2. Others Neuronal Signaling
  3. Biochemical Assay Reagents Amyloid-β
  4. 4-Hydroxyindole

4-Hydroxyindole is a type of hydroxyindole in which the 1H-indole at position 4 is substituted by a hydroxyl group. 4-Hydroxyindole serves as an important raw material or intermediate in the synthesis of pharmaceutical products and industrial polymers. 4-Hydroxyindole inhibits amyloid fibrillization and induces liver function impairment, thyroid abnormalities, and blood glucose fluctuations in mice. 4-Hydroxyindole holds potential for research in neurodegenerative diseases and metabolic disorders.

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4-Hydroxyindole Chemical Structure

4-Hydroxyindole Chemical Structure

CAS No. : 2380-94-1

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Based on 1 publication(s) in Google Scholar

Other Forms of 4-Hydroxyindole:

4-Hydroxyindole Related Antibodies

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Description

4-Hydroxyindole is a type of hydroxyindole in which the 1H-indole at position 4 is substituted by a hydroxyl group. 4-Hydroxyindole serves as an important raw material or intermediate in the synthesis of pharmaceutical products and industrial polymers. 4-Hydroxyindole inhibits amyloid fibrillization and induces liver function impairment, thyroid abnormalities, and blood glucose fluctuations in mice. 4-Hydroxyindole holds potential for research in neurodegenerative diseases and metabolic disorders[1][2][3].

In Vitro

4-Hydroxyindole (0–200 μM, 24 h) inhibits amyloid β (Aβ1-42, 5 μM) fibrillization in a dose-dependent manner, with an IC50 of approximately 85 μM[2].
Kinetic studies shows that 4-Hydroxyindole (5–50 μM, 14 days) inhibits amyloid β aggregation during the elongation phase. Amyloid fibrillization consists of three phases: nucleation (lag phase), elongation phase, and equilibrium phase. At 5 μM, Aβ1-40 fibrillization was inhibited by approximately 60%, while at 25 μM and 50 μM, fibrillization was almost completely suppressed[2].
4-Hydroxyindole (5–50 μM) dose-dependently blocks amyloid β-induced toxicity in PC12 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Hydroxyindole Related Antibodies

Cell Viability Assay[1]

Cell Line: PC12 Cells
Concentration: 5, 25, 50 μM
Incubation Time: 24 h
Result: Dose-dependently protected PC12 cells from Aβ1-40 and Aβ1-42 toxicity. The minimum concentration of 4-Hydroxyindole required to significantly inhibit Aβ1-40 was 2.5 μM, while the toxic effects of both Aβ1-40 and Aβ1-42 were completely blocked at 50 μM 4-Hydroxyindole.
In Vivo

4-Hydroxyindole (620 mg/kg) and its oxidation product (320 mg/kg) (intracranial injection, single-dose administration) induces liver function impairment, thyroid abnormalities, and blood glucose fluctuations in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR Albino mice, 25-30 g
Dosage: 620 mg/kg (4-Hydroxyindole), 320 mg/kg (Oxidation product of 4-Hydroxyindole)
Administration: Intracranial injection, Single dose
Result: In mice, the LD50 was 620 mg/kg (intracranial injection), showing lower toxicity than its oxidation product (LD50 was 320 mg/kg).
Oxidation product of 4-Hydroxyindole induced behavioral abnormalities in mice and reduced blood glucose, cholesterol, and blood urea nitrogen (BUN) levels while increasing liver function enzymes (ALT, AST) and alkaline phosphatase (ALP) levels.
Molecular Weight

133.15

Formula

C8H7NO
CAS No.
Appearance

Solid

SMILES

OC1=CC=CC2=C1C=CN2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 15.71 mg/mL (117.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1.43 mg/mL (10.74 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.5103 mL 37.5516 mL 75.1033 mL
5 mM 1.5021 mL 7.5103 mL 15.0207 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.57 mg/mL (11.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.57 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.57 mg/mL (11.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.57 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

Data Sheet (280 KB) SDS (393 KB)

COA (254 KB) HNMR (129 KB) RP-HPLC (96 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 7.5103 mL 37.5516 mL 75.1033 mL 187.7582 mL
5 mM 1.5021 mL 7.5103 mL 15.0207 mL 37.5516 mL
10 mM 0.7510 mL 3.7552 mL 7.5103 mL 18.7758 mL
DMSO 15 mM 0.5007 mL 2.5034 mL 5.0069 mL 12.5172 mL
20 mM 0.3755 mL 1.8776 mL 3.7552 mL 9.3879 mL
25 mM 0.3004 mL 1.5021 mL 3.0041 mL 7.5103 mL
30 mM 0.2503 mL 1.2517 mL 2.5034 mL 6.2586 mL
40 mM 0.1878 mL 0.9388 mL 1.8776 mL 4.6940 mL
50 mM 0.1502 mL 0.7510 mL 1.5021 mL 3.7552 mL
60 mM 0.1252 mL 0.6259 mL 1.2517 mL 3.1293 mL
80 mM 0.0939 mL 0.4694 mL 0.9388 mL 2.3470 mL
100 mM 0.0751 mL 0.3755 mL 0.7510 mL 1.8776 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Hydroxyindole Related Classifications

Keywords:

4-Hydroxyindole2380-94-1Biochemical Assay ReagentsAmyloid-ββ-amyloid peptideAβAbetaPC12 cellsAβ1-42Aβ1-40ICR Albino miceInhibitorinhibitorinhibit

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