2. Membrane Transporter/Ion Channel Neuronal Signaling Anti-infection Apoptosis Autophagy JAK/STAT Signaling Stem Cell/Wnt Cell Cycle/DNA Damage Protein Tyrosine Kinase/RTK PI3K/Akt/mTOR Metabolic Enzyme/Protease
  3. TRP Channel Bacterial Apoptosis Autophagy STAT PERK EGFR PI3K Akt mTOR Caspase MMP
  4. 8-Gingerol

8-Gingerol can be found in the rhizome of ginger (Z. officinale) and has oral bioactivity. It activates TRPV1, with an EC50 value of 5.0 µM. 8-Gingerol inhibits COX-2 and also suppresses the growth of H. pylori in vitro. Additionally, 8-Gingerol exhibits anticancer, antioxidant, and anti-inflammatory properties by inhibiting the epidermal growth factor receptor (EGFR) and modulating its downstream STAT3/ERK pathway to suppress the proliferation, migration, and invasion of colorectal cancer cells. 8-Gingerol also exerts immunosuppressive effects by inhibiting oxidative stress, inducing cell cycle arrest, promoting apoptosis, and regulating autophagy. Furthermore, 8-Gingerol has cardioprotective effects. 8-Gingerol is promising for research in the fields of cancer, infection, immunosuppression, and cardiovascular diseases.

For research use only. We do not sell to patients.

8-Gingerol Chemical Structure

8-Gingerol Chemical Structure

CAS No. : 23513-08-8

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Based on 3 publication(s) in Google Scholar

Other Forms of 8-Gingerol:

8-Gingerol Related Antibodies

Top Publications Citing Use of Products

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TRPC TRPM TRPV TRPA TRPML

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STAT3 STAT5 STAT6 STAT1

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Akt Akt1 Akt2 Akt3

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mTOR mTORC1 mTORC2

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

8-Gingerol can be found in the rhizome of ginger (Z. officinale) and has oral bioactivity. It activates TRPV1, with an EC50 value of 5.0 µM. 8-Gingerol inhibits COX-2 and also suppresses the growth of H. pylori in vitro. Additionally, 8-Gingerol exhibits anticancer, antioxidant, and anti-inflammatory properties by inhibiting the epidermal growth factor receptor (EGFR) and modulating its downstream STAT3/ERK pathway to suppress the proliferation, migration, and invasion of colorectal cancer cells. 8-Gingerol also exerts immunosuppressive effects by inhibiting oxidative stress, inducing cell cycle arrest, promoting apoptosis, and regulating autophagy. Furthermore, 8-Gingerol has cardioprotective effects. 8-Gingerol is promising for research in the fields of cancer, infection, immunosuppression, and cardiovascular diseases.

IC50 & Target

MMP-9

 

Caspase-9

 

In Vitro

8-Gingerol (0-70 µM, 24-72 hours) can inhibit the proliferation of HCT116 and DLD1 colorectal cancer cells, induce G0/G1 phase cell cycle arrest, and significantly promote apoptosis in HCT116 cells[3].
8-Gingerol (0-70 µM, 48 hours) significantly inhibits the migration and invasion abilities of the cells, and its effect is dependent on the EGFR/STAT3/ERK pathway[3].
8-Gingerol (100 µM, 48 hours) can reduce the effective concentration of 5-FU in HCT116 and DLD1 colorectal cancer cells, and in combination treatment, it may help reduce the toxicity of 5-FU[3].
8-Gingerol (40, 80 µg/mL, 24 hours) significantly inhibits the LPS (HY-D1056) and Concanavalin A (HY-P2149) induced splenocyte proliferation, exhibiting immunosuppressive effects[4].
8-Gingerol (10, 20 µM, 48 hours) inhibits autophagy and apoptosis in myocardial fibrosis by regulating the PI3K/Akt/mTOR signaling pathway, showing significant cardioprotective effects[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

8-Gingerol Related Antibodies

Cell Viability Assay[3]

Cell Line: HCT116 (colorectal cancer), DLD1 (colorectal cancer)
Concentration: 0, 10, 30, 50, 70 µM
Incubation Time: 24h, 48h, 72h
Result: Dose-dependently inhibited HCT116 and DLD1 cell proliferation, with IC50 values of HCT116: 77.4 µM (48h), DLD1: 53.7 µM (48h)

Cell Cycle Analysis[3]

Cell Line: HCT116 (colorectal cancer), DLD1 (colorectal cancer)
Concentration: 0, 10, 30, 50, 70 µM
Incubation Time: 48h
Result: Significantly induced G0/G1 cell cycle arrest in HCT116 and DLD1 cells.

