2. Gene
  3. RNF8 - ring finger protein 8 Gene

RNF8 - ring finger protein 8 Gene

  • Gene
  • Protein
  • Disease
  • Agent
  • Ortholog
Homo sapiens

Also known as hRNF8

Gene ID: 9025 | Gene type: protein coding

About RNF8

Cytogenetic location: 6p21.2 Genomic coordinates (GRCh38): 6:37,353,983-37,394,734 (from NCBI)

This gene has 8 transcripts (splice variants) and 192 orthologues. Ubiquitous expression in testis (RPKM 6.4), brain (RPKM 2.8) and 24 other tissues.

Summary

The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating Enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin Ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

RNF8 Products(2)

mRNA Protein Name
NM_003958.4 NP_003949.1 E3 ubiquitin-protein ligase RNF8 isoform 1
NM_183078.3 NP_898901.1 E3 ubiquitin-protein ligase RNF8 isoform 2
Gene Ontology
  • Molecular Function
  • Biological Process
  • Cellular Component
Molecular Function GO Annotation Evidence Reference Source
enables chromatin binding IDA
IDA: Inferred from direct assay
18001824 GOA
enables histone binding IDA
IDA: Inferred from direct assay
18001824 GOA
enables identical protein binding IPI
IPI: Inferred from physical interaction
22980979 GOA
enables protein binding IPI
IPI: Inferred from physical interaction
16189514 GOA
enables protein homodimerization activity IDA
IDA: Inferred from direct assay
22980979 GOA
enables ubiquitin protein ligase activity IDA
IDA: Inferred from direct assay
18001824 GOA
enables ubiquitin protein ligase binding IPI
IPI: Inferred from physical interaction
22266820 GOA
enables zinc ion binding IDA
IDA: Inferred from direct assay
22980979 GOA
Biological Process GO Annotation Evidence Reference Source
involved in DNA damage response IDA
IDA: Inferred from direct assay
20550933 GOA
involved in DNA repair-dependent chromatin remodeling IDA
IDA: Inferred from direct assay
18001824 GOA
involved in double-strand break repair IDA
IDA: Inferred from direct assay
18001824 GOA
involved in epigenetic regulation of gene expression IMP
IMP: Inferred from mutant phenotype
20550933 GOA
involved in negative regulation of transcription elongation by RNA polymerase II IMP
IMP: Inferred from mutant phenotype
20550933 GOA
involved in positive regulation of DNA repair IDA
IDA: Inferred from direct assay
18001824 GOA
involved in positive regulation of double-strand break repair via homologous recombination IDA
IDA: Inferred from direct assay
23115235 GOA
involved in protein K48-linked ubiquitination IDA
IDA: Inferred from direct assay
21911360 GOA
involved in protein K6-linked ubiquitination IDA
IDA: Inferred from direct assay
23115235 GOA
involved in protein K63-linked ubiquitination IDA
IDA: Inferred from direct assay
21911360 GOA
involved in protein autoubiquitination IDA
IDA: Inferred from direct assay
22266820 GOA
involved in response to ionizing radiation IDA
IDA: Inferred from direct assay
18001824 GOA
involved in ubiquitin-dependent protein catabolic process IDA
IDA: Inferred from direct assay
22266820 GOA
Cellular Component GO Annotation Evidence Reference Source
located in nucleus IDA
IDA: Inferred from direct assay
18001824 GOA
located in site of double-strand break IDA
IDA: Inferred from direct assay
22266820 GOA
part of ubiquitin ligase complex IDA
IDA: Inferred from direct assay
18001824 GOA
EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RNF8 Protein Structure

FHA

FHA: FHA domain (38 - 109)

zf-RING_2

zf-RING_2: Ring finger domain (402 - 441)

  • 0
  • 100
  • 200
  • 300
  • 400
  • 485 a.a.
Protein Preferred Names Protein Names

