2. Metabolic Enzyme/Protease
  3. Phosphodiesterase (PDE)
  4. DNMDP

DNMDP, a phosphodiesterase 3A (PDE3A) inhibitor, is a potent and selective cancer cell cytotoxic agent. DNMDP binding to PDE3A promotes an interaction between PDE3A and Schlafen 12 (SLFN12). DNMDP shows clear cell-selective cytotoxicity.

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DNMDP Chemical Structure

DNMDP Chemical Structure

CAS No. : 328104-79-6

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of DNMDP:

DNMDP Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

DNMDP, a phosphodiesterase 3A (PDE3A) inhibitor, is a potent and selective cancer cell cytotoxic agent. DNMDP binding to PDE3A promotes an interaction between PDE3A and Schlafen 12 (SLFN12). DNMDP shows clear cell-selective cytotoxicity[1].

IC50 & Target

PDE3

 

Cellular Effect
Cell Line Type Value Description References
HeLa EC50
6.9 nM
Compound: DNMDP
Cytotoxicity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by Caspase-Glo reagent based assay
Cytotoxicity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by Caspase-Glo reagent based assay
[PMID: 31749907]
SK-MEL3 EC50
12 nM
Compound: DNMDP
Cytotoxicity against human SK-MEL3 cells assessed as cell growth inhibition upto 10 uM measured after 48 hrs by Caspase-Glo reagent based assay
Cytotoxicity against human SK-MEL3 cells assessed as cell growth inhibition upto 10 uM measured after 48 hrs by Caspase-Glo reagent based assay
[PMID: 31749907]
In Vitro

DNMDP (0-1000 μM; 48 hours) shows clear cell-selective cytotoxicity[1].
HeLa cells undergo apoptosis upon DNMDP treatment, indicates by caspase activity detected by a caspase-sensitive luciferase assay and by poly ADP ribose polymerase (PARP) cleavage. At a concentration of 100 nM, DNMDP specifically inhibits both PDE3A and PDE3B, weakly inhibits PDE10, and had little or no detectable effect on other phosphodiesterases[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DNMDP Related Antibodies

Cell Viability Assay[1]

Cell Line: NCI-H1563, NCI-H2122, HeLa, A549, MCF7, and PC3 cells
Concentration: 0-1000 μM
Incubation Time: 48 hours
Result: Showed an EC50 between 10 and 100 nM for two additional lung adenocarcinoma cell lines, NCI-H1563 and NCI-H2122, and for HeLa cervical carcinoma cells, but an EC50 greater than 1 µM for A549, MCF7, and PC3 cells.
Molecular Weight

304.34

Formula

C15H20N4O3
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

[O-][N+](C1=CC(C(C(C)C2)=NNC2=O)=CC=C1N(CC)CC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (164.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2858 mL 16.4290 mL 32.8580 mL
5 mM 0.6572 mL 3.2858 mL 6.5716 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.95%

SDS (251 KB)

COA (248 KB) HNMR (261 KB) LCMS (101 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2858 mL 16.4290 mL 32.8580 mL 82.1450 mL
5 mM 0.6572 mL 3.2858 mL 6.5716 mL 16.4290 mL
10 mM 0.3286 mL 1.6429 mL 3.2858 mL 8.2145 mL
15 mM 0.2191 mL 1.0953 mL 2.1905 mL 5.4763 mL
20 mM 0.1643 mL 0.8214 mL 1.6429 mL 4.1072 mL
25 mM 0.1314 mL 0.6572 mL 1.3143 mL 3.2858 mL
30 mM 0.1095 mL 0.5476 mL 1.0953 mL 2.7382 mL
40 mM 0.0821 mL 0.4107 mL 0.8214 mL 2.0536 mL
50 mM 0.0657 mL 0.3286 mL 0.6572 mL 1.6429 mL
60 mM 0.0548 mL 0.2738 mL 0.5476 mL 1.3691 mL
80 mM 0.0411 mL 0.2054 mL 0.4107 mL 1.0268 mL
100 mM 0.0329 mL 0.1643 mL 0.3286 mL 0.8214 mL
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DNMDP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DNMDP328104-79-6Phosphodiesterase (PDE)cell-selectivecytotoxicitySchlafen 12PARPcleavageNCI-H1563NCI-H2122HeLacellsInhibitorinhibitorinhibit

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