culture

Exosomes are small membrane vesicles of endocytic origin that are secreted by most cells in culture. Exosomes contain nucleic acids, proteins, lipids, amino acids, and metabolites, etc. Their diverse constituents can reflect their cell of origin. Exosomes are associated with immune responses, viral pathogenicity, pregnancy, cardiovascular diseases, central nervous system-related diseases, and cancer progression. Proteins, metabolites, and nucleic acids delivered by exosomes into recipient cells effectively alter their biological response. Such exosome-mediated responses can be disease promoting or restraining.

The biology of exosomes in disease is still emerging, and the number of studies addressing their utility in the diagnosis and treatment of various pathologies has increased substantially. MCE supplies a unique collection of 45 compounds with the activity of inhibiting or stimulating exsomes secretion/biosynthesis. MCE Exosomes Compound Library is a useful tool for exsomes research.

MCE Basement Membrane Matrix for Organoid Culture is primarily composed of natural basement membrane matrix extracted from mouse tumors. This product is mainly used for organoid culture.

MCE Serum Replacement is a fibrinogen-depleted and heparin-free Human Platelet Lysate. As a xeno-free and easy-to-use cell culture supplement, it can replace FBS (fetal bovine serum) to support the efficient and safe in vitro culture and expansion of various primary cells and cell lines.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell lines successfully cultured in DMEM include Hela, 293, Cos-7, and PC-12, as well as primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells.

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, etc.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell lines successfully cultured in DMEM include Hela, 293, Cos-7, and PC-12, as well as primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, etc.

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents.

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents.

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents.

MCE Cell-ATP Viability Detection Kit is used for detecting the number and viability of living cells in culture based on high-sensitivity bioluminescence detection technology of the ATP present.

MCE Protein A Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

RPMI 1640 contains glutathione and high concentration of vitamins, also contains biotin, vitamin B12, 4-Aminobenzoic acid (PABA) not found in MEM and DMEM, as well as high concentration of inositol and choline chloride.

MCE MCE Penicillin-Streptomycin (100×), Sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria.

MCE BM-Cyclin mainly contains Tiamulin fumarate and Minocycline hydrochloride, can effectively inhibit and remove mycoplasma contamination widespread in cell cultures. It is also effective against some common bacteria.

MCE Basement Membrane Matrix IPSC-qualified is primarily composed of natural basement membrane matrix extracted from mouse tumors. This product is mainly used for stem cells culture.

MCE Penicillin-Streptomycin-Glutamine (100×), sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria.

MCE Protein G Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

MCE Renilla-Firefly Luciferase Dual Assay Kit is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture.

MCE Penicillin-Streptomycin-Neomycin (PSN) (100×), Sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria.

MCE Basement Membrane Matrix (Phenol Red) is primarily composed of a natural basement membrane matrix derived from mouse tumors. This product is mainly for studies of tumor invasion, angiogenesis, and organoid culture.

MCE Gentamicin-Amphotericin B (500×), sterile is used to prevent contamination of cell culture due to their effective combined action against gram-positive bacteria, gram-negative bacteria, fungi and yeasts.

The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic.

MCE N-2 Serum-free Supplement (100×) can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12.

MCE Cytotoxicity LDH Assay Kit can detect LDH activity in the culture supernatant, which is indicative of cytotoxicity. Meanwhile viable cell number can be detected by CCK8 Kit (HY-K0301).

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cell culture media that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

MCE Basement Membrane Matrix is primarily composed of natural basement membrane matrix extracted from mouse tumors.This product is mainly used for studies of tumor invasion, angiogenesis and organoids culture while avoiding color interference in subsequent experiments.

N-2 Supplement promotes cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS).

MCE Basement Membrane Matrix GFR&HC is primarily composed of natural basement membrane matrix extracted from mouse tumors, is suitable for cell cultures that need to reduce growth factor induction and studies related to growth factors, signaling pathways, etc.

Bi-27 Additive (50×), Vitamin A-Free is a customized variant of the standard Bi-27 formulation with vitamin A removed as a supplement for neuronal cell culture to support the low- or high-density growth and short- or long-term viability of embryonic.

MCE Basement Membrane Matrix GFR (Phenol Red) is primarily composed of a natural basement membrane matrix derived from mouse tumors and contains lower levels of various cytokines than the standard type. This product is mainly used for organoid culture and for research applications with high requirements for basement membrane preparation.

MCE 0.25% Trypsin-EDTA (1x), phenol red consists of dried trypsin powder dissolved in EDTA. MCE Trypsin hydrolyzes intercellular proteins and disperses primary tissues or adherent cells into individual cells, and can be widely used for cell dissociation, cell culture passaging and primary tissue dissociation.

MCE Basement Membrane Matrix GFR is primarily composed of a natural basement membrane matrix derived from mouse tumors and contains less various cytokines than the standard type.This product is mainly used for organoid culture. It is also for research applications with high requirements for basement membrane preparation and has no effect on subsequent experiments requiring color detection.

L-glutamine is an important amino acid supplement commonly added to mammalian cell culture media. L-glutamine serves as an auxiliary energy source, especially when cells are rapidly dividing. L-glutamine is also important in the production of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, other amino acids, and plays a role in protein synthesis and glucose production.