1,2,4-Trihydroxybenzene

Cat. No.: HY-W010451 Purity: 98.76%: Data Sheet
SDS

COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

1,2,4-Trihydroxybenzene (Hydroxyhydroquinone), a by-product of coffee bean roasting, increases intracellular Ca²⁺ concentration in rat thymic lymphocytes.

For research use only. We do not sell to patients.

1,2,4-Trihydroxybenzene Chemical Structure

CAS No. : 533-73-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 42	In-stock	Estimated Time of Arrival: April 15
Solution
10 mM * 1 mL in DMSO	USD 44	In-stock	Estimated Time of Arrival: April 15
Solid
500 mg	USD 40	In-stock	Estimated Time of Arrival: April 15
1 g		Get quote
5 g		Get quote

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Other Forms of 1,2,4-Trihydroxybenzene:

1,2,4-Trihydroxybenzene (Standard) Get quote

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Biological Activity
Purity & Documentation
References
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Description

1,2,4-Trihydroxybenzene (Hydroxyhydroquinone), a by-product of coffee bean roasting, increases intracellular Ca²⁺ concentration in rat thymic lymphocytes^[1].

Molecular Weight

126.11

Formula

C₆H₆O₃

CAS No.

533-73-3

Appearance

Solid

Color

Brown to gray

SMILES

OC1=CC=C(O)C=C1O

Structure Classification

Initial Source

Microorganisms

Phanerochaete chrysosporium

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (396.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.9296 mL	39.6479 mL	79.2959 mL
5 mM	1.5859 mL	7.9296 mL	15.8592 mL
10 mM	0.7930 mL	3.9648 mL	7.9296 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (19.82 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (19.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Purity & Documentation

Purity: 99.12%

Select Batch:

Data Sheet (271 KB) SDS (614 KB)

COA (248 KB)

Handling Instructions (2659 KB)

References

[1]. Risa Kamae, et al. Hydroxyhydroquinone, a by-product of coffee bean roasting, increases intracellular Ca²⁺ concentration in rat thymic lymphocytes. Food Chem Toxicol. 2017 Apr;102:39-45. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	7.9296 mL	39.6479 mL	79.2959 mL	198.2396 mL
	5 mM	1.5859 mL	7.9296 mL	15.8592 mL	39.6479 mL
	10 mM	0.7930 mL	3.9648 mL	7.9296 mL	19.8240 mL
	15 mM	0.5286 mL	2.6432 mL	5.2864 mL	13.2160 mL
	20 mM	0.3965 mL	1.9824 mL	3.9648 mL	9.9120 mL
	25 mM	0.3172 mL	1.5859 mL	3.1718 mL	7.9296 mL
	30 mM	0.2643 mL	1.3216 mL	2.6432 mL	6.6080 mL
	40 mM	0.1982 mL	0.9912 mL	1.9824 mL	4.9560 mL
	50 mM	0.1586 mL	0.7930 mL	1.5859 mL	3.9648 mL
	60 mM	0.1322 mL	0.6608 mL	1.3216 mL	3.3040 mL
	80 mM	0.0991 mL	0.4956 mL	0.9912 mL	2.4780 mL
	100 mM	0.0793 mL	0.3965 mL	0.7930 mL	1.9824 mL