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ATPase activity

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By Targets:	Na+/K+ ATPase (25) Akt (1) Antibiotic (9) Apoptosis (28) Aryl Hydrocarbon Receptor (1) ATP Synthase (4) Autophagy (24) Bacterial (24) BCRP (2) Biochemical Assay Reagents (1) Calcium Channel (2) CDK (2) CFTR (1) Cholinesterase (ChE) (1) DNA/RNA Synthesis (7) Dynamin (1) Endogenous Metabolite (1) Epigenetic Reader Domain (2) Estrogen Receptor/ERR (7) Fungal (12) GSK-3 (2) Hedgehog (1) HIF/HIF Prolyl-Hydroxylase (2) HIV (1) HSP (19) HSV (5) Influenza Virus (1) IRAK (2) Isotope-Labeled Compounds (4) Kinesin (7) LXR (1) Microtubule/Tubulin (3) Mitophagy (2) Myosin (6) NF-κB (3) NOD-like Receptor (NLR) (4) Oxidative Phosphorylation (3) P-glycoprotein (5) p97 (4) Parasite (7) Phosphatase (1) PKA (1) Potassium Channel (3) Prostaglandin Receptor (1) Proton Pump (33) RSV (1) SARS-CoV (1) SphK (1) TGF-beta/Smad (2) Thyroid Hormone Receptor (1) TOPK (2) Topoisomerase (1) ULK (1) VSV (1)
By Research Areas:	Cancer (90) Cardiovascular Disease (13) Endocrinology (7) Infection (34) Inflammation/Immunology (28) Metabolic Disease (5) Neurological Disease (15)
Click Chemistry:	Alkynes (1)

162

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides

Natural
Products

Isotope-Labeled Compounds

Click Chemistry

S-Trityl-L-cysteine NSC 83265; S-Tritylcysteine; 3-Tritylthio-L-alanine	Kinesin Apoptosis	Cancer
S-Trityl-L-cysteine (NSC 83265) is a selective and allosteric kinesin Eg5 inhibitor with an IC₅₀ of 1 μM for the inhibition of basal ATPase activity and 140 nM for the microtubule-activated ATPase activity. S-Trityl-L-cysteine has antitumor activities .

MAL3-101	HSP	Cancer
MAL3-101 is a potent HSP70 allosteric inhibitor. MAL3-101 inhibits HSP70 ATPase activity by blocking Hsp40 co-chaperone interaction. MAL3-101 can be used for researching muscle invasive bladder cancer (MIBC) .

CCT018159	HSP Apoptosis	Cancer
CCT018159, a 3,4-diaryl pyrazoleresorcinol, is a ATP-competitive HSP90 ATPase activity inhibitor with IC₅₀s of 3.2 and 6.6 µM for human Hsp90β and yeast Hsp90, respectively. CCT018159 caused cell cytostasis associated with a G1 arrest and induces apoptosis. CCT018159 inhibits key endothelial and tumor cell functions implicated in invasion and angiogenesis .

Displurigen NSC375009	HSP	Others
Displurigen (NSC375009) disrupts human embryonic stem cell pluripotency by targeting HSPA8. Displurigen inhibits ATPase activity of HSP70 (IC₅₀ = 225 μM) .

AZ99	BCRP	Cancer
AZ99 (compound 7) is a potent ABCG2 inhibitor. AZ99 inhibits ABCG2-mediated transport of estrone-3-sulfate (E1S) into proteoliposomes and ABCG2 ATPase activity .

G247	Bacterial	Infection
G247 is a specific MsbA inhibitor. G247 acts as a transmembrane domains (TMDs) wedge, symmetrically increasing nucleotide-binding domains (NBDs) separation and preventing conformational transition of MsbA. G247 suppresses ATPase activity by increasing inter-NBD distance .

BGC-20-1531 PGN 1531	Prostaglandin Receptor PKA	Others
BGC-20-1531 (PGN 1531), a benzenesulfonamide derivative, is a selective EP4 antagonist. BGC-20-1531 binds to PGE2 enhancing the ATPase activity, which are coupled to Gs proteins and thus activate adenylate cyclase generating cAMP and activating PKA. BGC-20-1531 is promising for research of liver disorders .

