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Chromogenic Substrate

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Amyloid-β (1) Angiotensin-converting Enzyme (ACE) (2) Antibiotic (2) Bacterial (1) Beta-lactamase (2) Biochemical Assay Reagents (31) Caspase (5) Cathepsin (1) Cholinesterase (ChE) (1) Cytochrome P450 (3) Dengue virus (1) Dipeptidyl Peptidase (1) DNA Stain (1) Endogenous Metabolite (3) Factor Xa (2) Flavivirus (1) Fluorescent Dye (66) Glucosidase (3) HCV Protease (1) HIV Protease (1) MMP (2) NO Synthase (1) Phosphatase (4) Phospholipase (1) Poly(ADP-ribose) Glycohydrolase (PARG) (1) Reactive Oxygen Species (5) Ser/Thr Protease (5) Thrombin (1)
By Research Areas:	Cancer (8) Cardiovascular Disease (4) Infection (9) Inflammation/Immunology (1) Metabolic Disease (8) Neurological Disease (1)

By Targets:

Amyloid-β (1)

Angiotensin-converting Enzyme (ACE) (2)

Antibiotic (2)

Bacterial (1)

Beta-lactamase (2)

Biochemical Assay Reagents (31)

Caspase (5)

Cathepsin (1)

Cholinesterase (ChE) (1)

Cytochrome P450 (3)

Dengue virus (1)

Dipeptidyl Peptidase (1)

DNA Stain (1)

Endogenous Metabolite (3)

Factor Xa (2)

Flavivirus (1)

Fluorescent Dye (66)

Glucosidase (3)

HCV Protease (1)

HIV Protease (1)

MMP (2)

NO Synthase (1)

Phosphatase (4)

Phospholipase (1)

Poly(ADP-ribose) Glycohydrolase (PARG) (1)

Reactive Oxygen Species (5)

Ser/Thr Protease (5)

Thrombin (1)

By Research Areas:

Cancer (8)

Cardiovascular Disease (4)

Infection (9)

Inflammation/Immunology (1)

Metabolic Disease (8)

Neurological Disease (1)

140

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides

Natural
Products

Targets Recommended: MARCKS

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-P4202

Suc-Ala-Glu-Pro-Phe-pNA Suc-AEPF-pNA	Biochemical Assay Reagents	Others
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) is a chromogenic substrate for the peptidylprolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA can be used to evaluate the inhibitory effect of the target compound on Pin1, and catalytic activity of Pin1, etc .

HY-W116594

X-GalNAc	Fluorescent Dye	Infection
X-GalNAc is a chromogenic substrate for for N-acetyl-β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates .

HY-114522

X-alpha-Gal	Biochemical Assay Reagents	Others
X-alpha-Gal is a chromogenic substrate that is used to screen colonies with high activity of β-galactopyranoside .

HY-W039892

4-Nitrophenyl β-D-glucuronide pNPG_1	Others	Others
4-Nitrophenyl β-D-glucuronide (pNPG_1) is aderivative of 4-nitrophenol 4-Nitrophenyl β-D-glucuronide is a chromogenic compound and is the **chromogenic substrate** for β-glucuronidase .

HY-P3949

Fluorescent Substrate for Glu-Specific Proteases	Fluorescent Dye	Others
Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate .

HY-W357151

6-Chloro-3-indoxyl-α-D-mannopyranoside	Fluorescent Dye	Others
6-Chloro-3-indoxyl-α-D-mannopyranoside is a chromogenic substrate for α-mannosidase resulting in a salmon colored precipitate upon cleavage.

HY-W320032

5-Bromo-1H-indol-3-yl octanoate	Fluorescent Dye	Others
5-Bromo-1H-indol-3-yl octanoate is a chromogenic substrate for esterase with C8 activity. 5-Bromo-1H-indol-3-yl octanoate yields a blue precipitate when cleaved.

HY-W345102

4-Methylumbelliferyl β-cellotrioside	Fluorescent Dye	Metabolic Disease
4-Methylumbelliferyl β-cellotrioside, a chromogenic substrate for β-glycosidases, is a cellulose fluorescent derivative .

HY-P4202A

Suc-Ala-Glu-Pro-Phe-pNA TFA Suc-AEPF-pNA TFA	Biochemical Assay Reagents	Others
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA ) TFA is a chromogenic substrate for the peptidylprolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA TFA can be used to evaluate the inhibitory effect of the target compound on Pin1, and catalytic activity of Pin1, etc .

