. : 13292-46-1

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 25 publication(s) in Google Scholar

Other Forms of Rifampicin:

Rifampicin-d₃ Get quote
Rifampicin-d₈ Get quote
Rifampicin-d₄ Get quote
Rifampicin-d₁₁ Get quote
Rifampicin (Standard) Get quote

23 Publications Citing Use of MCE Rifampicin

: Rifampicin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2019 Mar 15;56:175-182. [Abstract]; Representative Western blots for P-gp、BCRP、MRP2 in LS-180 treated with six active compounds in liquorice. C: control, P-1: Rifampicin, P-2: Bosentan, S-1: Liquiritin, S-2: Liquiritigenin, S-3: Isoliquiritin, S-4: Isoliquiritigenin, S-5: Glycyrrhetinic acid, S-6: Licochalcone A.

: Rifampicin purchased from MedChemExpress. Usage Cited in: Onco Targets Ther. 2018 Sep 17;11:5885-5894. [Abstract]; MHCC97-H cells are treated with the indicated concentrations of Rifampicin for 48 hours. Then, cells are harvested for Western blot analysis.

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Description

Rifampicin is a potent and broad spectrum antibiotic against bacterial pathogens. Rifampicin has anti-influenza virus activities. Rifampicin shows anti-orthopoxvirus activity.