WS-917	P-glycoprotein	Cancer
WS-917 is a potent and orally active ABCB1 modulator. WS-917 enhances ABCB1 ATPase activity. WS-917 significantly enhances the antitumor effect of paclitaxel combined with anti-PD-1 antibody. WS-917 promotes CD8 ⁺ T-cell activation .

ATPase-IN-4	Others	Cancer
ATPase-IN-4 (Compound MS1) inhibits the ATPase activity of EHD4 (IC₅₀: 0.92 μM). ATPase-IN-4 also inhibits EHD2 enzymatic activity

Depramine GP 31406	Cholinesterase (ChE)	Neurological Disease
Depramine (GP 31406) is a tricyclic antidepressant with pharmacologically activity. Depramine inhibits acetylcholinesterase，Mg ²⁺-ATPase，and Na+/K+ ATPase activity .

ATPase-IN-2	Others	Others
ATPase-IN-2 is an *ATPase* inhibitor with an IC₅₀ value of 0.9 μM. ATPase-IN-2 inhibits C. difficile toxin B (TcdB) glycohydrolase activity with an AC₅₀ value of 30.91 μM. ATPase-IN-2 can be used for the research of ATP-related .

Tubulin polymerization/V-ATPase-IN-1	Microtubule/Tubulin Proton Pump Apoptosis	Inflammation/Immunology Cancer
Tubulin polymerization/V-ATPase-IN-1 (compound F10) is a Tubulin polymerization/V-ATPase inhibitor. Tubulin polymerization/V-ATPase-IN-1 shows robust antiproliferation activity against four human cancer cell lines, and exerts antiproliferative activity by inhibiting tubulin and V-ATPase. Tubulin polymerization/V-ATPase-IN-1 induces immunogenic cell death in addition to apoptosis, and inhibits tumor growth in an RM-1 homograft model with enhanced T lymphocyte infiltration .

Bafilomycin D	Proton Pump Bacterial Fungal	Infection
Bafilomycin D is a specific inhibitor of vacuolar-type ATPase (V-ATPase). Bafilomycin D has antimicrobial, insecticidal, herbicidal and cytotoxic activity .

Citreoviridin	Na+/K+ ATPase Apoptosis	Infection Cardiovascular Disease
Citreoviridin, a toxin from Penicillium citreoviride NRRL 2579, inhibits brain synaptosomal Na ⁺/K ⁺-ATPase whereas in microsomes, both Na ⁺/K ⁺-ATPase and Mg ²⁺-ATPase activities are significantly stimulated in a dose-dependent manner . Citreoviridin inhibits cell proliferation and enhances apoptosis of human umbilical vein endothelial cells .

V-ATPase-IN-1	Proton Pump	Others
V-ATPase-IN-1 (Compound 3b-03) is a Vacuolar-type H ⁺-ATPases (V-ATPase) inhibitor (IC₅₀ = 194.80 μM) that can effectively target the *V-ATPase* subunit A (K_d = 0.803 μM). V-ATPase-IN-1 exhibits insecticidal activity against M. separata (LC₅₀ = 2.64 mM) and contributes to research in the development of chemical insecticides .

NSC819701	p97 ATP Synthase	Cancer
NSC819701 is a triazole inhibitor of the p97 ATPase. NSC819701 inhibits the activity of p97 ATPase by binding to a specific site of p97 .

RUVBL1/2 ATPase-IN-1	Others	Cancer
RUVBL1/2 ATPase-IN-1 (compound 18) is a potent and selective inhibitor of RUVBL1/2 ATPase with IC₅₀ values of 6.0 and 7.7 μM, respectively. RUVBL1 and RUVBL2 are highly conserved AAA ATPases (ATPases Associated with various cellular Activities) and highly relevant to the progression of cancer. RUVBL1/2 ATPase-IN-1 has the potential for the research of cancer diseases .

Bassianin	Others	Others
Bassianin is a compound isolated from fungi that has the activity of inhibiting erythrocyte membrane ATPase and causing cell lysis. Its inhibitory effect on ATPase may be due to membrane disruption.

