SN-38 (Synonyms: 7-Ethyl-10-hydroxycamptothecin)

Name: SN-38
Brand: MedChemExpress
SKU: HY-13704
Rating: 5.0 (42 reviews)

Cat. No.: HY-13704 Purity: 99.89%: FAQs
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SN-38 is an active metabolite of the Topoisomerase I inhibitor Irinotecan. SN-38 inhibits DNA and RNA synthesis with IC₅₀s of 0.077 and 1.3 μM, respectively. SN-38 is a payload of sacituzumab govitecan (HY -132254).

For research use only. We do not sell to patients.

SN-38 Chemical Structure

CAS No. : 86639-52-3

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10 mM * 1 mL in DMSO ready for reconstitution	USD 77	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 77	In-stock	Estimated Time of Arrival: December 31
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50 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
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Based on 42 publication(s) in Google Scholar

Other Forms of SN-38:

SN-38-d₃ Get quote
SN-38 (Standard) Get quote

42 Publications Citing Use of MCE SN-38

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Description

IC₅₀ & Target^[1]

Topoisomerase I

Camptothecins

Cellular Effect

Cell Line	Type	Value	Description	References
16HBE14o-	IC₅₀	0.3 μM Compound: SN38	Cytotoxicity against human 16HBE cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human 16HBE cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32832017]
A2780	IC₅₀	0.009 μM Compound: 3, SN38	Cytotoxicity against human A2780 cells overexpressing alpha5beta3 integrin assessed as cell survival after 72 hrs by SRB assay Cytotoxicity against human A2780 cells overexpressing alpha5beta3 integrin assessed as cell survival after 72 hrs by SRB assay	[PMID: 22959246]
A498	GI₅₀	0.22 μM Compound: SN-38	Growth inhibition of human A498 cells by Cell-titer96 assay Growth inhibition of human A498 cells by Cell-titer96 assay	[PMID: 33492141]
A549	IC₅₀	0.018 μM Compound: SN-38	Cytotoxicity against human A549 assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human A549 assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32650182]
A549	IC₅₀	0.028 μM Compound: 1d; SN38	Cytotoxicity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs Cytotoxicity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs	[PMID: 34048881]
A549	IC₅₀	0.08 μM Compound: SN-38	Cytotoxicity against human A549 cells by SRB method Cytotoxicity against human A549 cells by SRB method	[PMID: 18986807]
A549	IC₅₀	0.088 μM Compound: 5, SN-38	Antitumor activity against human A549/ATCC cells by SRB method Antitumor activity against human A549/ATCC cells by SRB method	[PMID: 19541483]
A549	IC₅₀	0.2 μM Compound: SN38	Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay	[PMID: 30169038]
A549	IC₅₀	0.3 μM Compound: SN38	Cytotoxicity against FR-negative human A549 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against FR-negative human A549 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32832017]
A549	IC₅₀	1.62 nM Compound: SN-38	Antiproliferative activity against human A549 cells after 72 hrs by SRB assay Antiproliferative activity against human A549 cells after 72 hrs by SRB assay	[PMID: 30115492]
A549	IC₅₀	11 nM Compound: SN38	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by SRB assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 32352777]
A549	IC₅₀	11.9 nM Compound: SN-38	Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by CCK-8 assay Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by CCK-8 assay	[PMID: 34963283]
A549	IC₅₀	2.72 nM Compound: 5, SN-38	Cytotoxicity against human A549 cells assessed as growth inhibition after 3 days by SRB assay Cytotoxicity against human A549 cells assessed as growth inhibition after 3 days by SRB assay	[PMID: 24529870]
A549	IC₅₀	3.8 nM Compound: SN-38	Cytotoxicity against human A549 cells assessed as cell growth inhibition incubated for 120 hrs by CCK-8 assay Cytotoxicity against human A549 cells assessed as cell growth inhibition incubated for 120 hrs by CCK-8 assay	[PMID: 34963283]
A549	IC₅₀	45.4 nM Compound: SN-38	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by SRB assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by SRB assay	[PMID: 32679451]