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against human A549 cells incubated for 1 to 11 days by MTT assay Cytotoxicity against human A549 cells incubated for 1 to 11 days by MTT assay	10.1039/C5MD00404G
A549	GI₅₀	> 100 μg/mL Compound: Rifampicin	Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 28110868]
A549	GI₅₀	> 100 μg/mL Compound: RP	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 27013391]
A549	GI₅₀	> 100 μg/mL Compound: RP	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 30910461]
A549	GI₅₀	> 100 μg/mL Compound: Rifampicin	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 28411559]
A549	GI₅₀	> 121.51 mM Compound: RP	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	10.1039/C6MD00278A
B-cell	IC₅₀	96 μM Compound: Rifampin	Antiproliferative activity against Theileria parva-induced proliferation of bovine B lymphocyte assessed as inhibition of [3H]thymidine uptake after 48 hrs Antiproliferative activity against Theileria parva-induced proliferation of bovine B lymphocyte assessed as inhibition of [3H]thymidine uptake after 48 hrs	[PMID: 19075064]
CHO	CC₅₀	512 μM Compound: RIF	Cytotoxicity against CHO cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against CHO cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31525660]
HCT-116	GI₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against human HCT116 cells incubated for 1 to 11 days by MTT assay Cytotoxicity against human HCT116 cells incubated for 1 to 11 days by MTT assay	10.1039/C5MD00404G
HCT-116	GI₅₀	> 100 μg/mL Compound: Rifampicin	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 28110868]
HCT-116	GI₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	[PMID: 27914801]
HCT-116	CC₅₀	> 100 μg/mL Compound: Rifampicin	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	[PMID: 27301367]
HCT-116	GI₅₀	> 121.51 mM Compound: RP	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	10.1039/C6MD00278A
HEK293	CC₅₀	> 100 μM Compound: Rifampicin	Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue reporter assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue reporter assay	[PMID: 30392371]
HEK293	IC₅₀	0.3 μM Compound: Rifampicin	Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells using estradiol-17beta-glucuronide substrate Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells using estradiol-17beta-glucuronide substrate	[PMID: 22587986]
HEK293	EC₅₀	1.1 μM Compound: Rifampicin	Agonist activity at human PXR transfected in HEK293 cells after 24 hrs by luciferase reporter gene assay Agonist activity at human PXR transfected in HEK293 cells after 24 hrs by luciferase reporter gene assay	[PMID: 26905831]
HEK293	IC₅₀	1.2 μM Compound: Rifampicin	Inhibition of human liver OATP1B1 expressed in HEK293 Flp-In cells assessed as reduction in E17-betaG uptake by scintillation counting Inhibition of human liver OATP1B1 expressed in HEK293 Flp-In cells assessed as reduction in E17-betaG uptake by scintillation counting	[PMID: 22541068]
HEK293	IC₅₀	1.5 μM Compound: Rifampicin	Inhibition of human liver OATP1B3 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E17-betaG uptake incubated for 5 mins by scintillation counting Inhibition of human liver OATP1B3 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E17-betaG uptake incubated for 5 mins by scintillation counting	[PMID: 22541068]
HEK293	IC₅₀	2.2 μM Compound: Rifampicin	Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells using pitavastatin substrate Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells using pitavastatin substrate	[PMID: 22587986]
HEK293	IC₅₀	2.3 μM Compound: Rifampicin	Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells using estrone-3-sulfate substrate Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells using estrone-3-sulfate substrate	[PMID: 22587986]
HEK293	IC₅₀	65 μM Compound: Rifampicin	Inhibition of human liver OATP2B1 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E3S uptake incubated for 5 mins by scintillation counting Inhibition of human liver OATP2B1 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E3S uptake incubated for 5 mins by scintillation counting	[PMID: 22541068]
HeLa	GI₅₀	> 100 μg/mL Compound: RP	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 27013391]
HeLa	GI₅₀	> 100 μg/mL Compound: RP	Growth inhibition of human HeLa cells by MTT assay Growth inhibition of human HeLa cells by MTT assay	[PMID: 30910461]
