2. Metabolic Enzyme/Protease Neuronal Signaling Autophagy
  3. FAAH Autophagy
  4. JNJ-42165279

JNJ-42165279 is an orally active FAAH inhibitor, with IC50 values of 70 nM for hFAAH and 313 nM for rFAAH. JNJ-42165279 can be used in research related to the field of neuropathic pain.

For research use only. We do not sell to patients.

JNJ-42165279 Chemical Structure

JNJ-42165279 Chemical Structure

CAS No. : 1346528-50-4

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of JNJ-42165279:

JNJ-42165279 Related Antibodies

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  • Purity & Documentation

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Description

JNJ-42165279 is an orally active FAAH inhibitor, with IC50 values of 70 nM for hFAAH and 313 nM for rFAAH. JNJ-42165279 can be used in research related to the field of neuropathic pain[1][2].

In Vitro

JNJ-42165279 (10 μM, 1 hour) exhibited high selectivity and inhibitory activity against human and rat FAAH enzymes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JNJ-42165279 Related Antibodies
In Vivo

JNJ-42165279 (60 mg/kg, p.o., single dose) effectively alleviated neuropathic pain in the spinal nerve ligation (SNL)-induced neuropathic pain rat model[2].
High-dose JNJ-42165279 (100 mg/kg, p.o., once daily for 11 days) induced degenerative lens changes and cataracts in the embryo-fetal development process in Sprague-Dawley rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Spinal nerve ligation (SNL)-induced chronic tactile allodynia in Sprague-Dawley rats[2]
Dosage: 60 mg/kg
Administration: Oral gavage (p.o.), single dose
Result: Reversed tactile allodynia, with a maximum pain relief of 59% observed at 30 minutes, maintained for at least 2 hours.
Animal Model: Embryo-fetal development model in Sprague-Dawley rats[2]
Dosage: 10, 30, 100 mg/kg
Administration: Oral gavage (p.o.), once daily for 11 days
Result: induced fetal lens fiber hypertrophy and degeneration, with nuclear cataracts observed in some rats (100 mg/kg).
Clinical Trial
Molecular Weight

410.80

Formula

C18H17ClF2N4O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCN(CC2=CC=C(OC(F)(F)O3)C3=C2)CC1)NC4=C(Cl)C=CN=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (243.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4343 mL 12.1714 mL 24.3427 mL
5 mM 0.4869 mL 2.4343 mL 4.8685 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3 mg/mL (7.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3 mg/mL (7.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4343 mL 12.1714 mL 24.3427 mL 60.8569 mL
5 mM 0.4869 mL 2.4343 mL 4.8685 mL 12.1714 mL
10 mM 0.2434 mL 1.2171 mL 2.4343 mL 6.0857 mL
15 mM 0.1623 mL 0.8114 mL 1.6228 mL 4.0571 mL
20 mM 0.1217 mL 0.6086 mL 1.2171 mL 3.0428 mL
25 mM 0.0974 mL 0.4869 mL 0.9737 mL 2.4343 mL
30 mM 0.0811 mL 0.4057 mL 0.8114 mL 2.0286 mL
40 mM 0.0609 mL 0.3043 mL 0.6086 mL 1.5214 mL
50 mM 0.0487 mL 0.2434 mL 0.4869 mL 1.2171 mL
60 mM 0.0406 mL 0.2029 mL 0.4057 mL 1.0143 mL
80 mM 0.0304 mL 0.1521 mL 0.3043 mL 0.7607 mL
100 mM 0.0243 mL 0.1217 mL 0.2434 mL 0.6086 mL
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JNJ-42165279 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JNJ-421652791346528-50-4JNJ42165279JNJ 42165279FAAHAutophagyFatty acid amide hydrolaseEmbryo-fetal development modelSprague-Dawley ratsNeuropathic painInhibitorinhibitorinhibit

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