EF24

Name: EF24
Brand: MedChemExpress
SKU: HY-119272
Rating: 4.5 (1 reviews)

Cat. No.: HY-119272 Purity: 99.34%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

EF24 is a curcumin analogue with greater anti-tumor efficacy and oral bioavailability via deactivation of the MAPK/ERK signaling pathway in oral squamous cell carcinoma (OSCC). EF24 treatment increases the levels of activated caspase 3 and 9, and decreases the phosphorylated forms of MEK1 and ERK.

For research use only. We do not sell to patients.

EF24 Chemical Structure

CAS No. : 342808-40-6

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE EF24

•Phytomedicine. 2024 Jul 27:133:155882. [Abstract]

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All ERK Isoform Specific Products:

View All Isoforms

ERK ERK1 ERK2 ERK5 ERK8

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
518A2	IC₅₀	1.8 μM Compound: 2; EF24	Cytotoxicity against human 518A2 cells after 72 hrs by MTT assay Cytotoxicity against human 518A2 cells after 72 hrs by MTT assay	[PMID: 28774574]
A2780	IC₅₀	0.5 μM Compound: EF24	Cell viability of human cisplatin-sensitive human A2780 cells after 24 hrs by MTT assay Cell viability of human cisplatin-sensitive human A2780 cells after 24 hrs by MTT assay	[PMID: 17684018]
A2780	IC₅₀	0.65 μM Compound: EF24	Cell viability of cisplatin-resistant human A2780 cells after 24 hrs by MTT assay Cell viability of cisplatin-resistant human A2780 cells after 24 hrs by MTT assay	[PMID: 17684018]
A549	IC₅₀	2.4 μM Compound: EF24	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
A549	IC₅₀	2.8 μM Compound: A1; EF24	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 29351887]
A549	IC₅₀	7.1 μM Compound: EF24	Cytotoxicity against human A549 cells measured after 72 hrs by MTT assay Cytotoxicity against human A549 cells measured after 72 hrs by MTT assay	[PMID: 27886548]
CHO	IC₅₀	4.6 μM Compound: EF24	Cell viability of CHO cells after 24 hrs by MTT assay Cell viability of CHO cells after 24 hrs by MTT assay	[PMID: 17684018]
DLD-1	IC₅₀	1.3 μM Compound: 2; EF24	Cytotoxicity against human DLD1 cells after 72 hrs by MTT assay Cytotoxicity against human DLD1 cells after 72 hrs by MTT assay	[PMID: 28774574]
HCT-116	IC₅₀	1.5 μM Compound: 2; EF24	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 28774574]
HEK293	CC₅₀	3.58 μM Compound: 33	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability at 20 uM measured after 48 hrs by resazurin reagent based multilabel reader analysis Cytotoxicity against human HEK293 cells assessed as reduction in cell viability at 20 uM measured after 48 hrs by resazurin reagent based multilabel reader analysis	[PMID: 36608774]
HeLa 229	IC₅₀	1.9 μM Compound: A1; EF24	Antiproliferative activity against human HeLa 229 cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa 229 cells after 72 hrs by MTT assay	[PMID: 29351887]
HepG2	IC₅₀	3.33 μM Compound: EF24	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30771605]
HT-29	IC₅₀	1.6 μM Compound: 2; EF24	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 28774574]
L02	IC₅₀	10.2 μM Compound: EF24	Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30771605]
L02	IC₅₀	5.1 μM Compound: EF24	Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
L02	IC₅₀	5.8 μM Compound: EF24	Antiproliferative activity against human HL7702 cells by MTT assay Antiproliferative activity against human HL7702 cells by MTT assay	[PMID: 31336310]
Lewis lung carcinoma cell line	IC₅₀	8.4 μM Compound: EF24	Cytotoxicity against mouse LLC cells measured after 72 hrs by MTT assay Cytotoxicity against mouse LLC cells measured after 72 hrs by MTT assay	[PMID: 27886548]
MGC-803	IC₅₀	2 μM Compound: A1; EF24	Antiproliferative activity against human MGC803 cells after 72 hrs by MTT assay Antiproliferative activity against human MGC803 cells after 72 hrs by MTT assay	[PMID: 29351887]
NB-4	IC₅₀	0.29 μM Compound: 42	Cytotoxicity against human NB4 cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against human NB4 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 24960549]
NCI-H1650	IC₅₀	1.7 μM Compound: EF24	Cytotoxicity against human NCI-H1650 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human NCI-H1650 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
NCI-H1650	IC₅₀	14.6 μM Compound: EF24	Cytotoxicity against human NCI-H1650 cells measured after 72 hrs by MTT assay Cytotoxicity against human NCI-H1650 cells measured after 72 hrs by MTT assay	[PMID: 27886548]
NCI-H1975	IC₅₀	1.3 μM Compound: EF24	Cytotoxicity against human NCI-H1975 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human NCI-H1975 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
NCI-H441	IC₅₀	44 μM Compound: 1	Antiproliferative activity against human H441 cells assessed as decrease of hexosaminidase activity after 24 hrs Antiproliferative activity against human H441 cells assessed as decrease of hexosaminidase activity after 24 hrs	[PMID: 20638855]
NCI-H460	IC₅₀	1.2 μM Compound: EF24	Cytotoxicity against human H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
NCI-H460	IC₅₀	6 μM Compound: EF24	Antiproliferative activity against human H460 cells by MTT assay Antiproliferative activity against human H460 cells by MTT assay	[PMID: 31336310]
PANC-1	IC₅₀	1.5 μM Compound: 2; EF24	Cytotoxicity against human PANC1 cells after 72 hrs by MTT assay Cytotoxicity against human PANC1 cells after 72 hrs by MTT assay	[PMID: 28774574]
QGY-7703	IC₅₀	4.97 μM Compound: EF24	Cytotoxicity against human QGY7703 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human QGY7703 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30771605]
SGC-7901	IC₅₀	1 μM Compound: A1; EF24	Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay	[PMID: 29351887]
SGC-7901	IC₅₀	6.5 μM Compound: EF24	Antiproliferative activity against human SGC7901 cells by MTT assay Antiproliferative activity against human SGC7901 cells by MTT assay	[PMID: 31336310]
SMMC-7721	IC₅₀	8.8 μM Compound: EF24	Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30771605]
U-251	IC₅₀	4.5 μM Compound: EF24	Antiproliferative activity against human U251 cells by MTT assay Antiproliferative activity against human U251 cells by MTT assay	[PMID: 31336310]
U-87MG ATCC	IC₅₀	1.4 μM Compound: 2; EF24	Cytotoxicity against human U87 cells after 72 hrs by MTT assay Cytotoxicity against human U87 cells after 72 hrs by MTT assay	[PMID: 28774574]