E3 ubiquitin-protein ligase RNF8

C3HC4-type zinc finger protein

RNF8 Protein-protein interaction Information

Type
Protein Name Protein ID Interactor Interactor Species Interactor ID Detection Method Reference
Intra
RNF8 O76064 KRTAP10-8 Homo sapiens P60410
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 KRTAP10-8 Homo sapiens P60410
Validated Y2H
32296183
Intra
RNF8 O76064 KRTAP10-8 Homo sapiens P60410
Y2H Array
32296183
Intra
RNF8 O76064 HOMEZ Homo sapiens Q8IX15-3
Validated Y2H
25416956
Intra
RNF8 O76064 FAM9B Homo sapiens Q8IZU0
Validated Y2H
32296183
Intra
RNF8 O76064 KIF24 Homo sapiens Q5T7B8-2
Y2H Array
32296183
Intra
RNF8 O76064 KIF24 Homo sapiens Q5T7B8-2
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 KRTAP9-2 Homo sapiens Q9BYQ4
Validated Y2H
32296183
Intra
RNF8 O76064 PGRMC2 Homo sapiens O15173
Y2H Array
32296183
Intra
RNF8 O76064 PGRMC2 Homo sapiens O15173
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 TOMM20L Homo sapiens Q6UXN7
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 TOMM20L Homo sapiens Q6UXN7
Validated Y2H
32296183
Intra
RNF8 O76064 TOMM20L Homo sapiens Q6UXN7
Y2H Array
32296183
Intra
RNF8 O76064 KRTAP9-8 Homo sapiens Q9BYQ0
Validated Y2H
32296183
Intra
RNF8 O76064 RBFOX2 Homo sapiens O43251-10
Y2H Array
32296183
Intra
RNF8 O76064 RBFOX2 Homo sapiens O43251-10
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 ZBTB10 Homo sapiens Q96DT7-3
Validated Y2H
32296183
Intra
RNF8 O76064 ZBTB10 Homo sapiens Q96DT7-3
Y2H Array
32296183
Intra
RNF8 O76064 ZBTB10 Homo sapiens Q96DT7-3
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 CCDC14 Homo sapiens Q49A88-3
Validated Y2H
32296183
Intra
RNF8 O76064 EPN2 Homo sapiens O95208-2
Y2H Array
32296183
Intra
RNF8 O76064 EPN2 Homo sapiens O95208-2
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 EPN2 Homo sapiens O95208-2
Validated Y2H
32296183
Intra
RNF8 O76064 CEP85 Homo sapiens Q6P2H3-3
Y2H Array
32296183
Intra
RNF8 O76064 CEP85 Homo sapiens Q6P2H3-3
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 CEP85 Homo sapiens Q6P2H3-3
Validated Y2H
32296183
Intra
RNF8 O76064 EPN3 Homo sapiens I6L9I8
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 EPN3 Homo sapiens I6L9I8
Y2H Array
32296183
Intra
RNF8 O76064 SCN3B Homo sapiens Q9NY72
Validated Y2H
32296183
Intra
RNF8 O76064 SCN3B Homo sapiens Q9NY72
Y2H Array
32296183
Intra
RNF8 O76064 SCN3B Homo sapiens Q9NY72
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 VRK1 Homo sapiens Q99986
Anti Tag CoIP
26869104
Intra
RNF8 O76064 ZNF346 Homo sapiens Q9UL40
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 ZNF346 Homo sapiens Q9UL40
Validated Y2H
32296183
Intra
RNF8 O76064 ZNF346 Homo sapiens Q9UL40
Y2H Array
32296183
Intra
RNF8 O76064 KANK2 Homo sapiens Q63ZY3
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 KANK2 Homo sapiens Q63ZY3
Validated Y2H
32296183
Intra
RNF8 O76064 KANK2 Homo sapiens Q63ZY3
Y2H Array
32296183
Intra
RNF8 O76064 PCYT1A Homo sapiens P49585
Validated Y2H
32296183
Intra
RNF8 O76064 PCYT1A Homo sapiens P49585
Y2H Array
32296183
Intra
RNF8 O76064 PCYT1A Homo sapiens P49585