HeLa	GI₅₀	> 100 μg/mL Compound: Rifampicin	Growth inhibition of human HeLa cells by MTT assay Growth inhibition of human HeLa cells by MTT assay	[PMID: 28411559]
HeLa	CC₅₀	> 100 μg/mL Compound: Rifampicin	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 27301367]
HeLa	IC₅₀	> 20 μM Compound: RIF	Cytotoxicity against human HeLa cells assessed as cell viability after 24 hrs by MTS assay Cytotoxicity against human HeLa cells assessed as cell viability after 24 hrs by MTS assay	[PMID: 26948407]
HeLa	IC₅₀	> 20 μM Compound: RIF	Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTS assay Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTS assay	[PMID: 26948407]
HeLa	IC₅₀	270 μg/mL Compound: rifampin	Antiproliferative effect against HeLa cells after 48 hrs Antiproliferative effect against HeLa cells after 48 hrs	[PMID: 17088489]
Hepatocyte	IC₅₀	> 20 μM Compound: RIF	Cytotoxicity against primary human hepatocytes assessed as cell viability after 48 hrs by MTS assay Cytotoxicity against primary human hepatocytes assessed as cell viability after 48 hrs by MTS assay	[PMID: 26948407]
Hepatocyte	EC₅₀	0.98 μM Compound: Rifampicin	Induction of CYP2B6 mRNA expression in human donor hepatocytes after 48 hrs by real-time PCR method Induction of CYP2B6 mRNA expression in human donor hepatocytes after 48 hrs by real-time PCR method	[PMID: 22815312]
Hepatocyte	EC₅₀	1.3 μM Compound: Rifampicin	Induction of CYP2B6 activity in human donor hepatocytes assessed as hydroxybupropion formation after 48 hrs by LC/MS method Induction of CYP2B6 activity in human donor hepatocytes assessed as hydroxybupropion formation after 48 hrs by LC/MS method	[PMID: 22815312]
HepG2	EC₅₀	0.19 μM Compound: Rifampicin	Agonist activity at PXR expressed in human HepG2 cells after 24 hrs by p3A4-luciferase reporter gene assay Agonist activity at PXR expressed in human HepG2 cells after 24 hrs by p3A4-luciferase reporter gene assay	[PMID: 27145071]
HepG2	IC₅₀	145.33 μM Compound: Rifampicin	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay	[PMID: 32249118]
HepG2	CC₅₀	175.9 μg/mL Compound: RIF	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33940463]
HepG2	IC₅₀	22.2 μM Compound: 75	Antimalarial activity against liver stages of Plasmodium yoelii 17XNL expressing GFP infected in human HepG2 cells transfected with CD81 assessed as growth inhibition of hepatic parasite after 40 hrs by flow cytometry Antimalarial activity against liver stages of Plasmodium yoelii 17XNL expressing GFP infected in human HepG2 cells transfected with CD81 assessed as growth inhibition of hepatic parasite after 40 hrs by flow cytometry	[PMID: 22122518]
HepG2	EC₅₀	3.2 μM Compound: Rifampicin	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	3.5 μM Compound: Rifampicin	Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
Huh-7	IC₅₀	> 20 μM Compound: RIF	Cytotoxicity against human HuH7 cells assessed as cell viability after 24 hrs by MTS assay Cytotoxicity against human HuH7 cells assessed as cell viability after 24 hrs by MTS assay	[PMID: 26948407]
Huh-7	IC₅₀	> 20 μM Compound: RIF	Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by MTS assay Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by MTS assay	[PMID: 26948407]
J774.A1	IC₅₀	> 100 μM Compound: RIF	Cytotoxicity against mouse J774A1 cells assessed as cell viability after 24 hrs by Trypan blue exclusion assay Cytotoxicity against mouse J774A1 cells assessed as cell viability after 24 hrs by Trypan blue exclusion assay	[PMID: 24611928]
J774.A1	IC₅₀	> 64 μg/mL Compound: RFP	Cytotoxicity against mouse J774.A1 cells after 48 hrs by MTT assay Cytotoxicity against mouse J774.A1 cells after 48 hrs by MTT assay	[PMID: 36150342]
J774.A1	CC₅₀	207.03 μg/mL Compound: rifampin	Cytotoxicity against mouse J774A1 cells after 24 to 72 hrs by MTT assay Cytotoxicity against mouse J774A1 cells after 24 to 72 hrs by MTT assay	[PMID: 20934344]
Macrophage	IC₅₀	> 100 μM Compound: Rifampicin	Cytotoxicity against mouse bone marrow derived macrophage assessed as growth inhibition after 48 hrs by MTS assay Cytotoxicity against mouse bone marrow derived macrophage assessed as growth inhibition after 48 hrs by MTS assay	[PMID: 23454512]
MCF7	GI₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against human MCF7 cells incubated for 1 to 11 days by MTT assay Cytotoxicity against human MCF7 cells incubated for 1 to 11 days by MTT assay	10.1039/C5MD00404G
