(R,S)-4-Hydroxy Cyclophosphamide-d₄ Preparation Kit

Recently, significant advancements in tyrosine-targeting electrophiles have primarily occurred in the field of protein-protein interactions (PPIs), where cysteine residues are often underrepresented and novel chemistries are needed to address these interfaces. In this context, tyrosines are frequently more accessible compared to more buried binding sites. Moreover, they are commonly found at "hot spots," which are functional epitopes of PPIs, with 12.3% of the residues consisting of tyrosines. This prevalence is likely due to the hydrophobic nature of tyrosine, its ability to participate in aromatic π-interactions, and its capacity for hydrogen bonding. Beyond PPIs, some progress has also been made in covalent tyrosine targeting in other areas where more commonly addressed side chains are lacking. Even though tyrosine has a slightly lower pKa value compared to the protonated lysine side chain (approximately 10 vs. 10.5 for the unprotected amino acid side chains), significantly less progress has been made in the development of tyrosine-targeted covalent ligands compared to lysine. This is likely due to the reduced flexibility of the tyrosine side chain and the greater steric hindrance of its hydroxy group, which makes it more challenging to adopt suitable reaction geometries.

Through careful selection, we constructed a structural filter containing over 110 electrophilic groups. By analyzing the electrophilic fragments selected by the structural filter, we removed any molecules with trivial or undesirable structural features. Ultimately, we obtained 124 fragment molecules which can target tyrosine residue and can be used for fragment-based covalent drug discovery.

MCE ATP Assay Kit provides a rapid method to measure intracellular ATP. The kit is suitable for ATP determination in cells, tissue, and other biological samples.

MCE Cytotoxicity LDH Assay Kit can detect LDH activity in the culture supernatant, which is indicative of cytotoxicity. Meanwhile viable cell number can be detected by CCK8 Kit (HY-K0301).

MCE One Step TUNEL Apoptosis Detection Kit (FITC) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show green fluorescence.

MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show red fluorescence.

MCE Apoptosis and Necrosis Assay Kit provides a rapid and convenient method to detect cell apoptosis and necrosis.

MCE Cell Apoptosis Analysis Kit (Hoechst staining) provides a rapid and convenient method to detect cell apoptosis.

MCE Cell Senescence β-Galactosidase Staining Kit can be used to evaluate the senescence of cells or tissues.

MCE Seamless DNA Assembly Ultra Kit is a next-generation recombinant cloning kit that allows the recombination of single or multiple DNA fragments in a single reaction.

MCE ROS Assay Kit utilizes the fluorescent probe DCFH-DA to detect ROS.

MCE Antibody Labeling Kit-VF 488 can efficiently and covalently attach the VF 488 dye to antibodies.

MCE Nitric Oxide Detection Kit employs a modified Griess method to detect the content of Nitric Oxide in various samples.

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.

MCE Mitochondria Isolation Kit for Tissue enables the fast and efficient isolation of intact mitochondria from tissue using differential centrifugation.

MCE TUNEL Apoptosis Detection Kit (Biotin) can be used to detect apoptosis in cultured cells, as well as in frozen or paraffin sections.

MCE VF 647A-Annexin V/PI Apoptosis Detection Kit can be utilized to detect the level of cell apoptosis.

MCE Bradford Protein Assay Kit is suitable for detecting the total protein content in samples such as serum, plasma, animal tissues, and cells.

MCE Fumarase Activity Assay Kit (WST-8) can be used to measure the amount of fumarase present in biological samples.

MCE Mitochondria Isolation Kit for Cultured Cells enables the fast and efficient isolation of intact mitochondria from cells using differential centrifugation.

MCE Caspase 1 Activity Assay Kit provides a simple and convenient way for detecting the activity of detection of Caspase 1 activity that based on the spectrophotometric.

MCE Caspase 3 Activity Assay Kit provides a simple and convenient way for detecting the activity of detection of Caspase 3 activity that based on the spectrophotometric.