Oligomycin A 30+ Cited Publications MCH 32	ATP Synthase Fungal Antibiotic	Infection Cancer
Oligomycin A (MCH 32), created by Streptomyces, acts as a mitochondrial *F₀F₁-ATPase* inhibitor, with a K_i of 1 μM; Oligomycin A shows anti-fungal activity.

2-Acetylfuran-d3 2-Furyl methyl ketone-d₃	Isotope-Labeled Compounds	Others
2-Acetylfuran-d₃ is deuterated labeled Citreoviridin (HY-N6745). Citreoviridin, a toxin from Penicillium citreoviride NRRL 2579, inhibits brain synaptosomal Na ⁺/K ⁺-ATPase whereas in microsomes, both Na ⁺/K ⁺-ATPase and Mg ²⁺-ATPase activities are significantly stimulated in a dose-dependent manner . Citreoviridin inhibits cell proliferation and enhances apoptosis of human umbilical vein endothelial cells .

TBT1	Bacterial Antibiotic	Infection
TBT1 is a first-generation inhibitor of the MsbA transporter. TBT1 is an LPS transport inhibitor and MsbA ATPase stimulator in strains from the Acinetobacter genus. TBT1 stimulated ATPase activity with an EC₅₀ of 13 µM .

NSC799462	p97 ATP Synthase	Cancer
NSC799462 is a triazole inhibitor of p97 ATPase. NSC799462 inhibits p97 ATPase activity (IC₅₀ = 15 nM) by binding to a specific site on p97 and causing local changes in the p97 structure .

(-)-Blebbistatin 10+ Cited Publications	Myosin	Cardiovascular Disease Cancer
(-)-Blebbistatin is a selective inhibitor of the ATPase activity of non-muscle myosin II .

Oleandrin 3 Publications Verification PBI-05204	Na+/K+ ATPase	Cancer
Oleandrin (PBI-05204) inhibits the Na ⁺, K ⁺-ATPase activity with an IC₅₀ of 620 nM.

Ro 18-5364	Others	Others
Ro 18-5364 is an inhibitor of gastric (H? + K?)-ATPase, which has the effect of inhibiting the activity of the enzyme. Ro 18-5364 has a very strong inhibitory effect on gastric mucosal (H? + K?)-ATPase, and the inhibitory effect is more significant at low pH. At the same time, there is no selectivity difference between the two enantiomers of (H? + K?)-ATPase, and its inhibitory effect can be studied and verified by a variety of experimental methods, including inhibition of enzyme activity, proton transport, and studies on its binding.

Ciliobrevin D 3 Publications Verification	Hedgehog	Cancer
Ciliobrevin D is a cell-permeable, reversible and specific inhibitor of AAA+ ATPase motor cytoplasmic dynein. Ciliobrevin D inhibits Hedgehog (Hh) signaling and primary cilia formation. Ciliobrevin D inhibits dynein-dependent microtubule gliding and ATPase activity in vitro .

Strophanthidin	Na+/K+ ATPase	Cardiovascular Disease
Strophanthidin is a naturally available cardiac glycoside . Strophanthidin 0.1 and 1 nmol/L increases and 1~100 μmol/L inhibits the *Na+/K+-ATPase* activities, but Strophanthidin 10 and 100 nmol/L does not affect *Na+/K+-ATPase* activities in cardiac sarcolemmal . Strophanthidin increases both diastolic and systolic intracellular Ca ²⁺ concentration .

Oleandrigenin	Na+/K+ ATPase	Cardiovascular Disease Cancer
Oleandrigenin is a potent cardiotonic steroid. Oleandrigenin shows Na ⁺/K ⁺-ATP-ase inhibiting and cytotoxic activities .

Dynapyrazole-A	Dynamin	Infection
Dynapyrazole A is a specific inhibitor of microtubule dynamin that specifically inhibits the ATPase activity of microtubule-stimulated dynamin without blocking microtubule-independent basal activity .

Protonstatin-1	Proton Pump	Others
Protonstatin-1 is a selective plasma membrane (PM) H ⁺-ATPase inhibitor (IC₅₀ of 3.9 μM) that inhibits auxin transport. Protonstatin-1 interacts with the PM H ⁺-ATPase central loop and may thus impede the functions of the N- and/or P-domain to inhibit the pump activity .

