NCK1 - NCK adaptor protein 1 Gene

Homo sapiens

Also known as NCK; nck-1; NCKalpha

Gene ID: 4690 | Gene type: protein coding

About NCK1

Cytogenetic location: 3q22.3 Genomic coordinates (GRCh38): 3:136,862,208-136,951,606 (from NCBI)

This gene has 12 transcripts (splice variants), 198 orthologues and 9 paralogues. Ubiquitous expression in lymph node (RPKM 10.1), fat (RPKM 9.3) and 25 other tissues.

Summary

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from Receptor Tyrosine Kinases to downstream signal recipients such as Ras. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]

NCK1 Products(3)

mRNA	Protein	Name
NM_001190796.3	NP_001177725.1	cytoplasmic protein NCK1 isoform 2
NM_001291999.2	NP_001278928.1	cytoplasmic protein NCK1 isoform 1
NM_006153.6	NP_006144.1	cytoplasmic protein NCK1 isoform 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Reference	Source
enables eukaryotic initiation factor eIF2 binding	IPI IPI: Inferred from physical interaction	11959995	GOA
enables molecular condensate scaffold activity	IDA IDA: Inferred from direct assay	26553976	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	9346925	GOA
enables protein kinase inhibitor activity	IDA IDA: Inferred from direct assay	18835251	GOA
enables protein-macromolecule adaptor activity	IDA IDA: Inferred from direct assay	21707536	GOA
enables receptor tyrosine kinase binding	IPI IPI: Inferred from physical interaction	23358419	GOA
enables signaling adaptor activity	IDA IDA: Inferred from direct assay	16835242	GOA
enables signaling receptor binding	IPI IPI: Inferred from physical interaction	12110186	GOA

Biological Process GO Annotation	Evidence	Reference	Source
involved in T cell activation	IMP IMP: Inferred from mutant phenotype	12110186	GOA
involved in antiviral innate immune response	IDA IDA: Inferred from direct assay	18835251	GOA
involved in negative regulation of PERK-mediated unfolded protein response	IDA IDA: Inferred from direct assay	14676213	GOA
involved in negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation	IDA IDA: Inferred from direct assay	14676213	GOA
involved in negative regulation of insulin receptor signaling pathway	IMP IMP: Inferred from mutant phenotype	21707536	GOA
involved in negative regulation of peptidyl-serine phosphorylation	IDA IDA: Inferred from direct assay	14676213	GOA
involved in negative regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	16835242	GOA
involved in positive regulation of T cell proliferation	IMP IMP: Inferred from mutant phenotype	12110186	GOA
involved in positive regulation of actin filament polymerization	IMP IMP: Inferred from mutant phenotype	12110186	GOA
involved in positive regulation of cap-dependent translational initiation	IDA IDA: Inferred from direct assay	11959995	GOA
involved in positive regulation of cap-independent translational initiation	IDA IDA: Inferred from direct assay	11959995	GOA
involved in positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway	IDA IDA: Inferred from direct assay	14676213	GOA
involved in positive regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	10026169	GOA
involved in positive regulation of translation in response to endoplasmic reticulum stress	IDA IDA: Inferred from direct assay	14676213	GOA
involved in response to endoplasmic reticulum stress	IDA IDA: Inferred from direct assay	16835242	GOA

Cellular Component GO Annotation	Evidence	Reference	Source
located in cytoplasm	IDA IDA: Inferred from direct assay	25468996	GOA
located in endoplasmic reticulum	IDA IDA: Inferred from direct assay	16835242	GOA
part of protein phosphatase type 1 complex	IDA IDA: Inferred from direct assay	16835242	GOA
located in ribosome	IDA IDA: Inferred from direct assay	11959995	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

NCK1 Protein Structure

SH3_1

SH3_9

SH3_1

SH2

0
100
200
300
377 a.a.