MCE Lipid Peroxidation (MDA) Assay Kit is suitable for measuring MDA levels in a variety of samples including plasma, serum, urine, tissues or cell lysates.

MCE Ultra High Sensitivity ECL Kit is an ultra-sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) labeled antibodies on western blots.

MCE Cell-ATP Viability Detection Kit is used for detecting the number and viability of living cells in culture based on high-sensitivity bioluminescence detection technology of the ATP present.

MCE VF 488 Caspase 3 Assay Kit for Live Cells uses a fluorescent substrate with cell membrane permeability to detect Caspase 3 activity in live cells.

MCE GSH/GSSG Assay Kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, and tissue and cell extracts.

MCE Human CD3⁺ T Cells Negative Selection Kit is designed for the isolation of CD3⁺ T cells from human peripheral blood mononuclear cells (PBMC).

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription. With advanced reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically.

MCE Renilla-Firefly Luciferase Dual Assay Kit is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture.

MCE Mouse CD3⁺ T Cells Negative Selection Kit is designed for the isolation of CD3⁺ T cells from single cell suspensions of mouse spleen cells and lymph nodes.

MCE Mouse CD4⁺ T Cells Negative Selection Kit is designed for the isolation of CD4⁺ T cells from single cell suspensions of mouse spleen cells and lymph nodes.

MCE Mouse CD8⁺ T Cells Negative Selection Kit is designed for the isolation of CD8⁺ T cells from single cell suspensions of mouse spleen cells and lymph nodes.

MCE Mouse CD8⁺ Cells Positive Selection Kit is designed for the isolation of CD8⁺ cells from single cell suspensions of mouse spleen cells, lymph nodes or other tissues.

MCE NADP⁺/NADPH Assay Kit (WST-8) is a colorimetric assay that conveniently detects the oxidized (NADP⁺) and reduced (NADPH) forms of coenzyme Ⅱ within cells, tissues, and other samples.

MCE NAD⁺/NADH Assay Kit (WST-8) is a colorimetric assay that conveniently detects the oxidized (NAD⁺) and reduced (NADH) forms of coenzyme Ⅰ within cells, tissues, and other samples.

MCE JC-1 Mitochondrial Membrane Potential Assay Kit uses JC-1 to detect the mitochondrial membrane potential in variety of cell types, as well as intact tissues and isolated mitochondria.

MCE Mouse CD4⁺ T Cells Positive Selection Kit is designed for the isolation of CD4⁺ cells from single cell suspensions of mouse spleen cells, lymph nodes or other tissues.

MCE Hematoxylin-Eosin Staining Kit can be used for cell staining, the combination of hematoxylin and eosin results in a staining pattern where the cytoplasm appears red or pink and the nucleus takes on a blue or blue-violet.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from plasma and serum that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

Sensitive ECL Kit enables low picogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems.

MCE Human CD34⁺ Cells Negative Selection Kit is designed for the isolation of CD34⁺ cells from human cord blood mononuclear cells (CBMC) or human peripheral blood mononuclear cells (PBMC).

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cell culture media that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

High Sensitivity ECL Kit enables low picogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems.

Annexin V-mCherry Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

MCE Exosome Protein Detection Kit (CD63&TSG101) can specifically detect exosome proteins CD63 and TSG101. This product is suitable for the detection of human, rat and mouse exosomes.

MCE Human Cholangiocarcinoma Organoid Kit contains Cholangiocarcinoma Organoid Basal Medium A, Cholangiocarcinoma Organoid Supplement B (50×), Cholangiocarcinoma Organoid Supplement C (250×). This product can be used to efficiently construct human cholangiocarcinoma organoid.

MCE PBS-T Powder (1 L of 1×) consists of NaCl, KCl, Phosphate and Tween 20, and is widely used in ELISA and Western Blot, and can also be used for antibody dilution and preparation of blocking solution.

MCE TBS Powder (1 L of 1×) consists of NaCl, KCl and Tris, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

MCE Annexin V-PE Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription and can eliminate genomic DNA (gDNA) contaminations in RNA samples. With updated reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically.