Sodium hippurate, 98%  (Synonyms: N-Benzoylglycine sodium, 98%)

Sodium hippurate, 98% is an orally active metabolite. Sodium hippurate, 98% can be produced by intestinal microorganisms from the metabolism of polyphenols, benzoic acid. Sodium hippurate, 98% decreases NRF2, MMP9 and leads to ROS accumulation. Sodium hippurate, 98% activates TGFβ/SMAD signaling. Sodium hippurate, 98% improves hyperuricemia and colitis. Sodium hippurate, 98% can also be used in cardiovascular disease research^[1][2][3][4]. ^{[5][6][7][8][9]}.

Sodium hippurate, 98% (Hippuric acid, 25 μM; 24 h) co-incubated with Dexamethasone (HY-14648) significantly increases C2C12 myotube diameter, abrogates dexamethasone-induced reductions in myotube protein synthesis rates, and partially attenuates the increase in proteolysis in C2C12 myotubes under atrophy-like conditions^[2].
Sodium hippurate, 98% (Hippuric acid, 1-2 mM; 15 min) significantly reduces P-selectin/CD62P expression and inhibits PAC-1 activation-dependant antibody expression in platelets stimulated ex vivo via the P₂Y₁/P₂Y₁₂-adenosine diphosphate (ADP) pathway^[3].
Sodium hippurate, 98% (Hippuric acid, 0-1000 μM; 24 h) increases fibrosis-related gene expression, extracellular matrix imbalance, and oxidative stress in HK-2 cells^[4].
Sodium hippurate, 98% (Hippuric acid, 200-400 ng/mL; 24 h) increases ABCG2 expression and apical localization in Caco-2 cells^[5].
Sodium hippurate, 98% (Hippuric acid, 8 mg/mL; 24 h) decreases pro-inflammatory cytokine production and promotes the expression of ZO-1 in NCM460 cells treated with dextran sulfate sodium^[6].
Sodium hippurate, 98% (Hippuric acid, concentration equivalent to that in 2.5% serum from blueberry-diet-fed rats) inhibits osteoclastogenesis and osteoclast resorptive activity in nonadherent mouse bone marrow cells isolated from 4-week-old C57BL6/J mice^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sodium hippurate, 98% (Hippuric acid, 100 mg/kg; i.p.; five times per week; 10 weeks) promotes renal fibrosis and dysfunction in 5/6 nephrectomized rats^[4].
Sodium hippurate, 98% (Hippuric acid, 10 mg/kg; p.o.; daily; 4 weeks) alleviates hyperuricemia in mice by promoting intestinal urate excretion via enhancing ABCG2-mediated transport^[5].
Sodium hippurate, 98% (Hippuric acid, 50-150 mg/kg; p.o.; once daily; 7 days) alleviates DSS-induced colitis in male C57BL/6J mice, as shown by reduced clinical activity, improved intestinal barrier integrity, and modulated gut microbiota^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

201.16

C₉H₈NNaO₃

532-94-5

Solid

White to off-white

O=C(CNC(C1=CC=CC=C1)=O)O[Na]

Room temperature in continental US; may vary elsewhere.

Store at room temperature, keep dry and cool

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

• [1]. Bergmeyer H U, et al. Biochemical reagents[M]//Methods of Enzymatic Analysis. Academic Press, 1965: 967-1037.

  [2]. Edwards SJ, et al. (-)-Epicatechin and its colonic metabolite hippuric acid protect against dexamethasone-induced atrophy in skeletal muscle cells. J Nutr Biochem. 2022 Dec;110:109150.  [Content Brief]

  [3]. Santhakumar AB, et al. The ex vivo antiplatelet activation potential of fruit phenolic metabolite hippuric acid. Food Funct. 2015 Aug;6(8):2679-83.  [Content Brief]

  [4]. Sun B, et al. Hippuric Acid Promotes Renal Fibrosis by Disrupting Redox Homeostasis via Facilitation of NRF2-KEAP1-CUL3 Interactions in Chronic Kidney Disease. Antioxidants (Basel). 2020 Aug 25;9(9):783.  [Content Brief]

  [5]. Xu YX, et al. Alistipes indistinctus-derived hippuric acid promotes intestinal urate excretion to alleviate hyperuricemia. Cell Host Microbe. 2024 Mar 13;32(3):366-381.e9.  [Content Brief]

  [6]. Yang Y, et al. Hippuric acid alleviates dextran sulfate sodium-induced colitis via suppressing inflammatory activity and modulating gut microbiota. Biochem Biophys Res Commun. 2024 May 28;710:149879.  [Content Brief]

  [7]. Zhao H, et al. Hippuric acid and 3-(3-hydroxyphenyl) propionic acid inhibit murine osteoclastogenesis through RANKL-RANK independent pathway. J Cell Physiol. 2020 Jan;235(1):599-610.  [Content Brief]

  [8]. Griffith W H. Inhibition of tissue respiration by sodium benzoate and sodium hippurate. Proceedings of the Society for Experimental Biology and Medicine, 1937, 37(2): 279-280.

  [9]. Chen JR, et al. GPR109A mediates the effects of hippuric acid on regulating osteoclastogenesis and bone resorption in mice. Commun Biol. 2021 Jan 8;4(1):53.  [Content Brief]