A549	IC₅₀	6.6 μM Compound: SN-38	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay	[PMID: 30935798]
ACHN	GI₅₀	5.9 nM Compound: 4; SN-38	Antiproliferative activity against human ACHN cells after 48 hrs by SRB assay Antiproliferative activity against human ACHN cells after 48 hrs by SRB assay	[PMID: 30553626]
Bel-7402	IC₅₀	13 nM Compound: SN-38	In-vitro inhibitory concentration for human tumor BEL-7402 cell-line was determined using MTT assay after 3 days of incubation In-vitro inhibitory concentration for human tumor BEL-7402 cell-line was determined using MTT assay after 3 days of incubation	[PMID: 15913996]
Cancer cell lines	GI₅₀	0.066 μM Compound: SN-38	Growth inhibition of human gastric cancer cells after 72 hrs by sulforhodamine B assay Growth inhibition of human gastric cancer cells after 72 hrs by sulforhodamine B assay	[PMID: 20884089]
Cancer cell lines	GI₅₀	0.066 μM Compound: SN-38	Growth inhibition of human lung cancer cells after 72 hrs by sulforhodamine B assay Growth inhibition of human lung cancer cells after 72 hrs by sulforhodamine B assay	[PMID: 20884089]
Cancer cell lines	GI₅₀	0.066 μM Compound: SN-38	Growth inhibition of human colon cancer cells after 72 hrs by sulforhodamine B assay Growth inhibition of human colon cancer cells after 72 hrs by sulforhodamine B assay	[PMID: 20884089]
CAPAN-1	IC₅₀	8 nM Compound: SN38	Antiproliferative activity against human Capan1 cells assessed as reduction in cell viability after 72 hrs by SRB assay Antiproliferative activity against human Capan1 cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 32352777]
CCD-841CoN	IC₅₀	4.98 μM Compound: 1d; SN38	Cytotoxicity against human CCD-841CoN cells assessed as inhibition of cell growth measured after 72 hrs Cytotoxicity against human CCD-841CoN cells assessed as inhibition of cell growth measured after 72 hrs	[PMID: 34048881]
COLO 205	IC₅₀	0.22 μM Compound: SN-38	Cytotoxicity against human COLO 205 assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human COLO 205 assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32650182]
DU-145	IC₅₀	0.004 μM Compound: SN-38	Antiproliferative activity against human DU145 cells after 96 hrs Antiproliferative activity against human DU145 cells after 96 hrs	[PMID: 18276141]
DU-145	IC₅₀	0.11 μM Compound: SN-38	Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 72 hrs by MTS assay Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 72 hrs by MTS assay	[PMID: 23622981]
DU-145	GI₅₀	4.3 nM Compound: 4; SN-38	Antiproliferative activity against human DU145 cells after 48 hrs by SRB assay Antiproliferative activity against human DU145 cells after 48 hrs by SRB assay	[PMID: 30553626]
HCT-116	IC₅₀	< 1 μM Compound: SN-38	Cytotoxicity against human HCT116 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay Cytotoxicity against human HCT116 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay	[PMID: 30935798]
HCT-116	IC₅₀	0.01 μM Compound: SN-38	Cytotoxicity against human HCT-116 assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human HCT-116 assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32650182]
HCT-116	GI₅₀	0.02 μM Compound: SN-38	Growth inhibition of human HCT-116 cells by Cell-titer96 assay Growth inhibition of human HCT-116 cells by Cell-titer96 assay	[PMID: 33492141]
HCT-116	IC₅₀	0.55 nM Compound: SN-38	Antiproliferative activity against human HCT116 cells after 3 days Antiproliferative activity against human HCT116 cells after 3 days	[PMID: 19254843]
HCT-116	IC₅₀	0.78 nM Compound: SN-38	Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay	[PMID: 30115492]
HCT-116	GI₅₀	23 nM Compound: 4; SN-38	Antiproliferative activity against human HCT116 cells after 48 hrs by SRB assay Antiproliferative activity against human HCT116 cells after 48 hrs by SRB assay	[PMID: 30553626]
HCT-116	IC₅₀	3.3 nM Compound: SN-38	In-vitro inhibitory concentration for human tumor HCT116 cell-line was determined using MTT assay after 3 days of incubation In-vitro inhibitory concentration for human tumor HCT116 cell-line was determined using MTT assay after 3 days of incubation	[PMID: 15913996]