Apoptosis Analysis[3]

Cell Line: HCT116 (colorectal cancer), DLD1 (colorectal cancer)
Concentration: 0, 10, 30, 50, 70 µM
Incubation Time: 48h
Result: Dose-dependently increased the apoptosis rate in HCT116 cells, but no significant change in DLD1 cells.

Western Blot Analysis[5]

Cell Line: Cardiac cells from ISO-induced myocardial fibrosis mouse model
Concentration: 10, 20 µM
Incubation Time: 48h
Result: Significantly down-regulated the activity of MMP-9, Caspase-9, and Bax proteins, significantly up-regulated the activity of Bcl-2 protein, and inhibited apoptosis.
In Vivo

8-Gingerol (50, 100 mg/kg, i.p., once daily for 7 days) can suppress the humoral and cellular immune responses in mice, likely by directly inhibiting activated T cells and B cells[4].
8-Gingerol (10, 20 mg/kg, i.p., once daily for 14 days) improves ISO-induced myocardial fibrosis in mice by regulating the PI3K/Akt/mTOR signaling pathway, inhibiting oxidative stress, apoptosis, and autophagy[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: OVA-immunized mice (BALB/c male mice, 5 weeks old, 18-22g, immunized with ovalbumin (OVA))[4]
Dosage: 25, 50, 100 mg/kg
Administration: Intraperitoneal injection (i.p.), once daily for 7 consecutive days
Result: Significantly suppressed LPS-, ConA-, and OVA-induced splenocyte proliferation in OVA-immunized mice and significantly reduced the percentage of CD19+ B cells and CD3+ T cells at high doses (50, 100 mg/kg)
Animal Model: Isoproterenol (ISO)-induced myocardial fibrosis (MF) mouse model, Kunming mice (male, 6-8 weeks old, 15-20g)[5]
Dosage: 10 mg/kg (low dose), 20 mg/kg (high dose)
Administration: Intraperitoneal injection (i.p.), once daily for 14 days
Result: Significantly reduced the heart weight, left ventricle weight index, CK, LDH levels, ROS generation, and pathological heart damage induced by ISO.
Molecular Weight

322.44

Formula

C19H30O4
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

CCCCCCC[C@H](O)CC(CCC1=CC=C(O)C(OC)=C1)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (310.14 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1014 mL 15.5068 mL 31.0135 mL
5 mM 0.6203 mL 3.1014 mL 6.2027 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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% 
+
%
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%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.82%

SDS (252 KB)

COA (242 KB) HNMR (273 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1014 mL 15.5068 mL 31.0135 mL 77.5338 mL
5 mM 0.6203 mL 3.1014 mL 6.2027 mL 15.5068 mL
10 mM 0.3101 mL 1.5507 mL 3.1014 mL 7.7534 mL
15 mM 0.2068 mL 1.0338 mL 2.0676 mL 5.1689 mL
20 mM 0.1551 mL 0.7753 mL 1.5507 mL 3.8767 mL
25 mM 0.1241 mL 0.6203 mL 1.2405 mL 3.1014 mL
30 mM 0.1034 mL 0.5169 mL 1.0338 mL 2.5845 mL
40 mM 0.0775 mL 0.3877 mL 0.7753 mL 1.9383 mL
50 mM 0.0620 mL 0.3101 mL 0.6203 mL 1.5507 mL
60 mM 0.0517 mL 0.2584 mL 0.5169 mL 1.2922 mL
80 mM 0.0388 mL 0.1938 mL 0.3877 mL 0.9692 mL
100 mM 0.0310 mL 0.1551 mL 0.3101 mL 0.7753 mL
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8-Gingerol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-Gingerol23513-08-8TRP ChannelBacterialApoptosisAutophagySTATPERKEGFRPI3KAktmTORCaspaseMMPTransient receptor potential channelsProtein kinase R-like endoplasmic reticulum kinasePKR-like endoplasmic reticulum kinaseEpidermal growth factor receptorErbB-1HER1Phosphoinositide 3-kinasePKBProtein kinase BMammalian target of RapamycinMatrix metalloproteinasesOVA-immunized miceIsoproterenol (ISO)-induced myocardial fibrosis (MF) modelHCT116colorectal cancerDLD1Anti-cancerantioxidantanti-inflammatoryInhibitorinhibitorinhibit

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