2. Apoptosis Epigenetics Metabolic Enzyme/Protease MAPK/ERK Pathway PI3K/Akt/mTOR Immunology/Inflammation Stem Cell/Wnt
  3. Apoptosis Histone Methyltransferase Cytochrome P450 Caspase Survivin IAP p38 MAPK PI3K NO Synthase COX JNK ERK Akt
  4. Flavokawain A

Flavokawain A is a chalcone compound and an orally active inhibitor of PRMT5 and cytochrome P450. Flavokawain A has anti-inflammatory, anti-tumor, and immunomodulatory effects. Flavokawain A can inhibit the proliferation of tumor cells and induce apoptosis. Flavokawain A can be used in the research of diseases such as bladder cancer.

For research use only. We do not sell to patients.

Flavokawain A Chemical Structure

Flavokawain A Chemical Structure

CAS No. : 3420-72-2

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 52 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
50 mg USD 50 In-stock
100 mg USD 80 In-stock
200 mg   Get quote  
500 mg   Get quote  

Get it by May 19 for select sizes. Order within 17 hrs 55 mins.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Flavokawain A:

Top Publications Citing Use of Products

View All Histone Methyltransferase Isoform Specific Products:

View All Isoforms
EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D ASH1L/KMT2H SETDB1/KMT2G SETD2/KMT3A NSD2/MMSET/WHSC1 SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

View All Cytochrome P450 Isoform Specific Products:

View All Isoforms
CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All IAP Isoform Specific Products:

View All Isoforms
cIAP cIAP-1 cIAP-2 XIAP

View All p38 MAPK Isoform Specific Products:

View All Isoforms
p38 MAPK p38α p38β MAPK13 p38δ p38γ

View All PI3K Isoform Specific Products:

View All Isoforms
PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

View All NO Synthase Isoform Specific Products:

View All Isoforms
nNOS iNOS eNOS

View All COX Isoform Specific Products:

View All Isoforms
COX COX-1 COX-2 COX-3

View All JNK Isoform Specific Products:

View All Isoforms
JNK1 JNK2 JNK3 JNK

View All ERK Isoform Specific Products:

View All Isoforms
ERK ERK1 ERK2 ERK5 ERK8

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Flavokawain A is a chalcone compound and an orally active inhibitor of PRMT5 and cytochrome P450. Flavokawain A has anti-inflammatory, anti-tumor, and immunomodulatory effects. Flavokawain A can inhibit the proliferation of tumor cells and induce apoptosis. Flavokawain A can be used in the research of diseases such as bladder cancer[1][2][3][4][5].

In Vitro

Flavokawain A (0.05-25 μg/mL; 4-48 h) can inhibit the proliferation of multiple bladder cancer cells, cause the loss of mitochondrial membrane potential and the release of cytochrome c in T24 cells. The mechanism involves the activation of Bax and the inhibition of Bcl-x/L[1].
Flavokawain A (5-30 μg/mL; 4-24 h) induces apoptosis in T24 cells through the caspase pathway and by inhibiting XIAP and survivin[1].
Flavokawain A (20-40 μM; 24 h) can inhibit IL-1β-induced inflammation, the expression of senescence-related genes, apoptosis, and the phosphorylation of genes related to the MAPK and PI3K pathways in mouse chondrocytes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: RT4, T24, and EJ cells
Concentration: RT4 cells (0.05, 0.5, 5, 12.5 and 25 μg/mL)
T24 and EJ cells (0.309, 0.625, 1.25, 2.5, 5, 12.5 and 25 μg/mL)
Incubation Time: 48 h
Result: Inhibited cell proliferation in RT4, T24, and EJ bladder cancer cell lines

Western Blot Analysis[1]

Cell Line: T24 cells
Concentration: 12.5 μg/mL
Incubation Time: 4, 8, 16 and 24 h
Result: Inhibited the level Bcl-x/L.
Increased the level Bax.