HCT-116	IC₅₀	31 nM Compound: 5, SN-38	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay	[PMID: 24529870]
HCT-116	IC₅₀	4 nM Compound: SN38	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by SRB assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 32352777]
HCT-116	IC₅₀	4.28 nM Compound: SN-38	Cytotoxicity against human HCT116 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human HCT116 cells after 72 hrs by sulforhodamine B assay	[PMID: 25835359]
HCT-116	IC₅₀	55.1 nM Compound: SN-38	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by SRB assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by SRB assay	[PMID: 32679451]
HCT-15	IC₅₀	0.008 μM Compound: SN-38	Cytotoxicity against human HCT15 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human HCT15 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 27060760]
HEK293	IC₅₀	< 1 μM Compound: SN-38	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay	[PMID: 30935798]
HEK293	IC₅₀	360 μM Compound: SN-38	Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in bilirubin glucuronidation by LC-MS/MS method Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in bilirubin glucuronidation by LC-MS/MS method	[PMID: 21030469]
HEK-293T	IC₅₀	14.2 nM Compound: SN-38	Cytotoxicity against HEK293T cells assessed as reduction in cell viability measured after 72 hrs by CCK-8 assay Cytotoxicity against HEK293T cells assessed as reduction in cell viability measured after 72 hrs by CCK-8 assay	[PMID: 34963283]
HeLa	IC₅₀	> 1 μM Compound: SN38	Cytotoxicity against oleic acid-induced human HeLa cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against oleic acid-induced human HeLa cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 35059132]
HeLa	IC₅₀	0.145 μM Compound: SN-38	Cytotoxicity against human Hela assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human Hela assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32650182]
HeLa	IC₅₀	0.3 μM Compound: SN38	Cytotoxicity against FR-positive human HeLa cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against FR-positive human HeLa cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32832017]
HeLa	IC₅₀	13.1 μM Compound: SN-38	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay	[PMID: 30935798]
HeLa	IC₅₀	349 nM Compound: SN38	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 35059132]
Hep 3B2	GI₅₀	0.177 μM Compound: SN-38	Growth inhibition of human Hep3B cells by Cell-titer96 assay Growth inhibition of human Hep3B cells by Cell-titer96 assay	[PMID: 33492141]
HepG2	IC₅₀	0.48 μM Compound: SN38	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 30600208]
HL-60	IC₅₀	0.002 μM Compound: 1d; SN38	Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth measured after 72 hrs Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth measured after 72 hrs	[PMID: 34048881]
HL-60	IC₅₀	19 nM Compound: SN-38	In-vitro inhibitory concentration for human tumor HL60 cell-line was determined using SRB assay after 3 days of incubation In-vitro inhibitory concentration for human tumor HL60 cell-line was determined using SRB assay after 3 days of incubation	[PMID: 15913996]
HL-60	IC₅₀	2.4 μM Compound: SN-38	Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay	[PMID: 30935798]
HOC-21 cell line	IC₅₀	123.1 ng/mL Compound: 5	Tested in vitro for the inhibition of HOC-21(human ovarian cancer) cell line by MTT assay Tested in vitro for the inhibition of HOC-21(human ovarian cancer) cell line by MTT assay	[PMID: 7932525]
HT-29	IC₅₀	0.012 μM Compound: SN-38	Antiproliferative activity against human HT29 cells after 96 hrs Antiproliferative activity against human HT29 cells after 96 hrs	[PMID: 18276141]
HT-29	IC₅₀	0.02 μM Compound: 1d; SN38	Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth measured after 72 hrs Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth measured after 72 hrs	[PMID: 34048881]
HT-29	IC₅₀	0.054 μM Compound: SN-38	Cytotoxicity against human HT-29 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 27060760]
