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AAPH  (Synonyms: 2,2'-Azodiisobutyramidine dihydrochloride)

Cat. No.: HY-Y0525 Purity: ≥98.0%
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AAPH (2,2'-Azodiisobutyramidine dihydrochloride) has an effect of radical generation. AAPH induces oxidative stress and erythrocyte hemolysis. AAPH decomposes at 37℃ to generate an alkyl radical, is used as an initiator. In the presence of oxygen, these alkyl radicals will be converted to peroxyl radicals that can cause lipid peroxidation and loss of erythrocyte membrane integrity, which could ultimately lead to hemolysis.

For research use only. We do not sell to patients.

AAPH Chemical Structure

AAPH Chemical Structure

CAS No. : 2997-92-4

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Based on 1 publication(s) in Google Scholar

AAPH Related Antibodies

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Description

AAPH (2,2'-Azodiisobutyramidine dihydrochloride) has an effect of radical generation. AAPH induces oxidative stress and erythrocyte hemolysis[1]. AAPH decomposes at 37℃ to generate an alkyl radical, is used as an initiator. In the presence of oxygen, these alkyl radicals will be converted to peroxyl radicals that can cause lipid peroxidation and loss of erythrocyte membrane integrity, which could ultimately lead to hemolysis[1][2][3][4].

In Vitro

AAPH (600 μM, 0-1 h) oxidates DCFH to form a fluorescent substance, DCF, which the level of fluorescence was proportional to the degree of oxidation in HepG2 cells[2].
AAPH (200 mM, 2 h) induces the erythrocyte losting normal profile, shows a spiny configuration on the surface due to the severe damage of the cell membrane and produces a large amount of ROS in in human erythrocytes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AAPH Related Antibodies
Molecular Weight

271.19

Formula

C8H20Cl2N6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(/N=N/C(C)(C)C(N)=N)(C)C(N)=N.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (368.75 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6875 mL 18.4373 mL 36.8745 mL
5 mM 0.7375 mL 3.6875 mL 7.3749 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (749 KB)

COA (247 KB) HNMR (126 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.6875 mL 18.4373 mL 36.8745 mL 92.1863 mL
5 mM 0.7375 mL 3.6875 mL 7.3749 mL 18.4373 mL
10 mM 0.3687 mL 1.8437 mL 3.6875 mL 9.2186 mL
15 mM 0.2458 mL 1.2292 mL 2.4583 mL 6.1458 mL
20 mM 0.1844 mL 0.9219 mL 1.8437 mL 4.6093 mL
25 mM 0.1475 mL 0.7375 mL 1.4750 mL 3.6875 mL
30 mM 0.1229 mL 0.6146 mL 1.2292 mL 3.0729 mL
40 mM 0.0922 mL 0.4609 mL 0.9219 mL 2.3047 mL
50 mM 0.0737 mL 0.3687 mL 0.7375 mL 1.8437 mL
60 mM 0.0615 mL 0.3073 mL 0.6146 mL 1.5364 mL
80 mM 0.0461 mL 0.2305 mL 0.4609 mL 1.1523 mL
100 mM 0.0369 mL 0.1844 mL 0.3687 mL 0.9219 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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AAPH Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AAPH2997-92-42,2'-AzodiisobutyramidineOthersoxidative stressradicalerythrocyte hemolysisInhibitorinhibitorinhibit

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