Western Blot Analysis[1]

Cell Line: T24 cells
Concentration: 5, 10, 20 and 30μg/mL
Incubation Time: 4, 8, 16 and 24 h
Result: Inhibited the levels of XIAP and survivin.

Western Blot Analysis[2]

Cell Line: IL-1β treated mouse chondrocyte cells
Concentration: 20 and 40 μM
Incubation Time: 24 h
Result: Inhibited the levels of iNOS and COX2.
Inhibited the levels of P16 and P21.
Inhibited the level of Bax and increased the level of Bcl2.
Inhibited the phosphorylation levels of JNK, P38, ERK, PI3K, AKT and mTOR.
In Vivo

Flavokawain A (30 mg/kg; peritumoral injection; once every 3 days; 24 days) exhibits anti-tumor activity in a mouse model of bladder cancer[3].
Flavokawain A (50 mg/kg; oral administration; 28 days) shows no toxicity and has certain immunomodulatory effects in Balb/c mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male BALB/c mice aged 8 weeks old[4]
Dosage: 50 mg/kg/day
Administration: Oral administration (p.o.); 28 days
Result: Did not cause the death of mice during the 28-day treatment period, and there were no significant changes in the body weight of the mice.
Did not significantly alter several serum biochemical parameters.
Stimulated the proliferation of splenocytes, promoted the secretion of cytokines IL-2 and TNF-α, and increased the population of T cell subsets.
Decreased the levels of TNF-α and NO, and slightly reduced the level of IL-2.
Animal Model: Four-week-old Balb/c nude mice treated UMUC3 cells[3]
Dosage: 30 mg/kg
Administration: Peritumoral injection; once every 3 days; 24 days
Result: Significantly inhibited tumor size.
Decreased the arginine methylation level of histone and the expression of bladder-cancer-associated regulon genes.
Molecular Weight

314.33

Formula

C18H18O5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(OC)C=C(OC)C=C1O)/C=C/C2=CC=C(OC)C=C2
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (318.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1814 mL 15.9069 mL 31.8137 mL
5 mM 0.6363 mL 3.1814 mL 6.3627 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.93%

Data Sheet (279 KB) SDS (393 KB)

COA (246 KB) HNMR (165 KB) LCMS (113 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1814 mL 15.9069 mL 31.8137 mL 79.5343 mL
5 mM 0.6363 mL 3.1814 mL 6.3627 mL 15.9069 mL
10 mM 0.3181 mL 1.5907 mL 3.1814 mL 7.9534 mL
15 mM 0.2121 mL 1.0605 mL 2.1209 mL 5.3023 mL
20 mM 0.1591 mL 0.7953 mL 1.5907 mL 3.9767 mL
25 mM 0.1273 mL 0.6363 mL 1.2725 mL 3.1814 mL
30 mM 0.1060 mL 0.5302 mL 1.0605 mL 2.6511 mL
40 mM 0.0795 mL 0.3977 mL 0.7953 mL 1.9884 mL
50 mM 0.0636 mL 0.3181 mL 0.6363 mL 1.5907 mL
60 mM 0.0530 mL 0.2651 mL 0.5302 mL 1.3256 mL
80 mM 0.0398 mL 0.1988 mL 0.3977 mL 0.9942 mL
100 mM 0.0318 mL 0.1591 mL 0.3181 mL 0.7953 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Flavokawain A Related Classifications

Keywords:

Flavokawain A3420-72-2ApoptosisHistone MethyltransferaseCytochrome P450CaspaseSurvivinIAPp38 MAPKPI3KNO SynthaseCOXJNKERKAktCYPsPhosphoinositide 3-kinaseNitric oxide synthasesNOSCyclooxygenasec-Jun N-terminal kinaseExtracellular signal regulated kinasesPKBProtein kinase BEJ cellsanticarcinogenicchalconeT24Apoptotictumorbladder cancermouse chondrocytesInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Flavokawain A
Cat. No.:
HY-N2420
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.