2. Apoptosis
  3. Apoptosis
  4. Minnelide

Minnelide is a prodrug of triptolide that shows potent antitumor activity in a number of tumor types, particularly in pancreatic cancer. Minnelide promotes apoptosis.

For research use only. We do not sell to patients.

Minnelide Chemical Structure

Minnelide Chemical Structure

CAS No. : 1254702-87-8

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Minnelide:

Minnelide Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Minnelide is a prodrug of triptolide that shows potent antitumor activity in a number of tumor types, particularly in pancreatic cancer. Minnelide promotes apoptosis[1].

In Vitro

Minnelide (0-200 nM; 48 hours) shows significantly decreased cell viability in pancreatic cancer cell lines after treatment in the presence, but not in the absence, of phosphatase[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Minnelide Related Antibodies

Cell Viability Assay[2]

Cell Line: Pancreatic cancer cell line: S2-013, MIA PaCa-2, S2-VP10, and Panc-1 cells
Concentration: 0.100 nM, 200 nM
Incubation Time: 48 hours
Result: Decreased cell viability of in vitro.
In Vivo

Minnelide (injection intraperitoneally; 0.1-0.6 mg/kg; once daily or twice daily) leads to a marked decrease in tumor weight and volume at the end of treatment and increases survival in orthotopic model of pancreatic cancer with MIA PaCa-2–derived human pancreatic tumors[2].
Minnelide (injection intraperitoneally; 0.42 mg/kg; once daily; 28 days) prevents locoregional spread and leads to a decrease in average tumor weight in a xenograft model of pancreatic cancer with metastatic S2-013 cells[2].
Minnelide (injection intraperitoneally; 0.42 mg/kg, 0.21 mg/kg; once daily) causes tumor regression and tumors from Minnelide-treated animals showed fibrosis and the presence of pyknotic nuclei in human pancreatic cancer xenografts in SCID mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Orthotopic model of pancreatic cancer with MIA PaCa 2-derived human pancreatic tumors in athymic nude mice[2]
Dosage: 0.1-0.6 mg/kg
Administration: Injection intraperitoneally; 0.1-0.6 mg/kg; once daily or twice daily
Result: Prevented pancreatic tumor growth in vivo.
Animal Model: Xenograft model of pancreatic cancer with metastatic S2-013 cell line in athymic nude mice[2]
Dosage: 0.42 mg/kg
Administration: Injection intraperitoneally; 0.42 mg/kg; once daily
Result: Prevented extensive spread from the primary site of injection.
Animal Model: Human pancreatic cancer xenografts in SCID mice[2]
Dosage: 0.21 mg/kg, 0.42 mg/kg
Administration: Injection intraperitoneally; 0.42 mg/kg; once daily
Result: Reduced tumor burden in human xenografts from patients.
Clinical Trial
Molecular Weight

514.37

Formula

C21H25Na2O10P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@@]34[C@]([C@@H]5C[C@@]1([H])C6=C(C(OC6)=O)CC2)(O5)[C@@H]([C@](O7)(C(C)C)[C@@H]7[C@@H]3O4)OCOP(O[Na])(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 93.33 mg/mL (181.45 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (32.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9441 mL 9.7206 mL 19.4413 mL
5 mM 0.3888 mL 1.9441 mL 3.8883 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (3.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.67 mg/mL (3.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.91%

COA (266 KB) HNMR (146 KB) RP-HPLC (169 KB) LCMS (381 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.9441 mL 9.7206 mL 19.4413 mL 48.6031 mL
5 mM 0.3888 mL 1.9441 mL 3.8883 mL 9.7206 mL
10 mM 0.1944 mL 0.9721 mL 1.9441 mL 4.8603 mL
15 mM 0.1296 mL 0.6480 mL 1.2961 mL 3.2402 mL
20 mM 0.0972 mL 0.4860 mL 0.9721 mL 2.4302 mL
25 mM 0.0778 mL 0.3888 mL 0.7777 mL 1.9441 mL
30 mM 0.0648 mL 0.3240 mL 0.6480 mL 1.6201 mL
H2O 40 mM 0.0486 mL 0.2430 mL 0.4860 mL 1.2151 mL
50 mM 0.0389 mL 0.1944 mL 0.3888 mL 0.9721 mL
60 mM 0.0324 mL 0.1620 mL 0.3240 mL 0.8101 mL
80 mM 0.0243 mL 0.1215 mL 0.2430 mL 0.6075 mL
100 mM 0.0194 mL 0.0972 mL 0.1944 mL 0.4860 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Minnelide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Minnelide1254702-87-8ApoptosisInhibitorinhibitorinhibit

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