Protein Preferred Names

Protein Names

cytoplasmic protein NCK1

NCK tyrosine kinase

NCK1 Protein-protein interaction Information

Type Cross Intra	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	Reference
Intra	NCK1	P16333	KDR	Homo sapiens	P35968	Anti Tag CoIP	16966330
Intra	NCK1	P16333	CHAF1A	Homo sapiens	Q13111	Peptide Array	17474147
Intra	NCK1	P16333	RASA1	Homo sapiens	P20936	Anti Bait CoIP	21664272
Intra	NCK1	P16333	RASA1	Homo sapiens	P20936	SLCA	21664272
Intra	NCK1	P16333	TPX2	Homo sapiens	Q9ULW0	Peptide Array	17474147
Intra	NCK1	P16333	MET	Homo sapiens	P08581	FPS	24728074
Intra	NCK1	P16333	PAK2	Homo sapiens	Q13177	Filter Binding	16374509
Intra	NCK1	P16333	PAK2	Homo sapiens	Q13177	Phage Display	16374509
Intra	NCK1	P16333	PAK2	Homo sapiens	Q13177	Validated Y2H	32296183
Intra	NCK1	P16333	PAK2	Homo sapiens	Q13177	Anti Tag CoIP	33961781
Intra	NCK1	P16333	TNIK	Homo sapiens	Q9UKE5	Anti Tag CoIP	33961781
Intra	NCK1	P16333	ABL2	Homo sapiens	P42684	PLA	25241761
Intra	NCK1	P16333	ABL2	Homo sapiens	P42684	Anti Tag CoIP	33961781
Intra	NCK1	P16333	DAB2	Homo sapiens	P98082	Peptide Array	17474147
Intra	NCK1	P16333	PIK3AP1	Homo sapiens	Q6ZUJ8-3	Validated Y2H	32296183
Intra	NCK1	P16333	DLGAP4	Homo sapiens	Q9Y2H0-1	Validated Y2H	32296183
Intra	NCK1	P16333	CD3E	Homo sapiens	P07766	ELISA	18955169
Intra	NCK1	P16333	CD3E	Homo sapiens	P07766	NMR	18955169
Intra	NCK1	P16333	KIT	Homo sapiens	P10721	FPS	24728074
Intra	NCK1	P16333	SHANK2	Homo sapiens	Q9UPX8	Peptide Array	17474147
Intra	NCK1	P16333	AGER	Homo sapiens	Q15109	Peptide Array	17474147
Intra	NCK1	P16333	ASB16	Homo sapiens	Q96NS5	Peptide Array	17474147
Intra	NCK1	P16333	SHANK3	Homo sapiens	Q9BYB0	Y2H	21988832
Intra	NCK1	P16333	NCKAP5	Homo sapiens	O14513	Peptide Array	17474147
Intra	NCK1	P16333	AIRE	Homo sapiens	O43918	Peptide Array	17474147
Intra	NCK1	P16333	FYB1	Homo sapiens	O15117	Peptide Array	17474147
Intra	NCK1	P16333	MEPE	Homo sapiens	Q9NQ76	Peptide Array	17474147
Intra	NCK1	P16333	SEMA7A	Homo sapiens	O75326	Peptide Array	17474147
Intra	NCK1	P16333	PHACTR2	Homo sapiens	O75167	Peptide Array	17474147
Intra	NCK1	P16333	RAG1	Homo sapiens	P15918	Peptide Array	17474147
Intra	NCK1	P16333	DAG1	Homo sapiens	Q14118	Peptide Array	17474147
Intra	NCK1	P16333	TRAIP	Homo sapiens	Q9BWF2	Peptide Array	17474147
Intra	NCK1	P16333	RIMS2	Homo sapiens	Q9UQ26	Peptide Array	17474147
Intra	NCK1	P16333	TULP1	Homo sapiens	O00294	Peptide Array	17474147
Intra	NCK1	P16333	ELK3	Homo sapiens	P41970	Peptide Array	17474147
Intra	NCK1	P16333	SLC23A1	Homo sapiens	Q9UHI7	Peptide Array	17474147
Intra	NCK1	P16333	TPSAB1	Homo sapiens	Q15661	Peptide Array	17474147
Intra	NCK1	P16333	ABCG5	Homo sapiens	Q9H222	Peptide Array	17474147
Intra	NCK1	P16333	NHSL2	Homo sapiens	Q5HYW2	Validated Y2H	32296183
Intra	NCK1	P16333	GHR	Homo sapiens	P10912	PLA	25241761
Intra	NCK1	P16333	EGFR	Homo sapiens	P00533	CoIP	8662998
Intra	NCK1	P16333	EGFR	Homo sapiens	P00533	Ub Reconstruction	24658140