MCF7	GI₅₀	> 100 μg/mL Compound: Rifampicin	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 28110868]
MCF7	GI₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 27914801]
MCF7	GI₅₀	> 121.51 mM Compound: RP	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	10.1039/C6MD00278A
MDCK-II	IC₅₀	> 20 μM Compound: RIF	Cytotoxicity against MDCK2-MDR1 cells assessed as cell viability after 24 hrs by MTS assay Cytotoxicity against MDCK2-MDR1 cells assessed as cell viability after 24 hrs by MTS assay	[PMID: 26948407]
MDCK-II	IC₅₀	> 20 μM Compound: RIF	Cytotoxicity against MDCK2-MDR1 cells assessed as cell viability after 48 hrs by MTS assay Cytotoxicity against MDCK2-MDR1 cells assessed as cell viability after 48 hrs by MTS assay	[PMID: 26948407]
MG-63	IC₅₀	240 μg/mL Compound: rifampin	Antiproliferative effect against MG63 cells assessed as BrdU incorporation into DNA after 48 hrs after 48 hrs Antiproliferative effect against MG63 cells assessed as BrdU incorporation into DNA after 48 hrs after 48 hrs	[PMID: 17088489]
MRC5	IC₅₀	> 100 μM Compound: Rifampicin	Cytotoxicity against human MRC5 cells assessed as cell growth inhibition after 48 hrs by resazurin dye reduction method Cytotoxicity against human MRC5 cells assessed as cell growth inhibition after 48 hrs by resazurin dye reduction method	[PMID: 26213786]
Osteoblast	IC₅₀	130 μg/mL Compound: rifampin	Antiproliferative effect against primary human osteoblasts assessed as BrdU incorporation into DNA after 48 hrs Antiproliferative effect against primary human osteoblasts assessed as BrdU incorporation into DNA after 48 hrs	[PMID: 17088489]
PANC-1	GI₅₀	> 100 μg/mL Compound: RP	Cytotoxicity against human PANC1 cells after 48 hrs by MTT assay Cytotoxicity against human PANC1 cells after 48 hrs by MTT assay	[PMID: 27013391]
PANC-1	GI₅₀	> 100 μg/mL Compound: RP	Growth inhibition of human PANC1 cells by MTT assay Growth inhibition of human PANC1 cells by MTT assay	[PMID: 30910461]
PANC-1	GI₅₀	> 100 μg/mL Compound: Rifampicin	Growth inhibition of human PANC1 cells by MTT assay Growth inhibition of human PANC1 cells by MTT assay	[PMID: 28411559]
PANC-1	CC₅₀	> 100 μg/mL Compound: Rifampicin	Cytotoxicity against human PANC1 cells by MTT assay Cytotoxicity against human PANC1 cells by MTT assay	[PMID: 27301367]
PBMC	IC₅₀	62.92 μg/mL Compound: RIF	Cytotoxicity against human PBMC assessed as reduction in cell growth after 72 hrs by MTT assay Cytotoxicity against human PBMC assessed as reduction in cell growth after 72 hrs by MTT assay	[PMID: 32977201]
RAW264.7	CC₅₀	80.39 μg/mL Compound: RIF	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33940463]
RAW264.7	GI₅₀	850 μM Compound: RIF	Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay	10.1039/C3MD00251A
THP-1	GI₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against human THP1 cells incubated for 1 to 11 days by MTT assay Cytotoxicity against human THP1 cells incubated for 1 to 11 days by MTT assay	10.1039/C5MD00404G
THP-1	GI₅₀	> 100 μg/mL Compound: RP	Cytotoxicity against human THP1 cells after 48 hrs by MTT assay Cytotoxicity against human THP1 cells after 48 hrs by MTT assay	[PMID: 27013391]
THP-1	GI₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against human THP1 cells by MTT assay Cytotoxicity against human THP1 cells by MTT assay	[PMID: 27914801]
THP-1	CC₅₀	> 100 μg/mL Compound: Rifampicin	Cytotoxicity against human THP1 cells by MTT assay Cytotoxicity against human THP1 cells by MTT assay	[PMID: 27301367]
THP-1	IC₅₀	> 100 μM Compound: Rifampicin	Cytotoxicity against human THP1 cells assessed as cell growth inhibition after 48 hrs by resazurin dye reduction method Cytotoxicity against human THP1 cells assessed as cell growth inhibition after 48 hrs by resazurin dye reduction method	[PMID: 26213786]
THP-1	IC₅₀	0.0018 μg/mL Compound: Rifampicin	Anti-tubercular activity against rifampicin, isoniazid, ethambutol and pyrazinamide-sensitive dormant phage of Mycobacterium tuberculosis H37Ra ATCC 25177 infected in human THP1 cells by neutral red assay Anti-tubercular activity against rifampicin, isoniazid, ethambutol and pyrazinamide-sensitive dormant phage of Mycobacterium tuberculosis H37Ra ATCC 25177 infected in human THP1 cells by neutral red assay	[PMID: 26299346]