TS 155-2 JBIR 100	Antibiotic Bacterial Proton Pump	Infection Inflammation/Immunology Endocrinology
TS 155-2 (JBIR 100), an antimicrobial agent, is a Bafilomycin analogue and an antibiotic. TS 155-2 can be isolated from a culture of Streptomyces sp. TS 155-2 inhibits *V-ATPase* activity. V-ATPase is correlated with an increasing number of diseases such as osteopetrosis, male infertility and renal acidosis .

Chloroprocaine hydrochloride 2-Chloroprocaine hydrochloride	Na+/K+ ATPase	Neurological Disease
Chloroprocaine hydrochloride (2-Chloroprocaine hydrochloride) is a potent inhibitor of *Na,K-ATPase* activity with an IC₅₀ of 13 mM. Chloroprocaine hydrochloride blocks peripheral nerve .

Chloroprocaine 2-Chloroprocaine	Na+/K+ ATPase	Neurological Disease
Chloroprocaine (2-Chloroprocaine) is a potent inhibitor of *Na,K-ATPase* activity with an IC₅₀ of 13 mM. Chloroprocaine blocks peripheral nerve .

HSP90-IN-25	HSP	Cancer
HSP90-IN-25 (compound 4a) is an HSP90 inhibitor that specifically inhibits the ATPase activity of HSP90 .

BTS 2 Publications Verification N-Benzyl-p-toluenesulfonamide; N-Tosylbenzylamine	Myosin	Others
BTS (N-Benzyl-p-toluenesulfonamide) is a potent and selective inhibitor of skeletal muscle myosin II subfragment 1 (S1) ATPase activity, with an IC₅₀s of ~5 μM for actin- and Ca ²⁺-stimulated myosin S1 ATPase. BTS specifically inhibits the contraction of fast skeletal muscle fibers .

HA15 25+ Cited Publications	HSP Autophagy Apoptosis	Cancer
HA15 is a potent and specific inhibitor of ER chaperone BiP/GRP78/HSPA5, inhibits the ATPase activity of BiP, with anti-cancerous activity .

DN-F01	Others	Others
DN-F01 has a strong inhibitory calcium-dependent effect on cardiac myofibrillar ATPase activity with an IC₅₀ value of 11 ± 4 nmol/L.

Strophanthidin (Standard)	Na+/K+ ATPase	Cardiovascular Disease
Strophanthidin (Standard) is the analytical standard of Strophanthidin. This product is intended for research and analytical applications. Strophanthidin is a naturally available cardiac glycoside . Strophanthidin 0.1 and 1 nmol/L increases and 1~100 μmol/L inhibits the Na+/K+-ATPase activities, but Strophanthidin 10 and 100 nmol/L does not affect Na+/K+-ATPase activities in cardiac sarcolemmal . Strophanthidin increases both diastolic and systolic intracellular Ca2+ concentration .

Chebulinic acid 2 Publications Verification	DNA/RNA Synthesis TGF-beta/Smad Proton Pump	Infection
Chebulinic acid is a potent natural inhibitor of M. tuberculosis DNA gyrase, also can inhibit SMAD-3 phosphorylation, inhibit H+ K+-ATPase activity.

Aurein 2.3	Antibiotic Bacterial	Infection
Aurein 2.3 is an antibiotic antimicrobial peptide. Aurein 2.3 partially inhibits E.coli ATPase activity and inhibits cell growth .

Licoflavone B	Parasite	Infection
Licoflavone B is a flavonoid isolated from Glycyrrhiza inflata, inhibits S. mansoni *ATPase* (IC₅₀, 23.78 µM) and ADPase (IC₅₀, 31.50 µM) activity. Anti-schistosomiasis activity .

Phenamacril JS399-19	Myosin Fungal	Infection
Phenamacril (JS399-19) inhibits activity of myosin I non-competitively with an IC₅₀ of 360 nM through suppression of the ATPase activity, exhibits therefore an antifungal efficacy towards Fusarium species .

Acevaltrate 2 Publications Verification	Na+/K+ ATPase	Neurological Disease
Acevaltrate inhibits the Na ⁺/K ⁺-ATPase activity in the rat kidney and brain hemispheres with IC₅₀s of 22.8 μM and 42.3 μM, respectively .