Intra	NCK1	P16333	SOS1	Homo sapiens	Q07889	Pull Down	10206341
Intra	NCK1	P16333	SOS1	Homo sapiens	Q07889	Anti Tag CoIP	33961781
Intra	NCK1	P16333	SOS1	Homo sapiens	Q07889	Peptide Array	17474147
Intra	NCK1	P16333	SOS2	Homo sapiens	Q07890	Anti Tag CoIP	33961781
Intra	NCK1	P16333	SOS2	Homo sapiens	Q07890	Peptide Array	17474147
Intra	NCK1	P16333	SP1	Homo sapiens	P08047	Peptide Array	17474147
Intra	NCK1	P16333	PNMA2	Homo sapiens	Q9UL42	Peptide Array	17474147
Intra	NCK1	P16333	CELSR3	Homo sapiens	Q9NYQ7	Peptide Array	17474147
Intra	NCK1	P16333	SYNJ2	Homo sapiens	O15056	Peptide Array	17474147
Intra	NCK1	P16333	ASAP2	Homo sapiens	O43150	Anti Tag CoIP	33961781
Intra	NCK1	P16333	ASAP2	Homo sapiens	O43150	Peptide Array	17474147
Intra	NCK1	P16333	WIPF1	Homo sapiens	O43516	Anti Tag CoIP	33961781
Intra	NCK1	P16333	WIPF1	Homo sapiens	O43516	Pull Down	12620186
Intra	NCK1	P16333	ASAP1	Homo sapiens	Q9ULH1	Peptide Array	17474147
Intra	NCK1	P16333	LCP2	Homo sapiens	Q13094	Solution Sedimentation	20562827
Intra	NCK1	P16333	LCP2	Homo sapiens	Q13094	Anti Bait CoIP	20562827
Intra	NCK1	P16333	LCP2	Homo sapiens	Q13094	ITC	20562827
Intra	NCK1	P16333	LCP2	Homo sapiens	Q13094	FRET	20562827
Intra	NCK1	P16333	NELFB	Homo sapiens	Q8WX92	Peptide Array	17474147
Intra	NCK1	P16333	FLNB	Homo sapiens	O75369	Peptide Array	17474147
Intra	NCK1	P16333	H1-4	Homo sapiens	P10412	Peptide Array	17474147
Intra	NCK1	P16333	ABI1	Homo sapiens	Q8IZP0	Anti Tag CoIP	33961781
Intra	NCK1	P16333	ABI1	Homo sapiens	Q8IZP0	Pull Down	11418237
Intra	NCK1	P16333	NHSL3	Homo sapiens	Q9P206	Anti Tag CoIP	33961781
Intra	NCK1	P16333	TMPO	Homo sapiens	P42167	Peptide Array	17474147
Intra	NCK1	P16333	STK38	Homo sapiens	Q15208	Anti Tag CoIP	31980649
Intra	NCK1	P16333	GAB1	Homo sapiens	Q13480	FPS	24728074
Intra	NCK1	P16333	PIK3C2B	Homo sapiens	O00750	Peptide Array	17474147
Intra	NCK1	P16333	PTPN4	Homo sapiens	P29074	Peptide Array	17474147
Intra	NCK1	P16333	DNM1	Homo sapiens	Q05193	Anti Bait CoIP	10206341
Intra	NCK1	P16333	DNM1	Homo sapiens	Q05193	Pull Down	10206341
Intra	NCK1	P16333	CHN2	Homo sapiens	P52757	Validated Y2H	32296183
Intra	NCK1	P16333	DLGAP4	Homo sapiens	Q9Y2H0	Peptide Array	17474147
Intra	NCK1	P16333	TRIM39	Homo sapiens	Q9HCM9	Peptide Array	17474147
Intra	NCK1	P16333	GAD1	Homo sapiens	Q99259	Peptide Array	17474147
Intra	NCK1	P16333	CBLB	Homo sapiens	Q13191	Anti Tag CoIP	25218436
Intra	NCK1	P16333	GSTZ1	Homo sapiens	O43708	Peptide Array	17474147
Intra	NCK1	P16333	ISG20L2	Homo sapiens	Q9H9L3	Peptide Array	17474147
Intra	NCK1	P16333	NR5A1	Homo sapiens	Q13285	Phage Display	39009827
Intra	NCK1	P16333	WASL	Homo sapiens	O00401	Anti Tag CoIP	33961781
Intra	NCK1	P16333	WASL	Homo sapiens	O00401	Anti Tag CoIP	26496610
Intra	NCK1	P16333	CDC27	Homo sapiens	P30260	Peptide Array	17474147
Intra	NCK1	P16333	NPHS1	Homo sapiens	O60500	Pull Down	16525419
Intra	NCK1	P16333	NPHS1	Homo sapiens	O60500	Anti Bait CoIP	16525419