HT-29	IC₅₀	0.096 μM Compound: SN-38	Cytotoxicity against human HT-29 cells assessed as growth reduction under normoxic condition after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as growth reduction under normoxic condition after 72 hrs by MTT assay	[PMID: 28740613]
HT-29	IC₅₀	0.119 μM Compound: SN-38	Cytotoxicity against human HT-29 cells assessed as growth reduction under hypoxic condition after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as growth reduction under hypoxic condition after 72 hrs by MTT assay	[PMID: 28740613]
HT-29	IC₅₀	0.12 μM Compound: SN-38	Cytotoxicity against human HT-29 cells by SRB method Cytotoxicity against human HT-29 cells by SRB method	[PMID: 18986807]
HT-29	IC₅₀	0.17 μM Compound: 5, SN-38	Antitumor activity against human HT-29 cells by SRB method Antitumor activity against human HT-29 cells by SRB method	[PMID: 19541483]
HT-29	IC₅₀	0.67 μM Compound: SN-38	Antiproliferative activity against human HT29 cells after 1 hr of drug exposure measured after 72 hrs Antiproliferative activity against human HT29 cells after 1 hr of drug exposure measured after 72 hrs	[PMID: 18424133]
HT-29	IC₅₀	106.67 nM Compound: 5, SN-38	Cytotoxicity against human HT-29 cells assessed as growth inhibition after 3 days by SRB assay Cytotoxicity against human HT-29 cells assessed as growth inhibition after 3 days by SRB assay	[PMID: 24529870]
HT-29	IC₅₀	11.5 μM Compound: SN-38	Antiproliferative activity against BCRP overexpressing mitoxantrone-resistant human HT29 cells after 1 hr of drug exposure measured after 72 hrs Antiproliferative activity against BCRP overexpressing mitoxantrone-resistant human HT29 cells after 1 hr of drug exposure measured after 72 hrs	[PMID: 18424133]
HT-29	IC₅₀	18.54 nM Compound: SN-38	Cytotoxicity against human HT-29 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human HT-29 cells after 72 hrs by sulforhodamine B assay	[PMID: 25835359]
HT-29	IC₅₀	2.74 μM Compound: SN-38	Cytotoxicity against human HT-29 cells pretreated for 24 hrs under normoxic condition followed by compound washout measured after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells pretreated for 24 hrs under normoxic condition followed by compound washout measured after 72 hrs by MTT assay	[PMID: 28350997]
HT-29	IC₅₀	3.71 μM Compound: SN-38	Cytotoxicity against human HT-29 cells pretreated for 24 hrs under hypoxic condition followed by compound washout measured after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells pretreated for 24 hrs under hypoxic condition followed by compound washout measured after 72 hrs by MTT assay	[PMID: 28350997]
Jurkat	IC₅₀	< 1 μM Compound: SN-38	Cytotoxicity against human Jurkat cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay Cytotoxicity against human Jurkat cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay	[PMID: 30935798]
KB 3-1	IC₅₀	0.004 μM Compound: SN-38	Cytotoxicity against human KB3-1 cells after 4 days by MTT method Cytotoxicity against human KB3-1 cells after 4 days by MTT method	[PMID: 19303306]
KB-V1	IC₅₀	0.046 μM Compound: SN-38	Cytotoxicity against MDR1 overexpressing human KBV1 cells after 4 days by MTT method Cytotoxicity against MDR1 overexpressing human KBV1 cells after 4 days by MTT method	[PMID: 19303306]
L02	IC₅₀	18 nM Compound: SN38	Cytotoxicity agains human HL7702 cells assessed as reduction in cell viability after 72 hrs by SRB assay Cytotoxicity agains human HL7702 cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 32352777]
LNCaP	GI₅₀	0.0122 μM Compound: SN-38	Growth inhibition of human LNCaP cells by Cell-titer96 assay Growth inhibition of human LNCaP cells by Cell-titer96 assay	[PMID: 33492141]
LoVo	IC₅₀	0.301 μM Compound: SN-38	Cytotoxicity against human LoVo assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human LoVo assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32650182]
LOX IMVI	GI₅₀	5.3 nM Compound: 4; SN-38	Antiproliferative activity against human LOXIMVI cells after 48 hrs by SRB assay Antiproliferative activity against human LOXIMVI cells after 48 hrs by SRB assay	[PMID: 30553626]
MCF7	IC₅₀	< 0.1 nM Compound: SN38	Cytotoxicity against human MCF7 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human MCF7 cells after 72 hrs by sulforhodamine B assay	[PMID: 30169038]