HY-W141825

N-Acetyl-DL-phenylalanine β-naphthyl ester	Fluorescent Dye	Infection Metabolic Disease
N-Acetyl-DL-phenylalanine β-naphthyl ester is an aromatic amino acid ester, which functions as a chromogenic substrate for chymotrypsin and microbial serine proteases such as subtilisin .

HY-W015996

4-Nitrophenyl-N-acetyl-α-D-galactosaminide pNP-GlcNAc	Fluorescent Dye	Others
4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GlcNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection .

HY-P10083

Ac-VQVD-pNA	Fluorescent Dye	Others
Ac-VQVD-pNA is a chromogenic substrate of caspase 3 .

HY-P10094

β-Ala-Gly-Arg-pNA	Fluorescent Dye	Others
β-Ala-Gly-Arg-pNA is a chromogenic substrate of thrombin with pNA a strong absorbance at 405 nm .

HY-P4458

Boc-QAR-pNA	Ser/Thr Protease	Cancer
Boc-QAR-pNA is a chromogenic substrate of trypsin. Boc-QAR-pNA can be used to test trypsin activity .

HY-P4518

Z-Val-Gly-Arg-pNA	Ser/Thr Protease	Others
Z-Val-Gly-Arg-pNA is a chromogenic substrate for urokinase. Z-Val-Gly-Arg-pNA can be used for determination of urokinase activity .

HY-P3934

HIV Protease Substrate I	HIV Protease	Infection
HIV Protease Substrate I is a chromogenic substrate of HIV-1 protease. HIV Protease Substrate I has the cleavage site of HIV protease .

HY-P4475

Sar-Pro-Arg-pNA	Thrombin	Cardiovascular Disease
Sar-Pro-Arg-pNA is a chromogenic substrate of α-thrombin. Sar-Pro-Arg-pNA can be used to test α-thrombin activity .

HY-P4460

AAA-pNA	Ser/Thr Protease	Cancer
AAA-pNA is a chromogenic substrate of Tripeptidyl-peptidase II. AAA-pNA can be used to test Tripeptidyl-peptidase II activity .

HY-P4482

Suc-AAPE-pNA	Bacterial	Infection
Suc-AAPE-pNA is a chromogenic substrate of glutamyl endopeptidase. Suc-AAPE-pNA can be used to test glutamyl endopeptidase activity .

HY-P4464

Ala-Phe-Pro-pNA	Ser/Thr Protease	Cancer
Ala-Phe-Pro-pNA is a chromogenic substrate of tripeptidyl peptidase. Ala-Phe-Pro-pNA can be used to test tripeptidyl peptidase activity .

HY-164207

VLPA-GlcNAc	Biochemical Assay Reagents	Infection
VLPA-GlcNAc is a chromogenic glucosaminide substrate. VLPA-GlcNAc can be used to prepare agar media for the identification of Candida spp .

HY-P4470

Phe-Pro-Ala-pNA	Ser/Thr Protease	Cancer
Phe-Pro-Ala-pNA is a chromogenic substrate of tripeptidyl peptidase. Phe-Pro-Ala-pNA can be used to test tripeptidyl peptidase activity .

HY-114958

RH 421	Fluorescent Dye	Others
RH 421 is a voltage-sensitive styryl dye that can penetrate through the lipid bilayers. RH 421 is a chromogenic substrate for β-galactosidase .

HY-P0021A

D-Lys(Z)-Pro-Arg-pNA diacetate Spectrozyme PCa; Chromozym Pca diacetate	Fluorescent Dye	Others
D-Lys(Z)-Pro-Arg-pNA diacetate (Spectrozyme PCa) is a chromogenic substrate.

HY-W009454

Potassium p-nitrophenyl sulfate 4-Nitopheyl Sulfate potassium	Fluorescent Dye	Others
p-Nitrophenyl Sulfate Potassium is a chromogenic substrate for arylsulfatase. p-Nitrophenyl Sulfate Potassium is released by arylsulfonase cleavage, and the activity of arylsulfonase can be quantitatively determined by colorimetric detection at 400 nm .