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant NCK1 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P70304	NCK1 Protein, Human (His)	P16333-1 (M1-S377)	≥95%

Related Diseases

Diseases

Alias

Wiskott-Aldrich Syndrome

WAS	Eczema-Thrombocytopenia-Immunodeficiency Syndrome
Immunodeficiency 2	Aldrich Syndrome
Imd2	Wiskott-Aldrich Syndrome 1
Was1	Wiskott Syndrome
Wiskott Aldrich Syndrome	Eczema Thrombocytopenia Immunodeficiency Syndrome
Imd 2

Vaccinia

Agammaglobulinemia 4, Autosomal Recessive

Agammaglobulinemia 4	AGM4
Agammaglobulinemia, Autosomal Recessive, Due To Blnk Defect	B Cell Linker Protein Deficiency
B-Cell Linker Protein Deficiency	Blnk Deficiency
Agammaglobulinemia Autosomal Recessive Due To Blnk Defect	Agammaglobulinemia, Type 4, Autosomal Recessive

Focal Segmental Glomerulosclerosis

Familial Idiopathic Steroid-Resistant Nephrotic Syndrome	Focal Glomerulosclerosis
Fsgs	Segmental Glomerulosclerosis
Glomerulosclerosis, Focal Segmental	Fgs
Focal Glomerular Sclerosis	Familial Idiopathic Nephrotic Syndrome
Focal Sclerosis With Hyalinosis	Glomerulosclerosis, Focal
Glomerulosclerosis Focal	Glomerulosclerosis, Segmental, Focal
Focal Segmental Glomerulosclerosis, Not Otherwise Specified

Familial Nephrotic Syndrome

Congenital Nephrotic Syndrome	Nephrosis, Congenital
Finnish Congenital Nephrotic Syndrome

Frank-Ter Haar Syndrome

Ter Haar Syndrome	Borrone Dermatocardioskeletal Syndrome
FTHS	Autosomal Recessive Melnick-Needles Syndrome
Borrone Di Rocco Crovato Syndrome	Megalocornea, Multiple Skeletal Anomalies, And Developmental Delay
Melnick-Needles Syndrome, Autosomal Recessive, Formerly	Frank Ter Haar Syndrome

Melanoma

Malignant Melanoma	Cutaneous Melanoma
Naevocarcinoma	Malignant Melanomas

Neutropenia, Severe Congenital, X-Linked

X-Linked Severe Congenital Neutropenia	XLN
SCNX	Severe Congenital Neutropenia X-Linked
Neutropenia, Congenital, Severe, X-Linked

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS09080	NCK1 Human Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	NCK1	MGD	MGI:109601
Rattus norvegicus	NCK1	RGD	RGD:1310688
Canis familiaris	NCK1	VGNC	VGNC:52892
Bos taurus	NCK1	VGNC	VGNC:52804
Felis catus	NCK1	VGNC	VGNC:68427
Macaca mulatta	NCK1	VGNC	VGNC:110350
Others	NCK1	NCBI

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