MCF7	IC₅₀	0.515 μM Compound: SN-38	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 27060760]
MCF7	IC₅₀	0.66 μM Compound: 1d; SN38	Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth measured after 72 hrs Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth measured after 72 hrs	[PMID: 34048881]
MCF7	IC₅₀	1.25 μM Compound: SN38	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 30600208]
MCF7	GI₅₀	3 nM Compound: 4; SN-38	Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 30553626]
MDA-MB-231	GI₅₀	0.512 μM Compound: SN-38	Growth inhibition of human MDA-MB-231 cells by Cell-titer96 assay Growth inhibition of human MDA-MB-231 cells by Cell-titer96 assay	[PMID: 33492141]
MDA-MB-231	IC₅₀	1.97 μM Compound: SN-38	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 27060760]
MDCK-II	GI₅₀	0.139 μM Compound: SN-38	Cytotoxicity against wild-type MDCK-II cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against wild-type MDCK-II cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 33454462]
MIA PaCa-2	IC₅₀	13 nM Compound: SN38	Antiproliferative activity against human MIAPaCa2 cells assessed as reduction in cell viability after 72 hrs by SRB assay Antiproliferative activity against human MIAPaCa2 cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 32352777]
MKN-28	GI₅₀	110 nM Compound: 4; SN-38	Antiproliferative activity against human MKN28 cells after 48 hrs by SRB assay Antiproliferative activity against human MKN28 cells after 48 hrs by SRB assay	[PMID: 30553626]
MRC5	IC₅₀	15.3 μM Compound: SN-38	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay	[PMID: 30935798]
NCI-H1975	IC₅₀	0.81 μM Compound: SN-38	Cytotoxicity against human NCI-H1975 assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human NCI-H1975 assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32650182]
NCI-H226	GI₅₀	0.277 μM Compound: SN-38	Growth inhibition of human NCI-H226 cells by Cell-titer96 assay Growth inhibition of human NCI-H226 cells by Cell-titer96 assay	[PMID: 33492141]
NCI-H460	IC₅₀	0.047 μM Compound: SN-38	Cytotoxicity against human NCI-H460 cells assessed as growth reduction under hypoxic condition after 72 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as growth reduction under hypoxic condition after 72 hrs by MTT assay	[PMID: 28740613]
NCI-H460	IC₅₀	0.05 μM Compound: SN-38	Cytotoxicity against human NCI-H460 cells assessed as growth reduction under normoxic condition after 72 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as growth reduction under normoxic condition after 72 hrs by MTT assay	[PMID: 28740613]
NCI-H460	IC₅₀	0.05 μM Compound: SN-38	Cytotoxicity against human NCI-H460 cells pretreated for 24 hrs under normoxic condition followed by compound washout measured after 72 hrs by MTT assay Cytotoxicity against human NCI-H460 cells pretreated for 24 hrs under normoxic condition followed by compound washout measured after 72 hrs by MTT assay	[PMID: 28350997]
NCI-H460	IC₅₀	0.06 μM Compound: SN-38	Cytotoxicity against human NCI-H460 cells pretreated for 24 hrs under hypoxic condition followed by compound washout measured after 72 hrs by MTT assay Cytotoxicity against human NCI-H460 cells pretreated for 24 hrs under hypoxic condition followed by compound washout measured after 72 hrs by MTT assay	[PMID: 28350997]
NCI-H460	IC₅₀	0.13 μM Compound: SN-38	Antiproliferative activity against human NCI-H460 cells after short term exposure for 1 hr measured after 72 hrs in drug-free medium Antiproliferative activity against human NCI-H460 cells after short term exposure for 1 hr measured after 72 hrs in drug-free medium	[PMID: 19530720]
NCI-H460	IC₅₀	0.21 μM Compound: SN-38	In vitro inhibitory concentration against human lung large cell carcinoma cell line, H460 (after 1 hour exposure) In vitro inhibitory concentration against human lung large cell carcinoma cell line, H460 (after 1 hour exposure)	[PMID: 15501036]
NCI-H460	IC₅₀	0.22 μM Compound: SN-38	Antiproliferative activity against human H460 cells after 1 hr of drug exposure measured after 72 hrs Antiproliferative activity against human H460 cells after 1 hr of drug exposure measured after 72 hrs	[PMID: 18424133]