HY-15928

PNPP disodium 3 Publications Verification Para-nitrophenyl phosphate disodium	Fluorescent Dye	Others
PNPP (Para-nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

HY-P2685

MeOSuc-Ala-Ala-Pro-Val-pNA	Fluorescent Dye	Others
MeOSuc-Ala-Ala-Pro-Val-pNA is a sensitive chromogenic substrate for human leukocyte and porcine pancreatic elastase .

HY-137816

6-Chloro-3-indolyl-β-D-glucuronide cyclohexylammonium	Others	Others
6-Chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt is a chromogenic substrate for β-glucuronidase. 6-Chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt produces a salmon colored precipitate upon cleavage .

HY-15909

BCIP BCIP p-toluidine salt; X-phosphate p-toluidine salt	Fluorescent Dye	Others
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.

HY-52112

BCDA 5-bromo-4-chloroindoxyl acetate	Fluorescent Dye	Others
BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase .

HY-W330621

2-Chloro-4-nitrophenyl-α-D-glucopyranoside CNP-α-D-Glucopyaoside	Fluorescent Dye	Others
2-Chloro-4-nitrophenyl-α-D-glucopyranoside (CNP-α-D-Glucopyaoside) as a chromogenic substrate for the enzymatic activity assay of the enzyme that releases CNP from conjugated carbohydrates .

HY-137276

5-Bromo-3-indolyl β-D-galactopyranoside Bluo-Gal	Fluorescent Dye	Others
5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate to detect bacterial β-D-galactosidase activity. 5-Bromo-3-indolyl β-D-galactopyranoside is used both to recognize labelled myofibers, and beta-gal positive organelles inside single myofibers .

HY-123275A

H-D-Phe-Pip-Arg-pNA hydrochloride S-2238 hydrochloride	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) hydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA hydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA hydrochloride is sensitive, accurate, and easy to perform .

HY-123275

H-D-Phe-Pip-Arg-pNA S-2238	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238), a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA is sensitive, accurate, and easy to perform .

HY-123275C

H-D-Phe-Pip-Arg-pNA dihydrochloride S-2238 dihydrochloride	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) dihydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA dihydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA dihydrochloride is sensitive, accurate, and easy to perform .

HY-123275B

H-D-Phe-Pip-Arg-pNA acetate S-2238 acetate	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) acetate, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA acetate is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA acetate is sensitive, accurate, and easy to perform .

HY-D1676

Thymolphthalein monophosphate disodium hydrate	Phosphatase	Others
Thymolphthalein monophosphate disodium hydrate is a chromogenic substrate for the determination of acid phosphatase and alkaline phosphatase. Thymolphthalein is released during the reaction, increases the pH of the medium for easy detection, produces color and stops hydrolysis. Thymolphthalein monophosphate disodium hydrate can be used for the specific detection of prostatic phosphatase in serum .

HY-P4039

Ac-EEVVAC-pNA	HCV Protease	Infection
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

HY-W411361

Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone PCNPH	Fluorescent Dye	Cancer
Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone (PCNPH) is a chromogenic substrate to peroxidase enzymes. Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone can form a purple indamine dye with peroxidase enzymes and peroxides .

HY-124324

4-Nitrophenyl β-D-cellotrioside p-Nitopheyl β-D-cellotioside	Fluorescent Dye	Others
4-Nitrophenyl β-D-cellotrioside (p-Nitopheyl β-D-cellotioside) is a chromogenic substrate for endoglucanases and cellulose biohydrolases. As a fluorescent dye, nitrophenyl β-D-Cellotrioside can be hydrolyzed by enzymes to release 4-nitrophenol, producing a yellow color. The activity of the enzyme can be quantitatively analyzed by monitoring the change in absorbance at 405 nm .

HY-15934

X-GAL 1 Publications Verification BCIG	Fluorescent Dye Glucosidase	Others
X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity .

HY-P0022

pGlu-Pro-Arg-MNA	Biochemical Assay Reagents	Others
pGlu-Pro-Arg-MNA is a chromogenic substrate.

HY-P0022A

pGlu-Pro-Arg-MNA monoacetate	Biochemical Assay Reagents	Others
pGlu-Pro-Arg-MNA monoacetate is a chromogenic substrate.

HY-P3936

Endotoxin Substrate	Fluorescent Dye	Others
Endotoxin Substrate (Boc-LGR-pNA) is a chromogenic substrate can be used in quantitative assays of endotoxin .

