2. Apoptosis NF-κB Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. Apoptosis NF-κB Topoisomerase Mitochondrial Metabolism Caspase
  4. 2-Methoxycinnamaldehyde

2-Methoxycinnamaldehyde  (Synonyms: o-Methoxycinnamaldehyde)

Cat. No.: HY-W046353 Purity: ≥98.0%
Data Sheet
SDS
COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

2-Methoxycinnamaldehyde (o-Methoxycinnamaldehyde) is a natural compound that can be isolated from Cinnamomum cassia. 2-Methoxycinnamaldehyde inhibits topoisomerase-I/II and NF-κB signaling pathway, causes mitochondrial dysfunction, induces lysosomal vesiculation, thereby leading to DNA damage and cell apoptosis. 2-Methoxycinnamaldehyde exhibits antitumor effects.

For research use only. We do not sell to patients.

2-Methoxycinnamaldehyde Chemical Structure

2-Methoxycinnamaldehyde Chemical Structure

CAS No. : 1504-74-1

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Based on 1 publication(s) in Google Scholar

Other Forms of 2-Methoxycinnamaldehyde:

2-Methoxycinnamaldehyde Related Antibodies

Top Publications Citing Use of Products

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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Topoisomerase Topo I Topo II

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

2-Methoxycinnamaldehyde (o-Methoxycinnamaldehyde) is a natural compound that can be isolated from Cinnamomum cassia. 2-Methoxycinnamaldehyde inhibits topoisomerase-I/II and NF-κB signaling pathway, causes mitochondrial dysfunction, induces lysosomal vesiculation, thereby leading to DNA damage and cell apoptosis. 2-Methoxycinnamaldehyde exhibits antitumor effects[1][2][3].

In Vitro

2-Methoxycinnamaldehyde (10-160 μM, 48 h) promotes the lactate dehydrogenase (LDH) release in cell NCI-H520, exhibits cytotoxicity, and inhibits the proliferation of NCI-H520 with an IC50 of 12.11 μM[2].
2-Methoxycinnamaldehyde (10-40 μM, 48 h) decreases mitochondrial membrane potential, activates caspase-3/9, induces apoptosis in NCI-H520[2].
2-Methoxycinnamaldehyde (0-50 μM, 48 h) modulates expressions of cell cycle regulatory proteins, inhibits TNF-α-induced MMP-9 expression, and inhibits the migration of human aortic smooth muscle cells (HASMCs)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-Methoxycinnamaldehyde Related Antibodies

Cell Viability Assay[2]

Cell Line: NCI-H520
Concentration: 10-160 μM
Incubation Time: 48 h
Result: Inhibited cell survival.

Apoptosis Analysis[2]

Cell Line: NCI-H520
Concentration: 10-40 μM
Incubation Time: 48 h
Result: Induced nuclear condensation, fragmentation, cell membrane blebbing, and apoptotic body formation.

Western Blot Analysis[3]

Cell Line: HASMC
Concentration: 0-50 μM
Incubation Time: 24 h
Result: Reduced the levels of cyclin D1, cyclin D3, and CDK6. Increased the levels of p21 and p27.
In Vivo

2-Methoxycinnamaldehyde (5-20 mg/kg, intratumoral injection, once daily for 49 days) inhibits the tumor growth and induces cell apoptosis of tumor cells in mouse NCI-H520 xenograft models[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mouse NCI-H520 xenograft models[3]
Dosage: 5-20 mg/kg
Administration: intratumoral injection, once daily for 49 days
Result: Inhibited tumor growth.
Molecular Weight

162.19

Formula

C10H10O2
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C/C=C/C1=CC=CC=C1OC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (616.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.1657 mL 30.8284 mL 61.6568 mL
5 mM 1.2331 mL 6.1657 mL 12.3314 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 98.95%

Data Sheet (278 KB) SDS (393 KB)

COA (249 KB) HNMR (122 KB) GC (213 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.1657 mL 30.8284 mL 61.6568 mL 154.1421 mL
5 mM 1.2331 mL 6.1657 mL 12.3314 mL 30.8284 mL
10 mM 0.6166 mL 3.0828 mL 6.1657 mL 15.4142 mL
15 mM 0.4110 mL 2.0552 mL 4.1105 mL 10.2761 mL
20 mM 0.3083 mL 1.5414 mL 3.0828 mL 7.7071 mL
25 mM 0.2466 mL 1.2331 mL 2.4663 mL 6.1657 mL
30 mM 0.2055 mL 1.0276 mL 2.0552 mL 5.1381 mL
40 mM 0.1541 mL 0.7707 mL 1.5414 mL 3.8536 mL
50 mM 0.1233 mL 0.6166 mL 1.2331 mL 3.0828 mL
60 mM 0.1028 mL 0.5138 mL 1.0276 mL 2.5690 mL
80 mM 0.0771 mL 0.3854 mL 0.7707 mL 1.9268 mL
100 mM 0.0617 mL 0.3083 mL 0.6166 mL 1.5414 mL
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2-Methoxycinnamaldehyde Related Classifications

Keywords:

2-Methoxycinnamaldehyde1504-74-1o-MethoxycinnamaldehydeApoptosisNF-κBTopoisomeraseMitochondrial MetabolismCaspaseNuclear factor-κBNuclear factor-kappaBNCI-H520HASMCantitumorInhibitorinhibitorinhibit

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