NCI-H460	IC₅₀	3.3 nM Compound: SN-38	Antiproliferative activity against human NCI-H460 cells after 3 days Antiproliferative activity against human NCI-H460 cells after 3 days	[PMID: 19254843]
NCI-H522	GI₅₀	3.7 nM Compound: 4; SN-38	Antiproliferative activity against human NCI-H522 cells after 48 hrs by SRB assay Antiproliferative activity against human NCI-H522 cells after 48 hrs by SRB assay	[PMID: 30553626]
P388	IC₅₀	6.93 ng/mL Compound: 5	Tested in vitro for the inhibition of P388 (mouse leukemia) cell line by MTT assay; 1.95-6.93 ng/mL Tested in vitro for the inhibition of P388 (mouse leukemia) cell line by MTT assay; 1.95-6.93 ng/mL	[PMID: 7932525]
PC-3	IC₅₀	0.0026 μM Compound: 3, SN38	Cytotoxicity against human PC3 cells expressing alpha5beta3 integrin assessed as cell survival after 72 hrs by SRB assay Cytotoxicity against human PC3 cells expressing alpha5beta3 integrin assessed as cell survival after 72 hrs by SRB assay	[PMID: 22959246]
PC-6	IC₅₀	0.43 nM Compound: SN-38	Antiproliferative activity against human PC6 cells carrying pRC after 6 days Antiproliferative activity against human PC6 cells carrying pRC after 6 days	[PMID: 19254843]
PC-6	IC₅₀	5.1 nM Compound: SN-38	Antiproliferative activity against human PC6 expressing BCRP cells after 6 days Antiproliferative activity against human PC6 expressing BCRP cells after 6 days	[PMID: 19254843]
QG-56	IC₅₀	2.8 nM Compound: SN-38	Antiproliferative activity against human QG56 cells after 3 days Antiproliferative activity against human QG56 cells after 3 days	[PMID: 19254843]
QG-56	IC₅₀	3.17 ng/mL Compound: 5	Tested in vitro for the inhibition of QG-56 (human lung squamous cell carcinoma) cell line by MTT assay Tested in vitro for the inhibition of QG-56 (human lung squamous cell carcinoma) cell line by MTT assay	[PMID: 7932525]
Raji	IC₅₀	0.005 μM Compound: SN-38	Cytotoxicity against human Raji assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human Raji assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32650182]
RPMI-7951	IC₅₀	0.26 μM Compound: SN 38	Cytotoxicity against human RPMI7951 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human RPMI7951 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 32435422]
SiHa	IC₅₀	0.3 μM Compound: SN38	Cytotoxicity against FR-positive human SiHa cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against FR-positive human SiHa cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32832017]
SJRH30	GI₅₀	0.0093 μM Compound: SN-38	Growth inhibition of human SJRH30 cells by Cell-titer96 assay Growth inhibition of human SJRH30 cells by Cell-titer96 assay	[PMID: 33492141]
SK-HEP1	IC₅₀	0.3 μM Compound: SN38	Cytotoxicity against FR-positive human SKHEP1 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against FR-positive human SKHEP1 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32832017]
SK-MEL-24	IC₅₀	0.48 μM Compound: SN 38	Cytotoxicity against human SK-MEL-24 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human SK-MEL-24 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 32435422]
SK-OV-3	GI₅₀	23 nM Compound: 4; SN-38	Antiproliferative activity against human SKOV3 cells after 48 hrs by SRB assay Antiproliferative activity against human SKOV3 cells after 48 hrs by SRB assay	[PMID: 30553626]
TE-8	IC₅₀	< 1 μM Compound: SN-38	Cytotoxicity against human TE8 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay Cytotoxicity against human TE8 cells assessed as reduction in cell viability incubated for 48 hrs by WST8 dye based assay	[PMID: 30935798]
U-251	IC₅₀	0.021 μM Compound: SN-38	Cytotoxicity against human U251 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human U251 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 27060760]
U-251	GI₅₀	2.8 nM Compound: 4; SN-38	Antiproliferative activity against human U251 cells after 48 hrs by SRB assay Antiproliferative activity against human U251 cells after 48 hrs by SRB assay	[PMID: 30553626]
U-87MG ATCC	IC₅₀	0.03 μM Compound: SN-38	Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 27060760]