Molecular Weight

311.33

Formula

C₁₉H₁₅F₂NO

CAS No.

342808-40-6

Appearance

Solid

Color

White to yellow

SMILES

O=C1/C(CNC/C1=C\C2=C(C=CC=C2)F)=C/C3=C(C=CC=C3)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (160.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2120 mL	16.0601 mL	32.1203 mL
5 mM	0.6424 mL	3.2120 mL	6.4241 mL
10 mM	0.3212 mL	1.6060 mL	3.2120 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.03 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.03 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.34%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB) HNMR (256 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Lelli D, et al. Curcumin and treatment of melanoma: The potential role of microRNAs. Biomed Pharmacother. 2017 Apr;88:832-834. [Content Brief]

[2]. Mosley CA, et al. Highly active anticancer curcumin analogues. Adv Exp Med Biol. 2007;595:77-103. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2120 mL	16.0601 mL	32.1203 mL	80.3006 mL
	5 mM	0.6424 mL	3.2120 mL	6.4241 mL	16.0601 mL
	10 mM	0.3212 mL	1.6060 mL	3.2120 mL	8.0301 mL
	15 mM	0.2141 mL	1.0707 mL	2.1414 mL	5.3534 mL
	20 mM	0.1606 mL	0.8030 mL	1.6060 mL	4.0150 mL
	25 mM	0.1285 mL	0.6424 mL	1.2848 mL	3.2120 mL
	30 mM	0.1071 mL	0.5353 mL	1.0707 mL	2.6767 mL
	40 mM	0.0803 mL	0.4015 mL	0.8030 mL	2.0075 mL
	50 mM	0.0642 mL	0.3212 mL	0.6424 mL	1.6060 mL
	60 mM	0.0535 mL	0.2677 mL	0.5353 mL	1.3383 mL
	80 mM	0.0402 mL	0.2008 mL	0.4015 mL	1.0038 mL
	100 mM	0.0321 mL	0.1606 mL	0.3212 mL	0.8030 mL

EF24 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EF24342808-40-6EF 24EF-24ERKCaspaseExtracellular signal regulated kinasesInhibitorinhibitorinhibit

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EF24

Customer Review

EF24 Related Antibodies

1 Publications Citing Use of MCE EF24

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All ERK Isoform Specific Products:

View All Caspase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

EF24 Related Classifications

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