2. Apoptosis
  3. Bcl-2 Family Apoptosis
  4. BAI1

BAI1 est un inhibiteur de l'activation de BAX qui est sélectif et allostérique. BAI1 démontre l'inhibition de la perméabilisation membranaire médiée par BAX induite par tBID (HY-100464) avec une IC50 de 3,3 μΜ.

BAI1 is a selective and allosteric inhibitor of BAX, an apoptosis regulator. BAI1 directly binds to BAX and allosterically inhibits BAX activation. BAI1 has the potential for the research of diseases mediated by BAX-dependent cell death.

For research use only. We do not sell to patients.

BAI1 Chemical Structure

BAI1 Chemical Structure

CAS No. : 335165-68-9

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10 mM * 1 mL in DMSO
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of BAI1:

BAI1 Related Antibodies

Top Publications Citing Use of Products

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BAI1 is a selective and allosteric inhibitor of BAX, an apoptosis regulator. BAI1 directly binds to BAX and allosterically inhibits BAX activation. BAI1 has the potential for the research of diseases mediated by BAX-dependent cell death[1].

IC50 & Target[1]

Bax

 

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
0.52 μM
Compound: 1
In vitro inhibition of Bax regulated release of cytochrome C was determined using liposome channel assay
In vitro inhibition of Bax regulated release of cytochrome C was determined using liposome channel assay
[PMID: 14521400]
In Vitro

BAI1 selectively inhibits BAX-mediated apoptotic cell death[1].
? BAI1 shows inhibition of tBID (HY-100464)-induced BAX-mediated membrane permeabilization in a dose dependent manner with an IC50 of 3.3 μΜ[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BAI1 Related Antibodies

Apoptosis Analysis[1]

Cell Line: Mouse MEF
Concentration: 0.3125 μM , 0.625 μM, 1 μM, 1.25 μM, 1.5 μM, 2.5 μM , 3 μM, 4 μM, 5 4 μM, 10 4 μM
Incubation Time: 8 hours
Result: Inhibited cell death in BAX-dependent manner.
Molecular Weight

467.20

Formula

C19H21Br2N3O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC(CN1CCNCC1)CN2C3=C(C4=C2C=CC(Br)=C4)C=C(Br)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 18.46 mg/mL (39.51 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1404 mL 10.7021 mL 21.4041 mL
5 mM 0.4281 mL 2.1404 mL 4.2808 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.87 mg/mL (4.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.87 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (18.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.87 mg/mL (4.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.87 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (18.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

SDS (393 KB)

COA (256 KB) HNMR (403 KB) RP-HPLC (261 KB) MS (83 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1404 mL 10.7021 mL 21.4041 mL 53.5103 mL
5 mM 0.4281 mL 2.1404 mL 4.2808 mL 10.7021 mL
10 mM 0.2140 mL 1.0702 mL 2.1404 mL 5.3510 mL
15 mM 0.1427 mL 0.7135 mL 1.4269 mL 3.5674 mL
20 mM 0.1070 mL 0.5351 mL 1.0702 mL 2.6755 mL
25 mM 0.0856 mL 0.4281 mL 0.8562 mL 2.1404 mL
30 mM 0.0713 mL 0.3567 mL 0.7135 mL 1.7837 mL
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BAI1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BAI1335165-68-9BAI 1BAI-1Bcl-2 FamilyApoptosisBAXmitochondrialcelldeathpathwaycriticaleffectorInhibitorinhibitorinhibit

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