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 LMO4 Homo sapiens P61968
Validated Y2H
32296183
Intra
RNF8 O76064 PNMA2 Homo sapiens Q9UL42
Validated Y2H
32296183
Intra
RNF8 O76064 PNMA2 Homo sapiens Q9UL42
Y2H Array
32296183
Intra
RNF8 O76064 PNMA2 Homo sapiens Q9UL42
Y2H
21516116
Intra
RNF8 O76064 PNMA2 Homo sapiens Q9UL42
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 DNM2 Homo sapiens P50570
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 DNM2 Homo sapiens P50570
Validated Y2H
32296183
Intra
RNF8 O76064 DNM2 Homo sapiens P50570
Y2H Array
32296183
Intra
RNF8 O76064 UBE2D2 Homo sapiens P62837
Y2H Array
19690564
Intra
RNF8 O76064 UBE2D2 Homo sapiens P62837
Validated Y2H
32296183
Intra
RNF8 O76064 VCP Homo sapiens P55072
Y2H Prey Pooling
25416956
Intra
RNF8 O76064 VCP Homo sapiens P55072
Validated Y2H
25416956
Intra
RNF8 O76064 TNIP1 Homo sapiens Q15025
Y2H Array
32296183
Intra
RNF8 O76064 TNIP1 Homo sapiens Q15025
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 TNIP1 Homo sapiens Q15025
Validated Y2H
32296183
Intra
RNF8 O76064 PSMB1 Homo sapiens P20618
Validated Y2H
32296183
Intra
RNF8 O76064 MAD1L1 Homo sapiens Q9Y6D9
Validated Y2H
32296183
Intra
RNF8 O76064 WASL Homo sapiens O00401
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 MAD1L1 Homo sapiens Q9Y6D9
Y2H Array
25416956
Intra
RNF8 O76064 SEPTIN3 Homo sapiens Q9UH03
Y2H Prey Pooling
25416956
Intra
RNF8 O76064 MAD1L1 Homo sapiens Q9Y6D9
Y2H Array
32296183
Intra
RNF8 O76064 SEPTIN3 Homo sapiens Q9UH03
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 MAD1L1 Homo sapiens Q9Y6D9
Y2H Prey Pooling
25416956
Intra
RNF8 O76064 WASL Homo sapiens O00401
Y2H
21516116
Intra
RNF8 O76064 SEPTIN3 Homo sapiens Q9UH03
Validated Y2H
25416956
Intra
RNF8 O76064 WASL Homo sapiens O00401
Y2H Array
32296183
Intra
RNF8 O76064 MAD1L1 Homo sapiens Q9Y6D9
Validated Y2H
25416956
Intra
RNF8 O76064 MAD1L1 Homo sapiens Q9Y6D9
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 GPRASP2 Homo sapiens Q96D09
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 GPRASP2 Homo sapiens Q96D09
Y2H Array
32296183
Intra
RNF8 O76064 GPRASP2 Homo sapiens Q96D09
Validated Y2H
32296183
Intra
RNF8 O76064 MDC1 Homo sapiens Q14676
Anti Bait CoIP
18001824
Intra
RNF8 O76064 MDC1 Homo sapiens Q14676
Pull Down
18001825
Intra
RNF8 O76064 FSD2 Homo sapiens A1L4K1
Y2H Array
32296183
Intra
RNF8 O76064 FSD2 Homo sapiens A1L4K1
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 DNTTIP2 Homo sapiens Q5QJE6
Validated Y2H
32296183
Intra
RNF8 O76064 SH3GL3 Homo sapiens Q8IVP1
Validated Y2H
32296183
Intra
RNF8 O76064 SH3GL3 Homo sapiens Q8IVP1
Y2H Array
32296183
Intra
RNF8 O76064 SH3GL3 Homo sapiens Q8IVP1
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 WASHC3 Homo sapiens Q9Y3C0
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 WASHC3 Homo sapiens Q9Y3C0
Validated Y2H
32296183
Intra
RNF8 O76064 WASHC3 Homo sapiens Q9Y3C0
Y2H Array
32296183
Intra
RNF8 O76064 STX11 Homo sapiens O75558
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 STX11 Homo sapiens O75558
Y2H Array
32296183
Intra
RNF8 O76064 CCDC50 Homo sapiens Q8IVM0
