2. Apoptosis Autophagy
  3. Ferroptosis Autophagy
  4. WJ460

WJ460 is a potent inhibitor of myoferlin (MYOF) that interacts directly with MYOF. WJ460 inhibits the migration and growth, induces cell cycle arrest, mitochondrial autophagy, lipid peroxidation and ferroptosis in tumor cells. WJ460 has anti-tumor activity.

For research use only. We do not sell to patients.

WJ460 Chemical Structure

WJ460 Chemical Structure

CAS No. : 1415251-36-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 113 In-stock
Solution
10 mM * 1 mL in DMSO USD 113 In-stock
Solid
5 mg USD 103 In-stock
10 mg USD 161 In-stock
25 mg USD 286 In-stock
50 mg USD 430 In-stock
100 mg USD 625 In-stock
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500 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

WJ460 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

WJ460 is a potent inhibitor of myoferlin (MYOF) that interacts directly with MYOF. WJ460 inhibits the migration and growth, induces cell cycle arrest, mitochondrial autophagy, lipid peroxidation and ferroptosis in tumor cells. WJ460 has anti-tumor activity[1][2].

IC50 & Target

MYOF[1]
In Vitro

WJ460 (50 nM; 16-24 h) induces G2/M phase arrest, mitochondrial autophagy, lipid peroxidation and ferroptosis in PDAC cell lines[1].
WJ460 (1-100 nM; 12 h) blocks the invasion of breast cancer cells, significantly inhibits the invasion of MDA-MB-231 and BT549 cells through collagen I, and the IC50 values in MDA-MB-231 of 43.37 nM and 36.40 nM in BT549 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

WJ460 Related Antibodies

Immunofluorescence[1]

Cell Line: PDAC cell lines
Concentration: 50 nM
Incubation Time: 16 and 24 h
Result: Decreased mitochondrial ROS abundance.

Western Blot Analysis[1]

Cell Line: PaTu 8988T and HPNE cells
Concentration: 50 nM
Incubation Time: 8, 10, 12 and 24 h
Result: Reduced the levels of SLC7A11 and GPX-4.
In Vivo

WJ460 (5-10mg/kg; intraperitoneal injection; single dose) has antitumor activity in nude mouse breast cancer metastasis model[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MDA-MB-231-Luciferase cells treated athymic nude mice[2]
Dosage: 5 and 10mg/kg
Administration: Intraperitoneal injection (i.p.); single dose
Result: Significantly inhibited breast cancer pulmonary metastasis in a concentration-dependent manner.
Inhibited the proliferation of MDA-MB-231 cells.
Increased mouse overall survival.
Molecular Weight

460.59

Formula

C27H28N2O3S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NCCCCC1=CC=CC=C1)C2=CC=CC(C(N3C4=CC=CC=C4OC)SCC3=O)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (542.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1711 mL 10.8556 mL 21.7113 mL
5 mM 0.4342 mL 2.1711 mL 4.3423 mL
View the Complete Stock Solution Preparation Table

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1711 mL 10.8556 mL 21.7113 mL 54.2782 mL
5 mM 0.4342 mL 2.1711 mL 4.3423 mL 10.8556 mL
10 mM 0.2171 mL 1.0856 mL 2.1711 mL 5.4278 mL
15 mM 0.1447 mL 0.7237 mL 1.4474 mL 3.6185 mL
20 mM 0.1086 mL 0.5428 mL 1.0856 mL 2.7139 mL
25 mM 0.0868 mL 0.4342 mL 0.8685 mL 2.1711 mL
30 mM 0.0724 mL 0.3619 mL 0.7237 mL 1.8093 mL
40 mM 0.0543 mL 0.2714 mL 0.5428 mL 1.3570 mL
50 mM 0.0434 mL 0.2171 mL 0.4342 mL 1.0856 mL
60 mM 0.0362 mL 0.1809 mL 0.3619 mL 0.9046 mL
80 mM 0.0271 mL 0.1357 mL 0.2714 mL 0.6785 mL
100 mM 0.0217 mL 0.1086 mL 0.2171 mL 0.5428 mL
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WJ460 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WJ4601415251-36-3WJ 460WJ-460FerroptosisAutophagybreast cancer metastasis modelMDA-MB-231BT549PDAC cellsInhibitorinhibitorinhibit

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