THP-1	IC₅₀	0.0021 μg/mL Compound: Rifampicin	Anti-tubercular activity against rifampicin, isoniazid, ethambutol and pyrazinamide-sensitive active phage of Mycobacterium tuberculosis H37Ra ATCC 25177 infected in human THP1 cells by neutral red assay Anti-tubercular activity against rifampicin, isoniazid, ethambutol and pyrazinamide-sensitive active phage of Mycobacterium tuberculosis H37Ra ATCC 25177 infected in human THP1 cells by neutral red assay	[PMID: 26299346]
THP-1	GI₅₀	0.14 μg/mL Compound: Rifampicin	Cytotoxicity against human THP1 cells assessed as growth inhibition by MTT assay Cytotoxicity against human THP1 cells assessed as growth inhibition by MTT assay	[PMID: 28103537]
Vero	IC₅₀	> 10 μg/mL Compound: RIP	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as inhibition of cell viability after 96 hr by MTT assay Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as inhibition of cell viability after 96 hr by MTT assay	10.1007/s00044-012-0074-2
Vero	IC₅₀	> 100 μg/mL Compound: RMP	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS-PMS assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS-PMS assay	[PMID: 24087924]
Vero	IC₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 20233660]
Vero	IC₅₀	> 100 μg/mL Compound: RIF	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19058970]
Vero	IC₅₀	> 100 μg/mL Compound: RMP	Cytotoxicity against African green monkey Vero cells measured after 72 hrs by CellTiter 96 aqueous non-radioactive cell proliferation assay Cytotoxicity against African green monkey Vero cells measured after 72 hrs by CellTiter 96 aqueous non-radioactive cell proliferation assay	[PMID: 30891129]
Vero	IC₅₀	> 100 μM Compound: Rifampicin	Cytotoxicity against african green monkey Vero cells assessed as growth inhibition after 24 hrs by MTS assay Cytotoxicity against african green monkey Vero cells assessed as growth inhibition after 24 hrs by MTS assay	[PMID: 23454512]
Vero	IC₅₀	> 100 μM Compound: Rifampicin	Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTS assay Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTS assay	[PMID: 27426865]
Vero	IC₅₀	> 100 μM Compound: RMP	Cytotoxicity against African green monkey Vero cells by MTS assay Cytotoxicity against African green monkey Vero cells by MTS assay	[PMID: 26748697]
Vero	IC₅₀	> 100 μM Compound: RMP	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTS/PMS assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTS/PMS assay	[PMID: 29259747]
Vero	IC₅₀	> 100 μM Compound: Rifampin	Cytotoxicity against african green monkey Vero cells at < 10 uM after 3 days Cytotoxicity against african green monkey Vero cells at < 10 uM after 3 days	[PMID: 22850215]
Vero	CC₅₀	> 128 μg/mL Compound: Rifampicin	Cytotoxicity against African green monkey Vero cells incubated for 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells incubated for 72 hrs by MTT assay	[PMID: 31352245]
Vero	CC₅₀	> 150 μg/mL Compound: Rifampicin	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 26498570]
Vero	IC₅₀	> 200 μg/mL Compound: RIF	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30145051]
Vero	CC₅₀	> 32 μg/mL Compound: Rifampicin	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31353295]
Vero	CC₅₀	> 50 μg/mL Compound: RIF	Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay	[PMID: 22691154]
Vero	IC₅₀	> 50 μM Compound: 52	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by tetrazolium dye assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by tetrazolium dye assay	[PMID: 28094223]
Vero	CC₅₀	> 500 μg/mL Compound: Rifampicin	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 23062712]
Vero	IC₅₀	> 62.5 μg/mL Compound: Rifampicin	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24721315]
Vero	IC₅₀	> 62.5 μg/mL Compound: Rifampin	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 23352268]
Vero	IC₅₀	> 62.5 μg/mL Compound: RIF	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 20056418]
Vero	IC₅₀	> 64 μg/mL Compound: RFP	Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay	[PMID: 36150342]
Vero	IC₅₀	> 64 μg/mL Compound: Rfp	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 34126456]
Vero	IC₅₀	> 74.5 μM Compound: Rifampicin	Cytotoxicity against african green monkey Vero after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero after 72 hrs by MTT assay	[PMID: 20576327]