Validated Y2H
32296183
Intra
RNF8 O76064 CCDC50 Homo sapiens Q8IVM0
Y2H Array
32296183
Intra
RNF8 O76064 CCDC50 Homo sapiens Q8IVM0
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 PPCDC Homo sapiens Q96CD2
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 PPCDC Homo sapiens Q96CD2
Validated Y2H
32296183
Intra
RNF8 O76064 PPCDC Homo sapiens Q96CD2
Y2H Array
32296183
Intra
RNF8 O76064 FAM118B Homo sapiens Q9BPY3
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 FAM118B Homo sapiens Q9BPY3
Validated Y2H
32296183
Intra
RNF8 O76064 FAM118B Homo sapiens Q9BPY3
Y2H Array
32296183
Intra
RNF8 O76064 SEPTIN3 Homo sapiens Q9UH03
Validated Y2H
32296183
Intra
RNF8 O76064 SEPTIN3 Homo sapiens Q9UH03
Y2H Array
32296183
Intra
RNF8 O76064 C2CD6 Homo sapiens Q53TS8
Validated Y2H
32296183
Intra
RNF8 O76064 DDIT4L Homo sapiens Q96D03
Y2H Array
32296183
Intra
RNF8 O76064 DDIT4L Homo sapiens Q96D03
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 DDIT4L Homo sapiens Q96D03
Validated Y2H
32296183
Intra
RNF8 O76064 ZNF496 Homo sapiens Q96IT1
Validated Y2H
32296183
Intra
RNF8 O76064 ZNF496 Homo sapiens Q96IT1
Y2H Array
32296183
Intra
RNF8 O76064 ZNF496 Homo sapiens Q96IT1
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 CEP44 Homo sapiens Q9C0F1
Y2H Pooling
16189514
Intra
RNF8 O76064 CEP44 Homo sapiens Q9C0F1
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 CEP44 Homo sapiens Q9C0F1
Validated Y2H
32296183
Intra
RNF8 O76064 CEP44 Homo sapiens Q9C0F1
Y2H Array
32296183
Intra
RNF8 O76064 CLK3 Homo sapiens P49761
Y2H Array
32296183
Intra
RNF8 O76064 CLK3 Homo sapiens P49761
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 CLK3 Homo sapiens P49761
Validated Y2H
32296183
Intra
RNF8 O76064 RBFOX2 Homo sapiens O43251
Validated Y2H
25416956
Intra
RNF8 O76064 RBFOX2 Homo sapiens O43251
Y2H Prey Pooling
25416956
Intra
RNF8 O76064 AMOTL2 Homo sapiens Q9Y2J4
Validated Y2H
32296183
Intra
RNF8 O76064 AMOTL2 Homo sapiens Q9Y2J4
Y2H Array
32296183
Intra
RNF8 O76064 AMOTL2 Homo sapiens Q9Y2J4
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 CALCOCO1 Homo sapiens Q9P1Z2
Y2H Array
32296183
Intra
RNF8 O76064 CALCOCO1 Homo sapiens Q9P1Z2
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 CALCOCO1 Homo sapiens Q9P1Z2
Validated Y2H
32296183
Intra
RNF8 O76064 CLK2 Homo sapiens P49760
Validated Y2H
32296183
Intra
RNF8 O76064 PICK1 Homo sapiens Q9NRD5
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 PICK1 Homo sapiens Q9NRD5
Validated Y2H
32296183
Intra
RNF8 O76064 PICK1 Homo sapiens Q9NRD5
Y2H Array
32296183
Intra
RNF8 O76064 TMEM79 Homo sapiens Q9BSE2
Y2H Prey Pooling
32296183
Intra
RNF8 O76064 TMEM79 Homo sapiens Q9BSE2
Validated Y2H
32296183
Intra
RNF8 O76064 TMEM79 Homo sapiens Q9BSE2
Y2H Array
25416956
Intra
RNF8 O76064 TMEM79 Homo sapiens Q9BSE2
Y2H Array
32296183
Intra
RNF8 O76064 TMEM79 Homo sapiens Q9BSE2
Y2H Array
31515488
Intra
RNF8 O76064 TMEM79 Homo sapiens Q9BSE2
Y2H Prey Pooling
25416956
Intra
RNF8 O76064 MAPK1 Homo sapiens P28482
Validated Y2H
32296183
Cross: Cross-species interaction Intra: Intraspecies interaction