HY-P10448

Boc-Leu-Gly-Arg-pNA acetate Boc-LGR-pNA acetate	Fluorescent Dye	Others
Boc-Leu-Gly-Arg-pNA is a chromogenic synthetic substrate .

HY-137228

4-Nitrophenylmaltohexaoside	Biochemical Assay Reagents	Others
4-Nitrophenylmaltohexaoside is a chromogenic substrate targeting α-amylase.

HY-W328317

Fast garnet GBC sulfate salt, 90% Azoic diazo No. 4, 90%	Biochemical Assay Reagents	Others
Fast garnet GBC sulfate salt, 90% is a chromogenic substrate for alkaline phosphatase.

HY-138152

Z-D-Arg-Gly-Arg-pNA dihydrochloride Benzyl-D-Arg-Gly-Arg-pNA dihydrochloride	Fluorescent Dye	Others
Z-D-Arg-Gly-Arg-pNA dihydrochloride is a hydrolytic chromogenic plasmin substrate .

HY-D1213

4-Nitrocatechol sulfate dipotassium salt Dipotassium nitrocatechol sulfate	Biochemical Assay Reagents	Others
4-Nitrocatechol sulfate dipotassium salt (Dipotassium nitrocatechol sulfate) is a chromogenic substrate for sulphatase .

HY-W341421

3-Indoxyl butyrate	Biochemical Assay Reagents	Others
3-Indoxyl butyrate is a chromogenic substrate for CES (carboxylesterase), producing a blue precipitate upon cleavage.

Cat. No.	Product Name	Type

HY-P4202

Suc-Ala-Glu-Pro-Phe-pNA Suc-AEPF-pNA	Chromogenic Substrates
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) is a chromogenic substrate for the peptidylprolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA can be used to evaluate the inhibitory effect of the target compound on Pin1, and catalytic activity of Pin1, etc .

HY-W116594

X-GalNAc	Chromogenic Substrates
X-GalNAc is a chromogenic substrate for for N-acetyl-β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates .

HY-P0021A

D-Lys(Z)-Pro-Arg-pNA diacetate Spectrozyme PCa; Chromozym Pca diacetate	Chromogenic Substrates
D-Lys(Z)-Pro-Arg-pNA diacetate (Spectrozyme PCa) is a chromogenic substrate.

HY-W357151

6-Chloro-3-indoxyl-α-D-mannopyranoside	Chromogenic Substrates
6-Chloro-3-indoxyl-α-D-mannopyranoside is a chromogenic substrate for α-mannosidase resulting in a salmon colored precipitate upon cleavage.

HY-W320032

5-Bromo-1H-indol-3-yl octanoate	Chromogenic Substrates
5-Bromo-1H-indol-3-yl octanoate is a chromogenic substrate for esterase with C8 activity. 5-Bromo-1H-indol-3-yl octanoate yields a blue precipitate when cleaved.

HY-W345102

4-Methylumbelliferyl β-cellotrioside	Chromogenic Substrates
4-Methylumbelliferyl β-cellotrioside, a chromogenic substrate for β-glycosidases, is a cellulose fluorescent derivative .

HY-P4202A

Suc-Ala-Glu-Pro-Phe-pNA TFA Suc-AEPF-pNA TFA	Chromogenic Substrates
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA ) TFA is a chromogenic substrate for the peptidylprolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA TFA can be used to evaluate the inhibitory effect of the target compound on Pin1, and catalytic activity of Pin1, etc .

HY-W141825

N-Acetyl-DL-phenylalanine β-naphthyl ester	Chromogenic Substrates
N-Acetyl-DL-phenylalanine β-naphthyl ester is an aromatic amino acid ester, which functions as a chromogenic substrate for chymotrypsin and microbial serine proteases such as subtilisin .

HY-W015996

4-Nitrophenyl-N-acetyl-α-D-galactosaminide pNP-GlcNAc	Chromogenic Substrates
4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GlcNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection .

HY-114958

RH 421	Fluorescent Dyes/Probes
RH 421 is a voltage-sensitive styryl dye that can penetrate through the lipid bilayers. RH 421 is a chromogenic substrate for β-galactosidase .

HY-W009454

Potassium p-nitrophenyl sulfate 4-Nitopheyl Sulfate potassium	Dyes
p-Nitrophenyl Sulfate Potassium is a chromogenic substrate for arylsulfatase. p-Nitrophenyl Sulfate Potassium is released by arylsulfonase cleavage, and the activity of arylsulfonase can be quantitatively determined by colorimetric detection at 400 nm .

