2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK GPCR/G Protein
  3. EGFR GPR35
  4. Tyrphostin 25

Tyrphostin 25  (Synonyms: AG82; Tyrphostin A 25; Tyrphostin AG 82; RG-50875)

Cat. No.: HY-101958 Purity: 99.00%
SDS COA Handling Instructions Technical Support

Tyrphostin 25 (AG82) is a specific inhibitor of the EGFR tyrosine kinase. Tyrphostin 25 is also a GPR35 agonist with an IC50 of 0.94 µM and an EC50 of 5.3 µM.

For research use only. We do not sell to patients.

Tyrphostin 25 Chemical Structure

Tyrphostin 25 Chemical Structure

CAS No. : 118409-58-8

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5 mg In-stock
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Based on 1 publication(s) in Google Scholar

Tyrphostin 25 Related Antibodies

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Description

Tyrphostin 25 (AG82) is a specific inhibitor of the EGFR tyrosine kinase. Tyrphostin 25 is also a GPR35 agonist with an IC50 of 0.94 µM and an EC50 of 5.3 µM[1][2].

IC50 & Target

EGFR tyrosine kinase[1]
IC50: 0.94 µM (GPR35)[2]
EC50: 5.3 µM (GPR35)[2]
Molecular Weight

202.17

Formula

C10H6N2O3
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

N#C/C(C#N)=C/C1=CC(O)=C(O)C(O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 30 mg/mL (148.39 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.9463 mL 24.7317 mL 49.4633 mL
5 mM 0.9893 mL 4.9463 mL 9.8927 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.00%

SDS (393 KB)

COA (248 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.9463 mL 24.7317 mL 49.4633 mL 123.6583 mL
5 mM 0.9893 mL 4.9463 mL 9.8927 mL 24.7317 mL
10 mM 0.4946 mL 2.4732 mL 4.9463 mL 12.3658 mL
15 mM 0.3298 mL 1.6488 mL 3.2976 mL 8.2439 mL
20 mM 0.2473 mL 1.2366 mL 2.4732 mL 6.1829 mL
25 mM 0.1979 mL 0.9893 mL 1.9785 mL 4.9463 mL
30 mM 0.1649 mL 0.8244 mL 1.6488 mL 4.1219 mL
40 mM 0.1237 mL 0.6183 mL 1.2366 mL 3.0915 mL
50 mM 0.0989 mL 0.4946 mL 0.9893 mL 2.4732 mL
60 mM 0.0824 mL 0.4122 mL 0.8244 mL 2.0610 mL
80 mM 0.0618 mL 0.3091 mL 0.6183 mL 1.5457 mL
100 mM 0.0495 mL 0.2473 mL 0.4946 mL 1.2366 mL
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Tyrphostin 25 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tyrphostin 25118409-58-8AG82 Tyrphostin A 25 Tyrphostin AG 82 RG-50875Tyrphostin25Tyrphostin-25AG 82AG-82Tyrphostin A25Tyrphostin A 25Tyrphostin A-25Tyrphostin AG82Tyrphostin AG 82Tyrphostin AG-82RG50875RG 50875RG-50875EGFRGPR35Epidermal growth factor receptorErbB-1HER1G Protein-Coupled Receptor 35G protein-coupled receptorepidermal growth factor receptorInhibitorinhibitorinhibit

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