In Vitro

The IC₅₀ values for LoVo, HCT116, and HT29 cell lines is 20 nM, 50 nM, 130 nM, respectively. In all three SN-38 resistant cell lines Top1 activity is maintained in the presence of high concentrations of SN-38^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SN-38, the active and toxic metabolite of the anticancer prodrug Irinotecan. At 30 minutes after administration, Irinotecan plasma concentrations in Slco1a/1b(?/?) mice are 1.9-fold higher than in the wild-type mice (1.89 vs. 1.01 μM, respectively), whereas SN-38 plasma concentrations of Slco1a/1b(?/?) mice are 8-fold higher compare with wild-type mice (0.4 μg/mL vs. 0.05 μg/mL, respectively). Overall plasma exposure [AUC_(5-240)] of Irinotecan is 1.7-fold higher in Oatp1a/1b knockout mice versus wild-type mice (209.8±6.7 vs. 120.9±4.4 μM/min; P<0.01), and 2.9-fold higher for SN-38 (50±2.9 vs. 12±2 μM/min; P<0.001)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

392.40

Formula

C₂₂H₂₀N₂O₅

CAS No.

86639-52-3

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1[C@](O)(CC)C2=C(CO1)C(N3CC4=C(CC)C5=CC(O)=CC=C5N=C4C3=C2)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (63.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5484 mL	12.7421 mL	25.4842 mL
5 mM	0.5097 mL	2.5484 mL	5.0968 mL
10 mM	0.2548 mL	1.2742 mL	2.5484 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (6.37 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (5.30 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (5.30 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (283 KB) SDS (479 KB)

COA (254 KB) HNMR (297 KB) LCMS (96 KB) OR (172 KB)

Handling Instructions (2659 KB)

References

[1]. Wallin A, et al. Anticancer effect of SN-38 on colon cancer cell lines with different metastatic potential. Oncol Rep. 2008 Jun;19(6):1493-8. [Content Brief]

[2]. Jensen NF, et al. Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a newpair of resistance-associated mutations. J Exp Clin Cancer Res. 2016 Mar 31;35:56. [Content Brief]

[3]. Kawato Y, et al. Intracellular roles of SN-38, a metabolite of the camptothecin derivative CPT-11, in the antitumor effect of CPT-11. Cancer Res. 1991;51(16):4187-4191. [Content Brief]