Vero	CC₅₀	> 75.9 μM Compound: rifampicin	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as cell viability after 72 hr by MTT assay Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as cell viability after 72 hr by MTT assay	10.1007/s00044-011-9638-9
Vero	IC₅₀	> 75.9 μM Compound: RIF	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19131245]
Vero	CC₅₀	> 75.94 μM Compound: Rifampicin	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 20615698]
Vero	IC₅₀	> 75.94 μM Compound: Rifampicin	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19942326]
Vero	IC₅₀	> 75.94 μM Compound: rifampicin	Cytotoxicity against Vero cells after 72 hrs by MTT assay Cytotoxicity against Vero cells after 72 hrs by MTT assay	[PMID: 17276683]
Vero	IC₅₀	> 77.4 μM Compound: Rifampicin	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells after 72 hr Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells after 72 hr	10.1007/s00044-010-9322-5
Vero	IC₅₀	100 μg/mL Compound: rifampin	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19926361]
Vero	IC₅₀	108 μM Compound: RMP, rifampicin	Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay	[PMID: 24084159]
Vero	IC₅₀	113 μM Compound: RMP	Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiter assay Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiter assay	[PMID: 22391032]
Vero	IC₅₀	113 μM Compound: rifampicin	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by CellTiter 96 assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by CellTiter 96 assay	[PMID: 24909079]
Vero	IC₅₀	113 μM Compound: RMP, RFC	Cytotoxicity against african green monkey Vero cells after 72 hrs Cytotoxicity against african green monkey Vero cells after 72 hrs	[PMID: 21691438]
Vero	IC₅₀	122 μM Compound: RMP	Cytotoxicity against african green monkey Vero cells after 72 hrs Cytotoxicity against african green monkey Vero cells after 72 hrs	[PMID: 20022500]
Vero	IC₅₀	122 μM Compound: 1	Cytotoxicity against african green monkey Vero cells after 72 hrs Cytotoxicity against african green monkey Vero cells after 72 hrs	[PMID: 20000470]
Vero	IC₅₀	122 μM Compound: RMP	Cytotoxicity against african green monkey Vero cells after 72 hrs Cytotoxicity against african green monkey Vero cells after 72 hrs	[PMID: 19863050]
Vero	IC₅₀	125 μM Compound: Rifampin	Cytotoxicity against african green monkey Vero cells by colorimetric assay Cytotoxicity against african green monkey Vero cells by colorimetric assay	[PMID: 20116900]
Vero	IC₅₀	127 μM Compound: RMP	Cytotoxicity against african green monkey Vero cells after 72 hrs Cytotoxicity against african green monkey Vero cells after 72 hrs	[PMID: 19757815]
Vero	IC₅₀	127 μM Compound: RMP	Cytotoxicity against african green monkey Vero cells after 72 hrs by Cell titer assay Cytotoxicity against african green monkey Vero cells after 72 hrs by Cell titer assay	[PMID: 19271749]
Vero	IC₅₀	132.5 μM Compound: RMP	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTS-PMS assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTS-PMS assay	[PMID: 24079882]
Vero	IC₅₀	137.6 μg/mL Compound: Rifampicin	Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by MTT assay	[PMID: 31951961]
Vero	IC₅₀	138.3 μM Compound: RIF	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS assay	[PMID: 19119013]
Vero	CC₅₀	180 μM Compound: RIF	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assat Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assat	[PMID: 21146257]
Vero	CC₅₀	500 μg/mL Compound: RIF	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28720502]
Vero	CC₅₀	500 μg/mL Compound: RIF	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28646656]
Vero	CC₅₀	512 μg/mL Compound: RIF	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30690301]
Vero	CC₅₀	512 μg/mL Compound: RIF	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30025351]
Vero	CC₅₀	512 μg/mL Compound: RIF	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29227930]
Vero	CC₅₀	512 μM Compound: RIF	Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 30992202]
Vero	CC₅₀	512 μM Compound: RIF	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 30296686]
Vero	CC₅₀	7.8 μg/mL Compound: RIF	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 29407980]
Vero	IC₅₀	97.4 μg/mL Compound: Rifampin	Cytotoxicity against African green monkey Vero cells by CellTiter 96 aqueous nonradioactive cell proliferation assay Cytotoxicity against African green monkey Vero cells by CellTiter 96 aqueous nonradioactive cell proliferation assay	[PMID: 30295480]