HY-15928

PNPP disodium 3 Publications Verification Para-nitrophenyl phosphate disodium	Chromogenic Substrates
PNPP (Para-nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

HY-P2685

MeOSuc-Ala-Ala-Pro-Val-pNA	Chromogenic Substrates
MeOSuc-Ala-Ala-Pro-Val-pNA is a sensitive chromogenic substrate for human leukocyte and porcine pancreatic elastase .

HY-15909

BCIP BCIP p-toluidine salt; X-phosphate p-toluidine salt	Chromogenic Substrates
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.

HY-52112

BCDA 5-bromo-4-chloroindoxyl acetate	Chromogenic Substrates
BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase .

HY-W330621

2-Chloro-4-nitrophenyl-α-D-glucopyranoside CNP-α-D-Glucopyaoside	Fluorescent Dyes/Probes
2-Chloro-4-nitrophenyl-α-D-glucopyranoside (CNP-α-D-Glucopyaoside) as a chromogenic substrate for the enzymatic activity assay of the enzyme that releases CNP from conjugated carbohydrates .

HY-D1676

Thymolphthalein monophosphate disodium hydrate

Fluorescent Dyes/Probes

Thymolphthalein monophosphate disodium hydrate is a chromogenic substrate for the determination of acid phosphatase and alkaline phosphatase. Thymolphthalein is released during the reaction, increases the pH of the medium for easy detection, produces color and stops hydrolysis. Thymolphthalein monophosphate disodium hydrate can be used for the specific detection of prostatic phosphatase in serum .

HY-P4039

Ac-EEVVAC-pNA	Chromogenic Substrates
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

HY-W411361

Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone PCNPH	Chromogenic Substrates
Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone (PCNPH) is a chromogenic substrate to peroxidase enzymes. Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone can form a purple indamine dye with peroxidase enzymes and peroxides .

HY-124324

4-Nitrophenyl β-D-cellotrioside p-Nitopheyl β-D-cellotioside	Fluorescent Dyes/Probes
4-Nitrophenyl β-D-cellotrioside (p-Nitopheyl β-D-cellotioside) is a chromogenic substrate for endoglucanases and cellulose biohydrolases. As a fluorescent dye, nitrophenyl β-D-Cellotrioside can be hydrolyzed by enzymes to release 4-nitrophenol, producing a yellow color. The activity of the enzyme can be quantitatively analyzed by monitoring the change in absorbance at 405 nm .

HY-15934

X-GAL 1 Publications Verification BCIG	Chromogenic Substrates
X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity .

HY-D1213

4-Nitrocatechol sulfate dipotassium salt Dipotassium nitrocatechol sulfate	Dyes
4-Nitrocatechol sulfate dipotassium salt (Dipotassium nitrocatechol sulfate) is a chromogenic substrate for sulphatase .

HY-32264

X-Neu5Ac sodium X-NeuNAc	Fluorescent Dyes/Probes
X-Neu5Ac (sodium) is a substrate for chromogenic assay of neuraminidase activity in bacterial expression systems; with a K_m of 0.89 mM for neuraminidase.

HY-W154295

Purple-β-D-Gal	Fluorescent Dyes/Probes
Purple-β-D-Gal is a chromogenic β-galactosidase substrate. Intracellular enzymatic hydrolysis of Purple-β-D-Gal generates free indoxyl molecules, which undergo in situ oxidation and subsequent dimerization to produce chromogenic, water-insoluble, indigo precipitates. Purple-β-D-Gal can be used for the detection of β-galactosidase activity .

HY-100045

p-Nitrophenyl phosphorylcholine 4-Nitrophenylphosphorylcholine; 4-Nitrophenylphosphorylcholine; O-(4-Nitrophenylphosphoryl)choline	Fluorescent Dyes/Probes
p-Nitrophenyl phosphorylcholine (4-Nitrophenylphosphorylcholine) is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.

HY-W713925

Diheptanoyl Thio-PC	Dyes
Diheptanoyl Thio-PC is a substrate for all phospholipase A2s (PLA2s) with the exception of cPLA2 and PAF-acetyl hydrolase (PAF-AH).1 Interaction of this compound with a PLA2 results in cleavage of the sn-2 fatty acid generating a free thiol on the lysophospholipid. This free thiol can be detected using chromogenic substrates such as DTNB (Ellman’s reagent) and DTP.