Oleandrin (Standard)	Na+/K+ ATPase	Cancer
Oleandrin (Standard) is the analytical standard of Oleandrin. This product is intended for research and analytical applications. Oleandrin (PBI-05204) inhibits the Na+, K+-ATPase activity with an IC50 of 620 nM.

CB-6644 10+ Cited Publications	Others	Cancer
CB-6644 is a selective inhibitor of RUVBL1/2 complex with anti-cancer activity. CB-6644 blocks the ATPase activity of RUVBL1/2 with an IC₅₀ of 15 nM .

SW02	HSP	Neurological Disease
SW02 is a potent activator of *ATPase* activity of Hsp70, with an EC₅₀ of 150 μM. SW02 leads to accumulation of both total tau and phosphorylated tau (pTau) .

Cat. No.	Product Name	Type

HY-D1435

Oxonol VI	Fluorescent Dyes/Probes
Oxonol VI acts as an optical indicator for membrane potentials in lipid vesicles. Oxonol VI is suitable for detecting changes of membrane potential associated with the activity of the (Na ⁺ + K ⁺)-ATPase in reconstituted vesicles .

HY-13441G

(-)-Blebbistatin (GMP)	Fluorescent Dye
(-)-Blebbistatin (GMP) is (-)-Blebbistatin (HY-13441) produced by using GMP guidelines. (-)-Blebbistatin (GMP) is a selective inhibitor of the ATPase activity of non-muscle myosin II .

HY-D2360

O-Carboranylphenoxyacetanilide	Fluorescent Dyes/Probes
O-Carboranylphenoxyacetanilide is a HIF-1α inhibitor and inhibits HIF-1α activation. O-Carboranylphenoxyacetanilide inhibits transcriptional activity of HIF in HeLa cells (IC₅₀: 0.74 μM). O-Carboranylphenoxyacetanilide inhibits HSP60 chaperone activity and HSP60 ATPase activity .

Cat. No.	Product Name	Type

HY-W019981C

Magnesium chloride hexahydrate, for cell culture MgCl2 hexahydrate, for cell culture	Buffer Reagents
Magnesium chloride hexahydrate, for cell culture is the hexahydrate form of magnesium dichloride. Magnesium chloride hexahydrate, for cell culture can be used as a source of magnesium ion and a co-foactor for many enzymes. Magnesium chloride hexahydrate, for cell culture increases the activity of Ca ²⁺ ATPase .

HY-13441G

(-)-Blebbistatin (GMP)	Biochemical Assay Reagents
(-)-Blebbistatin (GMP) is (-)-Blebbistatin (HY-13441) produced by using GMP guidelines. (-)-Blebbistatin (GMP) is a selective inhibitor of the ATPase activity of non-muscle myosin II .

HY-W583212

Zn(II) Mesoporphyrin IX

ZnMP

Cell Assay Reagents

Zn(II) Mesoporphyrin IX (ZnMP) is a specific activator of ABCB10, significantly increasing its ATPase activity and acting as a substrate for ABCB10 in heme synthesis. Zn(II) Mesoporphyrin IX may promote the transcription of hemoglobinization genes by facilitating the degradation of the Bach1 repressor. Zn(II) Mesoporphyrin IX aids in studying the potential roles of ABCB10 in heme synthesis, oxidative stress protection, hepatitis C, and other areas .

Cat. No.	Product Name	Target	Research Area

HY-P5965

BiP substrate	Peptides	Others
BiP substrate is a peptide substrate of BiP that can be used to measure the BiP ATPase activity .

HY-P2657

Verucopeptin	HIF/HIF Prolyl-Hydroxylase Proton Pump Endogenous Metabolite	Cancer
Verucopeptin is a potent HIF-1 (IC50=0.22 μM) inhibitor and decreases the expression of HIF-1 target genes and HIF-1α protein levels. Verucopeptin strongly inhibits v-ATPase activity by directly targeting the v-ATPase ATP6V1G subunit but not ATP1V1B2 or ATP6V1D. Verucopeptin exhibits antitumor activity against multidrug resistance (MDR) cancers and can be used for cancer research.