[4]. Aaron T Jacobs, et al. Targeted therapy for breast cancer: An overview of drug classes and outcomes. Biochem Pharmacol. 2022 Oct:204:115209. [Content Brief]

Cell Assay ^[2]	In vitro SN-38 sensitivity is determined using the MTT assay. Cells are seeded in 96-well plates, and a range of SN-38 concentrations is added the following day. Following 48 h of drug exposure, the medium is discarded and the plates are incubated with medium containing MTT (0.5 mg/mL) for 3 h. Acidified (0.02 M HCl) sodium dodecyl sulphate (20 %) is added to dissolve the formed formazan. Optical density at 570 nm (and 670 nm for background) is measured, and the cell viability is calculated in percent compared to untreated cells. Experiments are repeated three times and the mean IC₅₀ value ± standard deviation is determined. Relative resistance for each resistant cell line is calculated by dividing the mean IC₅₀ value of the resistant cell line by the mean IC₅₀ value of the corresponding parental cell line^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Mice^[3] Female wild-type, Slco1a/1b(−/−)(Oatp1a/1b knockout), Slco1a/1b(−/−);1B1(tg), and Slco1a/1b(−/−);1B3(tg) (liver-specific OATP1B1 and OATP1B3 humanized transgenic) mice of comparable genetic background (>99% FVB) between 8 and 14 weeks of age are used. Irinotecan (20 mg/mL in water-based solution containing NaOH, lactic acid, and sorbitol) is diluted with saline (to 2 mg/mL) for administration of 10 mg/kg; 5 μL/g bodyweight are administered intravenously to mice. SN-38 is dissolved in DMSO (1 mg/mL) and 1 μL/g body weight is administered intravenously to mice to achieve a dosage of 1 mg/kg. The experiments are terminated by isoflurane anaesthesia, heparin-blood sampling by cardiac puncture followed by cervical dislocation and tissue collection. Blood samples are centrifuged at 5,200 × g for 5 minutes at 4°C and plasma is collected and stored at −30°C until analysis. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Wallin A, et al. Anticancer effect of SN-38 on colon cancer cell lines with different metastatic potential. Oncol Rep. 2008 Jun;19(6):1493-8. [Content Brief] [2]. Jensen NF, et al. Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a newpair of resistance-associated mutations. J Exp Clin Cancer Res. 2016 Mar 31;35:56. [Content Brief] [3]. Kawato Y, et al. Intracellular roles of SN-38, a metabolite of the camptothecin derivative CPT-11, in the antitumor effect of CPT-11. Cancer Res. 1991;51(16):4187-4191. [Content Brief] [4]. Aaron T Jacobs, et al. Targeted therapy for breast cancer: An overview of drug classes and outcomes. Biochem Pharmacol. 2022 Oct:204:115209. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5484 mL	12.7421 mL	25.4842 mL	63.7105 mL
	5 mM	0.5097 mL	2.5484 mL	5.0968 mL	12.7421 mL
	10 mM	0.2548 mL	1.2742 mL	2.5484 mL	6.3711 mL
	15 mM	0.1699 mL	0.8495 mL	1.6989 mL	4.2474 mL
	20 mM	0.1274 mL	0.6371 mL	1.2742 mL	3.1855 mL
	25 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5484 mL
	30 mM	0.0849 mL	0.4247 mL	0.8495 mL	2.1237 mL
	40 mM	0.0637 mL	0.3186 mL	0.6371 mL	1.5928 mL
	50 mM	0.0510 mL	0.2548 mL	0.5097 mL	1.2742 mL
	60 mM	0.0425 mL	0.2124 mL	0.4247 mL	1.0618 mL

SN-38 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SN-3886639-52-37-Ethyl-10-hydroxycamptothecinSN38SN 38Drug MetaboliteTopoisomeraseADC CytotoxinAutophagyADC PayloadInhibitorinhibitorinhibit

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SN-38 (Synonyms: 7-Ethyl-10-hydroxycamptothecin)

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