HY-15902

ABTS diammonium salt 3 Publications Verification AzBTS-(NH4)2	Chromogenic Substrates
ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.

HY-15914

DHBS DCHBS	Chromogenic Substrates
DHBS (3, 5-dichloro-2-hydroxybenzenesulfonate) is used in conjunction with 4-aminoantipyrine (4-AAP) (HY-W100990) and hydrogen peroxide (H₂O₂) for chromogenic quantitation of peroxidase in coupled enzymatic reactions. Component of Trinder reagent for use with peroxidase to measure generation of hydrogen peroxide in automated systems .

HY-15924

Thiazolyl Blue Maximum Cited Publications 118 Publications Verification MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide	Chromogenic Substrates
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .

HY-15925

Nitro blue tetrazolium chloride 5+ Cited Publications NBT	Chromogenic Substrates
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures .

HY-15930

TMB

1 Publications Verification

BM blue; Sure Blue TMB

Chromogenic Substrates

TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922 .

HY-15930A

TMB dihydrochloride

1 Publications Verification

BM blue dihydrochloride; Sure Blue TMB dihydrochloride

Chromogenic Substrates

TMB dihydrochloride is the dihydrochloride form of TMB (HY-15930). TMB dihydrochloride is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB dihydrochloride can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB dihydrochloride can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922 .

HY-15931

TMB-PS TMBZ-PS	Chromogenic Substrates
TMB-PS(102062-36-2) is N-(3-sulfopropyl)-3,3',5,5'-tetramenthylbenzidine sodium salt; White - pale yellow crystalline powder, soluble in water, can be used under neutral, acidic and alkaline conditions.

HY-16710

GPDA GPN; Glycylproline p-nitroanilide tosylate	Chromogenic Substrates
GPDA(Glycylproline p-nitroanilide tosylate) is the substarate of X-Prolyl dipeptidyl-aminopeptidase in the enzyme assay.

HY-P0019

Z-Gly-Gly-Arg-AMC 5+ Cited Publications	Chromogenic Substrates
Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).

HY-P0021

D-Lys(Z)-Pro-Arg-pNA Chromozym Pca	Chromogenic Substrates
D-Lys(Z)-Pro-Arg-pNA (Chromozym Pca) is a luminescent substrate of activated protein C (APC).

HY-P0019A

Z-Gly-Gly-Arg-AMC acetate 5+ Cited Publications	Chromogenic Substrates
Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).

HY-12591

D-Luciferin sodium

40+ Cited Publications

D-(-)-Luciferin sodium; Firefly luciferin sodium; Beetle Luciferin sodium

Chromogenic Substrates

D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We of er the firefly luciferase (HY-P1004), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .

HY-12591A

D-Luciferin

40+ Cited Publications

D-(-)-Luciferin; Firefly luciferin; Beetle Luciferin

Chromogenic Substrates

HY-D0055

3-Cyano-7-ethoxycoumarin	Chromogenic Substrates
3-Cyano-7-ethoxycoumarin is a fluorogenic cytochrome P-450 substrate that generates blue fluorescent product upon enzyme cleavage Target: Cytochrome P450 3-Cyano-7-ethoxycoumarin is a fluorescent probe useful in microsomal dealkylase studies.

HY-D0829

3,6-Dichlorotrimellitic anhydride	Chromogenic Substrates
3,6-Dichlorotrimellitic anhydride is the key precursor that is used for preparing a variety of dichlorinated fluoresceins and rhodamines such as TET and HEX.

HY-N0116

Hematoxylin 5+ Cited Publications Natural Black 1; Haematoxylin	Chromogenic Substrates
Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from Caesalpinia sappan Linn.. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC₅₀ of 1.6 μM.

HY-D0831

WST-8 5+ Cited Publications	Chromogenic Substrates
WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

HY-15930C

TMB monosulfate

1 Publications Verification

BM blue monosulfate; Sure Blue TMB monosulfate

Chromogenic Substrates

TMB monosulfate is the monosulfate form of TMB (HY-15930). TMB monosulfate is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB monosulfate can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB monosulfate can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922 .

HY-12591B

D-Luciferin potassium

40+ Cited Publications

D-(-)-Luciferin potassium; Firefly luciferin potassium; Beetle Luciferin potassium

Chromogenic Substrates

D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We offer the firefly luciferase (HY-P1004), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .

HY-P1003

Ac-DEVD-AMC 4 Publications Verification	Chromogenic Substrates
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .

HY-101895

Fluorescein di(β-D-galactopyranoside) 4 Publications Verification FDG	Chromogenic Substrates
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λ_ex=485 nm, λ_em=535 nm).

HY-D0935A

4-Methylumbelliferyl-β-D-glucuronide hydrate MUG	Chromogenic Substrates
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λ_ex=362 nm, λ_em=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli .

HY-D1026

CUG 3-Carboxyumbelliferyl-β-D-galactopyranoside	Chromogenic Substrates
CUG (3-Carboxyumbelliferyl-β-D-galactopyranoside) is a fluorogenic substrate (λ_ex=386, λ_em=445 nm, ε=32K).

HY-D0994

4-Methylumbelliferyl phosphate 4-MUP; MUP	Chromogenic Substrates
4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant .

Cat. No.	Product Name	Type

HY-137895

L-Arginine p-nitroanilide dihydrochloride, 98% H-Arg-pNA 2HCl, 98%	Enzyme Substrates
L-Arginine p-nitroanilide dihydrochloride, 98% (H-Arg-pNA 2HCl, 98%) is a chromogenic substrate for cathepsin H and aminopeptidases.

HY-137228

4-Nitrophenylmaltohexaoside	Cell Assay Reagents
4-Nitrophenylmaltohexaoside is a chromogenic substrate targeting α-amylase.

HY-W328317

Fast garnet GBC sulfate salt, 90% Azoic diazo No. 4, 90%	Indicators
Fast garnet GBC sulfate salt, 90% is a chromogenic substrate for alkaline phosphatase.

HY-W341421

3-Indoxyl butyrate	Cell Assay Reagents
3-Indoxyl butyrate is a chromogenic substrate for CES (carboxylesterase), producing a blue precipitate upon cleavage.

HY-W638077

6-Chloro-3-indoxyl caprylate	Cell Assay Reagents
6-Chloro-3-indoxyl caprylate is a chromogenic substrate with C8 activity for esterases.

HY-W290273

2-Nitrophenyl-β-D-thiogalactopyranoside 2-Nitrophenyl-beta-D-thiogalactopyranoside	Carbohydrates
2-Nitrophenyl-β-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase in various biological and biochemical assays.

HY-138821

BCIP potassium	Cell Assay Reagents
BCIP (potassium) is a chromogenic substrate commonly used to detect the enzymatic activity of alkaline phosphatase. When dephosphorylated by alkaline phosphatase, BCIP produces a blue precipitate.

HY-W283889

Blue caprate	Cell Assay Reagents
Blue caprate is a chromogenic enzyme substrate typically used to detect lipase activity. It is hydrolyzed by lipase to produce a blue-purple product (biosynth: EB04034).

HY-137785

2-Chloro-4-nitrophenyl-β-D-maltotrioside	Cell Assay Reagents
2-Chloro-4-nitrophenyl-β-D-maltotrioside is a chromogenic substrate used in bioluminescent and fluorescent assays to detect α-amylase.

HY-W101382

5-Bromo-6-chloro-3-indolyl-N-acetyl-β-D-glucosaminide, 98%	Carbohydrates
5-Bromo-6-chloro-3-indolyl-N-acetyl-β-D-glucosaminide, 98% is a chromogenic substrate used to measure β-galactosidase activity in food, enzyme substrates, and culture media.

HY-W288676

6-Fluoro-3-indolyl-β-D-galactopyranoside	Cell Assay Reagents
6-Fluoro-3-indolyl-β-D-galactopyranoside is a chromogenic enzyme substrate commonly used in microbiology to detect the expression of β-galactosidase in bacterial colonies.

HY-W104635

6-Bromo-2-naphthyl-β-D-galactopyranoside 6-Bromo-2-naphthyl-beta-D-galactopyranoside	Carbohydrates
6-Bromo-2-naphthyl-β-D-galactopyranoside is a chromogenic substrate commonly used to measure β-galactosidase enzyme activity in food, enzyme substrates, and culture media. Upon hydrolysis by β-galactosidase, it generates a yellow precipitate indicating the enzyme's presence.