In Vitro

Rifampicin (100 microg/mL) can block the functional activity of P-glycoprotein. Rifampicin is not a substract for P-glycoprotein. The mechanism of rifampicin resistance is unassociated with the functional activity of P-glycoprotein^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Rifampicin (200, 400 mg/kg) can induce fatty liver at high concentration^[1]. Rifampicin (30 mg/kg, i.p.) treatment of S464P biofilms in vivo results in a slight decline, but earlier rebinds in bioluminescence from these catheters compared with the parental signal, whereas rifampicin has no affect on bioluminescence in mice infected with mutant H481Y^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

822.94

Formula

C₄₃H₅₈N₄O₁₂

CAS No.

13292-46-1

Appearance

Solid

Color

Pink to red

SMILES

OC(C(/C=N/N1CCN(C)CC1)=C(NC(/C(C)=C\C=C\[C@@H]([C@@H]([C@@H](C)[C@H]2O)O)C)=O)C(O)=C3C(O)=C4C)=C3C5=C4O[C@](C)(O/C=C/[C@@H]([C@H]([C@@]([C@@H]2C)([H])OC(C)=O)C)OC)C5=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (30.38 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2152 mL	6.0758 mL	12.1516 mL
5 mM	0.2430 mL	1.2152 mL	2.4303 mL
10 mM	0.1215 mL	0.6076 mL	1.2152 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.04 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.15%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (253 KB) HNMR (329 KB) RP-HPLC (213 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Piriou A, et al. Fatty liver induced by high doses of rifampicin in the rat: possible relation with an inhibition of RNA polymerases in eukariotic cells. Arch Toxicol Suppl. 1979;(2):333-7. [Content Brief]

[2]. Yu J, et al. Monitoring in vivo fitness of rifampicin-resistant Staphylococcus aureus mutants in a mouse biofilm infection model. J Antimicrob Chemother. 2005 Apr;55(4):528-34. Epub 2005 Mar 2. [Content Brief]

[3]. Erokhina MV, et al. [In vitro development of rifampicin resistance in the epithelial cells]. Probl Tuberk Bolezn Legk. 2006;(8):58-61. [Content Brief]

[4]. Hamzehei M, et al. Inhibition of influenza A virus replication by rifampicin and selenocystamine. J Med Virol. 1980;6(2):169-74. [Content Brief]

[5]. Smee DF, et al. A review of compounds exhibiting anti-orthopoxvirus activity in animal models. Antiviral Res. 2003 Jan;57(1-2):41-52. [Content Brief]

Animal Administration
^[2]

Briefly, 1 cm Teflon catheter (14-gauge) carrying 104 cfu S. aureus, either the parental strain Xen 29 or the RifR mutants S464P or H481Y, are implanted subcutaneously in groups of nine mice per strain. One catheter segment is inserted on each side of each animal. Six days after the implantation of the catheters, five mice from each group are treated with rifampicin at 30 mg/kg intraperitoneally in 0.1 mL saline, twice daily for four consecutive days. The remaining four mice in each group are left untreated as controls. At various time points during the infection, the mice are anaesthetized using a constant flow of 1.5% isoflurane from the IVIS^® manifold, and imaged using an IVIS^® Image System 100 Series. The bioluminescent signals (photons/s) emitted from the mice are analysed using LivingImage^® software and plotted over the course of infection. The mice are sacrificed 20 days after infection (11 days after final rifampicin treatment). The catheters are surgically removed and the bacteria are detached by sonication for determination of bacterial burdens on the catheters.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Piriou A, et al. Fatty liver induced by high doses of rifampicin in the rat: possible relation with an inhibition of RNA polymerases in eukariotic cells. Arch Toxicol Suppl. 1979;(2):333-7. [Content Brief]

[2]. Yu J, et al. Monitoring in vivo fitness of rifampicin-resistant Staphylococcus aureus mutants in a mouse biofilm infection model. J Antimicrob Chemother. 2005 Apr;55(4):528-34. Epub 2005 Mar 2. [Content Brief]

[3]. Erokhina MV, et al. [In vitro development of rifampicin resistance in the epithelial cells]. Probl Tuberk Bolezn Legk. 2006;(8):58-61. [Content Brief]

[4]. Hamzehei M, et al. Inhibition of influenza A virus replication by rifampicin and selenocystamine. J Med Virol. 1980;6(2):169-74. [Content Brief]

[5]. Smee DF, et al. A review of compounds exhibiting anti-orthopoxvirus activity in animal models. Antiviral Res. 2003 Jan;57(1-2):41-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2152 mL	6.0758 mL	12.1516 mL	30.3789 mL
	5 mM	0.2430 mL	1.2152 mL	2.4303 mL	6.0758 mL
	10 mM	0.1215 mL	0.6076 mL	1.2152 mL	3.0379 mL
	15 mM	0.0810 mL	0.4051 mL	0.8101 mL	2.0253 mL
	20 mM	0.0608 mL	0.3038 mL	0.6076 mL	1.5189 mL
	25 mM	0.0486 mL	0.2430 mL	0.4861 mL	1.2152 mL
	30 mM	0.0405 mL	0.2025 mL	0.4051 mL	1.0126 mL

Rifampicin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rifampicin13292-46-1Rifampin Rifamycin AMPBacterialInfluenza VirusAntibioticOrthopoxvirusInhibitorinhibitorinhibit

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Rifampicin (Synonyms: Rifampin; Rifamycin AMP)

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