ADL-5859 hydrochloride

Name: ADL-5859 hydrochloride
Brand: MedChemExpress
SKU: HY-13044
Rating: 5.0 (2 reviews)

Cat. No.: HY-13044 Purity: 99.77%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ADL-5859 hydrochloride (compound 20) is a selective and orally active δ opioid receptor (DOR) agonist with an K_i and an EC₅₀ value of 0.84 and 20 nM, respectively. ADL-5859 hydrochloride also shows inhibitory activity to hERG channel with an IC₅₀ value of 78 μM. ADL-5859 hydrochloride can be used for the research of pain.

For research use only. We do not sell to patients.

ADL-5859 hydrochloride Chemical Structure

CAS No. : 850173-95-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 143	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 143	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 770	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1270	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of ADL-5859 hydrochloride:

ADL5859 Get quote

1 Publications Citing Use of MCE ADL-5859 hydrochloride

•Eur J Pharmacol. 2016 Jun 15;781:53-9. [Abstract]

Proliferation Assay

: ADL-5859 hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2016 Jun 15;781:53-9. [Abstract]; The DOR agonist ADL5859 shows antidepressant-like effects in the FST in C57BL/6J mice.

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Opioid Receptor Isoform Specific Products:

View All Isoforms

μ Opioid Receptor/MOR κ Opioid Receptor/KOR δ Opioid Receptor/DOR NOP Receptor/ORL1

View All Cytochrome P450 Isoform Specific Products:

View All Isoforms

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

δ Opioid Receptor/DOR

In Vitro

ADL-5859 (0-10 μM) hydrochloride shows activities to δ opioid receptor with an K_i and an EC₅₀ value of 0.84 and 20 nM, and inhibits 32% and 37% activities to μ and κ opioid receptor, respectively^[1].
ADL-5859 (0-100 μM) hydrochloride exhibits inhibitory activity to hERG channel with an IC₅₀ value of 78 μM^[1].
ADL-5859 (0-100 μM) hydrochloride inhibits activity of the drug metabolizing enzyme cytochrome P450 2D6 (CYP2D6) in vitro with an IC₅₀ value of 43 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ADL-5859 (0.3-10 mg/kg; p.o. once) hydrochloride reverses hyperalgesia in inflamed paw of rats and shows robust antidepressant-like activity^[1].
1.19 Pharmacokinetic Properties of ADL-5859 in Rats and Dogs^[1].

	Rats IV 0.25 mg/kg and PO 3 mg/kg	Dogs IV 1 mg/kg and PO 3 mg/kg
CLs (L/h/kg)	1.8±0.5	0.4±0.1
Vdss (L/kg)	10.7±1.9	2.5±0.5
t_1/2 (oral, h)	5.3±0.7	4.7±0.2
F (%)	33.4±3.2	66.5±6.8

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rats with Freund’s Complete Adjuvant (FCA) injection induced mechanical hyperalgesia^[1]
Dosage:	0.3-10 mg/kg
Administration:	Oral gavage; 0.3-10 mg/kg once
Result:	Produced 100% reversal of hyperalgesia in the inflamed paw with a dose of 3 mg/kg and showed an oral ED₅₀ value of 1.4 mg/kg.

Animal Model:	Male Sprague-Dawley rats^[1]
Dosage:	0.3 and 3 mg/kg
Administration:	Oral gavage; 0.3 and 3 mg/kg once
Result:	Produced robust antidepressant-like activity in the rat forced swim assay with a dose of 3 mg/kg.

Clinical Trial

Molecular Weight

428.95

Formula

C₂₄H₂₉ClN₂O₃

CAS No.

850173-95-4

Appearance

Solid

Color

White to off-white

SMILES

OC1=C2C(OC3(C=C2C4=CC=C(C=C4)C(N(CC)CC)=O)CCNCC3)=CC=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (233.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (11.66 mM; ultrasonic and warming and heat to 60°C)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3313 mL	11.6564 mL	23.3127 mL
5 mM	0.4663 mL	2.3313 mL	4.6625 mL
10 mM	0.2331 mL	1.1656 mL	2.3313 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (6.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (6.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (282 KB) SDS (252 KB)

COA (253 KB) LCMS (121 KB)

Handling Instructions (2659 KB)

References

[1]. Le Bourdonnec B, et al. Potent, orally bioavailable delta opioid receptor agonists for the treatment of pain: discovery of N,N-diethyl-4-(5-hydroxyspiro[chromene-2,4'-piperidine]-4-yl)benzamide (ADL5859). J Med Chem. 2008 Oct 9;51(19):5893-6. [Content Brief]

[2]. Le Bourdonnec B, et al. Spirocyclic delta opioid receptor agonists for the treatment of pain: discovery of N,N-diethyl-3-hydroxy-4-(spiro[chromene-2,4'-piperidine]-4-yl) benzamide (ADL5747).J Med Chem. 2009 Sep 24;52(18):5685-702. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.3313 mL	11.6564 mL	23.3127 mL	58.2818 mL
	5 mM	0.4663 mL	2.3313 mL	4.6625 mL	11.6564 mL
	10 mM	0.2331 mL	1.1656 mL	2.3313 mL	5.8282 mL
DMSO	15 mM	0.1554 mL	0.7771 mL	1.5542 mL	3.8855 mL
	20 mM	0.1166 mL	0.5828 mL	1.1656 mL	2.9141 mL
	25 mM	0.0933 mL	0.4663 mL	0.9325 mL	2.3313 mL
	30 mM	0.0777 mL	0.3885 mL	0.7771 mL	1.9427 mL
	40 mM	0.0583 mL	0.2914 mL	0.5828 mL	1.4570 mL
	50 mM	0.0466 mL	0.2331 mL	0.4663 mL	1.1656 mL
	60 mM	0.0389 mL	0.1943 mL	0.3885 mL	0.9714 mL
	80 mM	0.0291 mL	0.1457 mL	0.2914 mL	0.7285 mL
	100 mM	0.0233 mL	0.1166 mL	0.2331 mL	0.5828 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.