HY-15927

4-Nitrophenyl β-D-glucopyranoside 1 Publications Verification	Enzyme Substrates
4-Nitrophenyl β-D-glucopyranoside is a chromogenic substrate for β-glucosidase. 4-Nitrophenyl β-D-glucopyranoside can be used to measure of β-glucosidase activity .

HY-W293502

2-Chloro-4-nitrophenyl-β-D-galactopyranoside 2-Chloro-4-nitrophenyl-beta-D-galactopyranoside	Carbohydrates
2-Chloro-4-nitrophenyl-β-D-galactopyranoside is a chromogenic substrate used for testing enzyme activity. It is commonly used in molecular biology research to detect and measure the activity of β-galactosidase (biosynth: EC03318).

HY-W587779

o-Nitrophenyl β-D-galactopyranoside-6-phosphate cyclohexylammonium	Enzyme Substrates
o-Nitrophenyl β-D-galactopyranoside-6-phosphate cyclohexylammonium is a chromogenic substrate with activity for β-galactosidase detection. The use of o-Nitrophenyl β-D-galactopyranoside-6-phosphate cyclohexylammonium can help researchers quickly identify and quantify β-galactosidase activity.

HY-W854392

3-Carboxyumbelliferyl-β-D-glucuronide dipotassium

Enzyme Substrates

3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) is a chromogenic substrate with biological activity for enzyme activity detection. 3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) can be used to evaluate biological reactions associated with glycosidases. 3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) shows a wide range of application potentials in compound discovery and biomedical research.

HY-W698964

2′-Hydroxy-5′-nitrohexadecanamide

Indicators

2′-Hydroxy-5′-nitrohexadecanamide is a lysosomal hydrolase inhibitor and is a lipid containing pentadecanoic acid and a terminal nitrophenol in its structure. 2′-Hydroxy-5′-nitrohexadecanamide can cause intracellular lipid accumulation by inhibiting lysosomal hydrolase activity. Additionally, 2′-Hydroxy-5′-nitrohexadecanamide can be used to synthesize chromogenic substrates for measuring sphingolipase activity .

HY-W127380

Arachidonoyl thio-PC

Enzyme Substrates

Arachidonoyl Thio-PC is a substrate of many phospholipase A2 (PLA2), including sPLA2, cPLA2 and iPLA2. Cleavage of sn-2 fatty acids by PLA2 results in the production of free thiols, which react with chromogenic reagents such as DTNB (Ellman's reagent) and DTP, allowing quantification of PLA2 activity. Isozyme-specific cPLA2 activity can be measured by depleting or inhibiting sPLA2 and iPLA2 activity in the assay.

HY-D1208

CDP-Star	Chromogenic Substrates
CDP-Star is a chemiluminescent substrate for alkaline phosphatase. CDP-Star can be used for enzyme-linked immunoassays .

HY-137779

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

Carbohydrates

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, a chromogenic substrate for the detection of β-galactosidase activity. It is commonly used in molecular biology techniques such as gene expression analysis and reporter gene analysis. When β-galactosidase cleaves X-Gluc, a blue precipitate is produced, which can be observed by microscopy or other detection methods. X-Gluc has high sensitivity and specificity for the detection of β-galactosidase activity, making it a widely used tool in molecular biology research.

Cat. No.	Product Name	Category	Target	Chemical Structure

HY-12591A

D-Luciferin 40+ Cited Publications D-(-)-Luciferin; Firefly luciferin; Beetle Luciferin	Natural Products Animals Source classification Other Diseases Disease Research Fields	Fluorescent Dye
D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We of er the firefly luciferase (HY-P1004), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .

HY-N0116

Hematoxylin 5+ Cited Publications Natural Black 1; Haematoxylin	Structural Classification Classification of Application Fields Leguminosae Caesalpinia sappan L. Source classification Other Diseases Phenols Polyphenols Plants Disease Research Fields	Amyloid-β
Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from Caesalpinia sappan Linn.. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC₅₀ of 1.6 μM.

HY-113245

3-Indolyl-β-D-glucuronide	Structural Classification Alkaloids Classification of Application Fields Source classification Other Diseases Endogenous metabolite Disease Research Fields Indole Alkaloids	Endogenous Metabolite
3-Indolyl-β-D-glucuronide, a chromogenic substrate for β-D-glucuronidase, employs in the detection and enumeration of E. coli, yielding a blue precipitate upon cleavage. 3-Indolyl-β-D-glucuronide in? patients' plasma act as a new indicator of renal failure .

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