D18

Name: D18
Brand: MedChemExpress
SKU: HY-144501
Rating: 4.5 (1 reviews)

Cat. No.: HY-144501 Purity: 98.14%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

D18 is an immune modulator. D18 acts as a TLR7/8 dual agonist (EC₅₀=24 nM for hTLR7 and 10 nM for hTLR8, respectively). D18 increases PD-L1 expression through epigenetic regulation, thus sensitizing tumors to PD-1/PD-L1 blockade. D18 is a ADC cytotoxin uesd for the systhesis of ADC HE-S2.

For research use only. We do not sell to patients.

D18 Chemical Structure

CAS No. : 2230218-36-5

Size	Price	Stock	Quantity
1 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 2020	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 4050	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

364.49

Formula

C₂₁H₂₈N₆

CAS No.

2230218-36-5

Appearance

Solid

Color

White to light yellow

SMILES

NC(N=C1NCCCC)=NC(C1=N2)=CC=C2CC(C=C3)=CC=C3CN(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (274.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7436 mL	13.7178 mL	27.4356 mL
5 mM	0.5487 mL	2.7436 mL	5.4871 mL
10 mM	0.2744 mL	1.3718 mL	2.7436 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.86 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.86 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (6.86 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.79%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (246 KB) HNMR (157 KB) RP-HPLC (155 KB) LCMS (374 KB)

Handling Instructions (2659 KB)

References

[1]. He L, et al. Immune Modulating Antibody-Drug Conjugate (IM-ADC) for Cancer Immunotherapy. J Med Chem. 2021;64(21):15716-15726. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7436 mL	13.7178 mL	27.4356 mL	68.5890 mL
	5 mM	0.5487 mL	2.7436 mL	5.4871 mL	13.7178 mL
	10 mM	0.2744 mL	1.3718 mL	2.7436 mL	6.8589 mL
	15 mM	0.1829 mL	0.9145 mL	1.8290 mL	4.5726 mL
	20 mM	0.1372 mL	0.6859 mL	1.3718 mL	3.4294 mL
	25 mM	0.1097 mL	0.5487 mL	1.0974 mL	2.7436 mL
	30 mM	0.0915 mL	0.4573 mL	0.9145 mL	2.2863 mL
	40 mM	0.0686 mL	0.3429 mL	0.6859 mL	1.7147 mL
	50 mM	0.0549 mL	0.2744 mL	0.5487 mL	1.3718 mL
	60 mM	0.0457 mL	0.2286 mL	0.4573 mL	1.1431 mL
	80 mM	0.0343 mL	0.1715 mL	0.3429 mL	0.8574 mL
	100 mM	0.0274 mL	0.1372 mL	0.2744 mL	0.6859 mL

D18 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D182230218-36-5D 18D-18Toll-like Receptor (TLR)PD-1/PD-L1PD-1/Programmed death-ligand 1immune checkpoint blockadeKDM5ATLR7/8immune modulatorepigenetic regulationcancer immunotherapyInhibitorinhibitorinhibit

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D18

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D18 Related Antibodies

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References

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Dilution Calculator

Complete Stock Solution Preparation Table

D18 Related Classifications

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