RNF8 Antibodies

Cat. No. Product Name Application Reactivity
HY-P81599 RNF8 Antibody (YA1344) WB Human, Rat

Related Diseases

Diseases Alias
Riddle Syndrome

RIDL

Radiosensitivity-Immunodeficiency-Dysmorphic Features-Learning Difficulties Syndrome

Rnf168 Deficiency

Radiosensitivity, Immunodeficiency, Dysmorphic Facial Features, And Learning Difficulties

Radiosensitivity, Immunodeficiency, Dysmorphic Features, And Learning Difficulties
Dyskeratosis Congenita, Autosomal Recessive 3

DKCB3

Autosomal Recessive Dyskeratosis Congenita 3

Dyskeratosis Congenita, Autosomal Recessive, 3

Dyskeratosis Congenita, Autosomal Recessive, Type 3
Cornelia De Lange Syndrome

De Lange Syndrome

Brachmann De Lange Syndrome

Brachmann-De Lange Syndrome

Cdls

Bdls

Typus Degenerativus Amstelodamensis
Ataxia-Telangiectasia

Ataxia Telangiectasia

Louis-Bar Syndrome

AT

At1

Ataxia-Telangiectasia Syndrome

Ataxia - Telangiectasia Variant

Boder-Sedgwick Syndrome

Louis Bar Syndrome

Cerebello-Oculocutaneous Telangiectasia

Immunodeficiency With Ataxia Telangiectasia

A-T

Ataxia Telangiectasia Syndrome

Atm

Telangiectasia, Cerebello-Oculocutaneous

Ataxia-Telangiectasia Variant
Angelman Syndrome

AS

Happy Puppet Syndrome

Happy Puppet Syndrome, Formerly

Puppetlike Syndrome
Xeroderma Pigmentosum, Variant Type

Xeroderma Pigmentosum

XPV

Xeroderma Pigmentosum Variant Type

Xeroderma Pigmentosum With Normal Dna Repair Rates

Photosensitivity With Defective Dna Synthesis

Xp

De Sanctis-Cacchione Syndrome

Desanctis-Cacchione Syndrome

Xeroderma Pigmentosa

Xerodermic Idiocy

Xeroderma Pigmentosum Variant

Xp - [Xeroderma Pigmentosum]

Atrophoderma Pigmentosum
Fanconi Anemia, Complementation Group A

Fanconi Anemia

Fanconi Pancytopenia

Fanconi Anemia Complementation Group A

FANCA

Fa

Fanconi Panmyelopathy

Fanconi'S Anemia

Fanconi Anaemia

Fanconi'S Anaemia

Fanconi Hypoplastic Anemia

Estren-Dameshek Variant Of Fanconi Anemia

Estren-Dameshek Variant Of Fanconi Pancytopenia

Fanconi Anemia Estren-Dameshek Variant

Fanconis Anemia
Hereditary Breast Ovarian Cancer Syndrome

Hereditary Breast And Ovarian Cancer Syndrome

Brca1- And Brca2-Associated Hereditary Breast And Ovarian Cancer

Breast And/Or Ovarian Cancer

Breast And Ovarian Cancer Syndrome

Hboc Syndrome

Hereditary Breast And Ovarian Cancer

Brca1- Brca2-Associated Hboc
Diseases Alias
Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Macroglobulinemia Of Waldenstrom

Lymphoplasmacytic Lymphoma With Igm Gammopathy

Lymphoplasmacytic Lymphoma

Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Macroglobulinemia Of Waldenstrom

Lymphoplasmacytic Lymphoma With Igm Gammopathy

Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase
Bioactive Molecules (1)
Cat. No. Product Name Effect
Purity Rare Diseases
HY-RS12097 RNF8 Human Pre-designed siRNA Set A Pre-designed Sets / Unkown

Orthologs Information

Species Symbol Source ID
Rattus norvegicus RNF8 RGD RGD:1308035
Mus musculus RNF8 MGD MGI:1929069
Felis catus RNF8 VGNC VGNC:102507
Macaca mulatta RNF8 VGNC VGNC:76708
Canis familiaris RNF8 VGNC VGNC:45676
Bos taurus RNF8 VGNC VGNC:34073
Others RNF8 NCBI