HY-125486

Reversin 121	P-glycoprotein	Cancer
Reversin 121 is a P-glycoprotein inhibitor. Reversin 121 increases the ATPase activity of MDR1. Reversin 121 reverses P-glycoprotein-mediated multidrug resistance. Reversin 121 can be used in the research of cancers .

HY-P5570

Aurein 2.3	Antibiotic Bacterial	Infection
Aurein 2.3 is an antibiotic antimicrobial peptide. Aurein 2.3 partially inhibits E.coli ATPase activity and inhibits cell growth .

Cat. No.	Product Name	Chemical Structure

HY-13757AS

Tamoxifen-d5
Tamoxifen-d₅ is a deuterium labeled Tamoxifen. Tamoxifen (ICI 47699) is an orally active, selective estrogen receptor modulator (SERM). Tamoxifen is a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity[1][2].

HY-13757S

Tamoxifen-d3 hydrochloride

Tamoxifen-d₃ hydrochloride is deuterated labeled Tamoxifen (Citrate) (HY-13757). Tamoxifen Citrate (ICI 46474) is an orally active, selective estrogen receptor modulator (SERM) which blocks estrogen action in breast cells and can activate estrogen activity in other cells, such as bone, liver, and uterine cells .Tamoxifen Citrate is a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen Citrate also potent inhibits infectious EBOV Zaire and Marburg (MARV) with IC₅₀ of 0.1 μM and 1.8 μM, respectively . Tamoxifen Citrate activates autophagy and induces apoptosis .Tamoxifen Citrate also can induce gene knockout of CreER(T2) transgenic mouse .

HY-W015912S

2-Acetylfuran-d3

2-Acetylfuran-d₃ is deuterated labeled Citreoviridin (HY-N6745). Citreoviridin, a toxin from Penicillium citreoviride NRRL 2579, inhibits brain synaptosomal Na ⁺/K ⁺-ATPase whereas in microsomes, both Na ⁺/K ⁺-ATPase and Mg ²⁺-ATPase activities are significantly stimulated in a dose-dependent manner . Citreoviridin inhibits cell proliferation and enhances apoptosis of human umbilical vein endothelial cells .

HY-17507S

Pantoprazole-d6

Pantoprazole-d₆ is deuterium labeled Pantoprazole. Pantoprazole (BY10232) is an orally active and potent proton pump inhibitor (PPI)[1]. Pantoprazole, a substituted benzimidazole, is a potent H+/K+-ATPase inhibitor with an IC50 of 6.8 μM. Pantoprazole improves pH stability and has anti-secretory, anti-ulcer activities. Pantoprazole significantly increased tumor growth delay combined with Doxorubicin (HY-15142)[3][4].

HY-17507S1

Pantoprazole-d3

Pantoprazole-d₃ is deuterium labeled Pantoprazole. Pantoprazole (BY10232) is an orally active and potent proton pump inhibitor (PPI)[1]. Pantoprazole, a substituted benzimidazole, is a potent H+/K+-ATPase inhibitor with an IC50 of 6.8 μM. Pantoprazole improves pH stability and has anti-secretory, anti-ulcer activities. Pantoprazole significantly increased tumor growth delay combined with Doxorubicin (HY-15142)[3][4].

HY-13757AS1

Tamoxifen-d3

Tamoxifen-d₃ is the deuterium labeled Tamoxifen[1]. Tamoxifen (ICI 47699) is an orally active, selective estrogen receptor modulator (SERM) which blocks estrogen action in breast cells and can activate estrogen activity in other cells, such as bone, liver, and uterine cells[2][3][4]. Tamoxifen is a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen also potent inhibits infectious EBOV Zaire and Marburg (MARV) with IC50 of 0.1 μM and 1.8 μM, respectively[6]. Tamoxifen activates autophagy and induces apoptosis[5]. Tamoxifen also can induce gene knockout of CreER(T2) transgenic mouse[7].

Cat. No.	Product Name		Classification

HY-16062

ARQ 621		Alkynes
ARQ 621 is an allosteric, potent and selective inhibitor of Eg5, a microtubule-based ATPase motor protein involved in cell division. Anti-tumor activity . ARQ 621 is a kinesin inhibitor . ARQ 621 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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